Whole genome sequencing analysis of avian pathogenic Escherichia coli from China

Refining the definition of the avian pathogenic Escherichia coli (APEC) pathotype through inclusion of high-risk clonal groups

“…However, while we did not include broiler clinical isolates in this study, a wealth of literature exists for these E. coli populations. Multiple studies have reported the same dominant ST types in broiler clinical populations as those reported here for Turkey Clinical isolates, including ST23, ST117, ST131, ST155, ST355, and ST428 ( Danzeisen et al, 2013 ; Hussein et al, 2013 ; Pires-Dos-Santos et al, 2013 ; Maluta et al, 2014 ; Braga et al, 2016 ; Heidemann Olsen et al, 2016 ; Poulsen et al, 2018 ; Chen et al, 2021 ; Mageiros et al, 2021 ; Mehat et al, 2021 ). Furthermore, the broiler cecal and retail populations analyzed here support the presence of these same high-risk clones within broiler production, and ELA data indicates that they are similarly virulent in broilers compared to commercial turkeys.…”

Description and validation of a new set of PCR markers predictive of avian pathogenic Escherichia coli virulence

“…Little has been published about the importance of H4 in APEC. A recent study identified H4 in a high proportion (36%) of genomes of 125 strains isolated from chickens and ducks with obvious colibacillosis clinical symptoms in China (Chen et al, 2021). The emergence of two APEC strains belonging to clonal groups O111:H4-D-ST2085 and O111:H4-D-ST117 with high virulence-gene content and zoonotic potential has been reported in Spain (Mora et al, 2012).…”

Diagnosing Infectious Diseases in Poultry Requires a Holistic Approach: A Review

“…Sequence analysis of rRNA has been used for example for species identification of Campylobacter jejuni and Campylobacter coli [93], or Avibacterium paragallinarum [94]. NGS/WGS proved valuable in gaining more data regarding characterization of Morganella morganii [95], Ornithobacterium rhinotracheale [96], E. coli [97] and Salmonella Infantis [98]. These methods can also be applied for direct identification of bacterial communities as shown for samples from poultry carcasses in slaughterhouses [99].…”