Whole genome sequencing of Mycobacterium bovis to obtain molecular fingerprints in human and cattle isolates from Baja California, Mexico

Sandoval-Azuara, Sarai Estrella; Muñíz-Salazar, Raquel; Perea-Jacobo, Ricardo; Robbe‐Austerman, Suelee; Perera-Ortiz, Alejandro; López-Valencia, Gilberto; Bravo, Doris M; Sánchez-Flores, Alejandro; Miranda-Guzmán, Daniela; Flores-López, Carlos A.; Zenteno-Cuevas, Roberto; Laniado-Laborı́n, Rafael; Cruz, Fabiola Lafarga-De la; Stuber, Tod

doi:10.1016/j.ijid.2017.07.012

Cited by 30 publications

(36 citation statements)

References 46 publications

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“…This agrees with previous reports (Cobos-Marín et al, 2005;Santillán-flores et al, 2006;Reyes et al, 2008;Pérez-Guerrero et al, 2008;Bobadilla-del Valle et al, 2015;Sandoval-Azuara et al, 2017), where in spite of studying samples from different and specific regions of Mexico, the diversity of strains has been evident, suggesting an intense and continuous exchange of animals, and new genetic lines emerging as a consequence of the high prevalence of bTB in dairy cattle. The spoligoforest demonstrates all possible relations of spoligotypes under the assumption of spoligotype mutation, with genetic instability ranging from 10 to 20 years (Brosch et al, 2002;Gutiérrez et al, 2005;Smith et al, 2006).…”

Section: Discussionsupporting

confidence: 92%

Genetic diversity of Mycobacterium bovis in Jalisco, Mexico: Tracing back sources of infection

GonzÃ¡lez-Ruiz¹,

Sosa-Gallegos²,

RodrÃguez-HernÃ¡ndez³

et al. 2018

J. Vet. Med. Anim. Health

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Bovine tuberculosis (bTB) is a disease of cattle that presents risk to public health, causing severe economic losses to the livestock industry and difficulty in eradication because of its complex epidemiology. The aim of this study was to identify relationships between Mycobacterium bovis strains from cattle in the State of Jalisco, and those of other States of México. Molecular fingerprints of 337 M. bovis isolates from Jalisco, and 1152 from other States of México were included in the study. Isolates were obtained from tubercles between 1997 and 2015. Evolutionary relationship was determined throughout spoligoforest (www.emi.unsw.edu.au/spoltools/). From 337 isolates from Jalisco, 59 spoligotypes were obtained, ten of them included 48% of all isolates in the state. Five spoligotypes were common to beef and dairy cattle. The molecular analysis showed eight clusters in a philogenetic three: one with three subclusters of nine isolates each, all from dairy cattle; four with two isolates, including dairy and beef cattle. All spoligotypes from Jalisco have been reported in other states, four of the most frequent ones: SB0673, SB0971, SB0669 and SB0140, were the same as in other states. The most frequent spoligotypes of M. bovis found in Jalisco were also the most frequent ones in other parts of Mexico. However, there is no evidence to conclude that Jalisco is the source of infection to other states since no information on movement and destination of cattle could be documented.

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Section: Discussionsupporting

confidence: 92%

Genetic diversity of Mycobacterium bovis in Jalisco, Mexico: Tracing back sources of infection

GonzÃ¡lez-Ruiz¹,

Sosa-Gallegos²,

RodrÃguez-HernÃ¡ndez³

et al. 2018

J. Vet. Med. Anim. Health

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“…Since the whole-genome sequence of the first M. bovis strain became available in 2003 (Garnier et al, 2003), increasing efforts have been made to sequence additional strains and use whole-genome information to tackle bovine and/or wildlife TB transmission within specific outbreaks or countries (Bruning-Fann et al, 2017; Sandoval-Azuara et al, 2017; Ghebremariam et al, 2018; Kohl et al, 2018; Lasserre et al, 2018; Orloski et al, 2018a; Price-Carter et al, 2018; Razo et al, 2018). However, no studies to date have comprehensively analyzed M. bovis genomes at a global scale.…”

Section: Introductionmentioning

confidence: 99%

Global distribution and evolution ofMycobacterium bovislineages

Zimpel

Patané

Guedes

et al. 2019

Preprint

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AbstractMycobacterium bovis is the main causative agent of zoonotic tuberculosis in humans and frequently devastates livestock and wildlife worldwide. Previous studies suggested the existence of genetic groups of M. bovis strains based on limited DNA markers (a.k.a. clonal complexes), and the evolution and ecology of this pathogen has been only marginally explored at the global level. We have screened over 2,600 publicly available M. bovis genomes and newly sequenced two wildlife M. bovis strains, gathering 823 genomes from 21 countries and 21 host-species, including humans, to complete a phylogenomic analyses. We propose the existence of four distinct global lineages of M. bovis (Lb1, Lb2, Lb3 and Lb4) underlying the current disease distribution. These lineages are not fully represented by clonal complexes and are dispersed based on geographic location rather than host species. Our data divergence analysis agreed with previous studies reporting independent archeological data of ancient M. bovis [(South Siberian infected skeletons at ∼2,000 years BP (before present)] and indicates that extant M. bovis originated during the Roman period, subsequently dispersing across the world with the discovery and settlement of the New World and Oceania, directly influenced by trades among countries.

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“…Indications are that bTB causes a substantial global disease burden (WHO, ), but in the absence of reliable surveillance data it is impossible to assess the true incidence of M. bovis infection in humans, domestic cattle and camelids and various wildlife species. Although humans mostly acquire M. bovis infection from cattle (Amato et al., ; Sandoval‐Azuara et al., ), indications are that in some parts of the world cattle with presumed bTB may in fact be infected with Mycobacterium tuberculosis and not M. bovis (Hlokwe, Said, & Gcebe, ; Mittal et al., ; Sweetline, Ronald, Kumar, Kannan, & Thangavelu, ); reflecting likely human to cattle transmission of tuberculosis (TB). These examples of “reverse zoonosis” provide a powerful reminder that pathogen transmission between animals and humans is bidirectional.…”

Section: Introductionmentioning

confidence: 99%

BCG vaccination for bovine tuberculosis; conclusions from the Jerusalem One Health workshop

Marais

Buddle

Klerk‐Lorist

et al. 2018

Transbound Emerg Dis

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The global burden of bovine tuberculosis (bTB) remains poorly characterized, with spill‐over impacts on multiple species. The “One Health” concept is especially relevant given the bidirectional risk of cattle infecting humans with Mycobacterium bovis and humans infecting cattle with Mycobacterium tuberculosis. “Test and cull” is the traditional bTB control method, but the strategy may not be economically feasible or culturally acceptable where cattle are highly prized or their killing is a religious taboo; it is also less effective when there are wildlife reservoirs of infection. Vaccination with M. bovis bacille Calmette‐Guerin (BCG) provides protection against bTB, but its use in animals has been limited. The Jerusalem One Health workshop considered key bTB knowledge gaps and innovative solutions. Knowledge gaps identified included (a) the poorly quantified prevalence of M. bovis infection and disease in cattle, domestic camelids and human populations in developing countries, (b) the absence of alternatives to a “test and cull” strategy in settings where the killing of infected animals is culturally or economically unacceptable, or where affected species are protected and (c) an understanding of the induction of mucosal immunity against bTB. We summarize discussions on the use of BCG vaccination in domestic animals and wildlife and list potential projects to address the knowledge gaps identified.

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Whole genome sequencing of Mycobacterium bovis to obtain molecular fingerprints in human and cattle isolates from Baja California, Mexico

Cited by 30 publications

References 46 publications

Genetic diversity of Mycobacterium bovis in Jalisco, Mexico: Tracing back sources of infection

Genetic diversity of Mycobacterium bovis in Jalisco, Mexico: Tracing back sources of infection

Global distribution and evolution ofMycobacterium bovislineages

BCG vaccination for bovine tuberculosis; conclusions from the Jerusalem One Health workshop

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