Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, <i>Pinctada margaritifera</i>

Luyer, Jérémy Le; Auffret, Pauline; Quillien, Virgile; Leclerc, N.; Reisser, Céline; Vidal-Dupiol, Jérémie; Ky, Chin‐Long

doi:10.1101/421545

Cited by 3 publications

(2 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Raw reads were filtered with Trimmomatic V0.36.4 [ 29 ] to remove Illumina adapters (for Truseq3) and reads with an average Q-value below 26 for 95% of their length. To characterize and quantify the transcriptome of each sample, the filtered reads were paired-mapped against a P. margaritifera draft genome [ 30 ] with TopHat (V1.4.0). Cufflinks (V2.2.1.0) and Cuffmerge (V2.2.1.0) were used to assemble and merge the transcriptome produced for each library, respectively [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758)

Stenger

Reisser

et al. 2021

Genes

View full text Add to dashboard Cite

The shell color of the Mollusca has attracted naturalists and collectors for hundreds of years, while the molecular pathways regulating pigment production and the pigments themselves remain poorly described. In this study, our aim was to identify the main pigments and their molecular pathways in the pearl oyster Pinctada margaritifera—the species displaying the broadest range of colors. Three inner shell colors were investigated—red, yellow, and green. To maximize phenotypic homogeneity, a controlled population approach combined with common garden conditioning was used. Comparative analysis of transcriptomes (RNA-seq) of P. margaritifera with different shell colors revealed the central role of the heme pathway, which is involved in the production of red (uroporphyrin and derivates), yellow (bilirubin), and green (biliverdin and cobalamin forms) pigments. In addition, the Raper–Mason, and purine metabolism pathways were shown to produce yellow pigments (pheomelanin and xanthine) and the black pigment eumelanin. The presence of these pigments in pigmented shell was validated by Raman spectroscopy. This method also highlighted that all the identified pathways and pigments are expressed ubiquitously and that the dominant color of the shell is due to the preferential expression of one pathway compared with another. These pathways could likely be extrapolated to many other organisms presenting broad chromatic variation.

show abstract

Section: Methodsmentioning

confidence: 99%

Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758)

Stenger

Reisser

et al. 2021

Genes

View full text Add to dashboard Cite

show abstract

“…The value of a pearl, like any other valuable gem, is decided by its quality, which is decided by several qualities of the pearl such as shape, size, colour, lustre, and surface complexion [69,77]. Given that a pearl is the result of a complex biological phenomenon, diversity in pearls is unavoidable and is influenced by both the genetic makeup of the individual and the impact of many environmental factors.…”

Section: Pearl Quality Factors Affecting and Quality Enhancementmentioning

confidence: 99%

Recent Trends in Freshwater Pearl Farming in India

Saurabh¹,

Pradhan²,

Suman³

2022

Update on Malacology

View full text Add to dashboard Cite

Cultured pearls have an important place in international trade. The Vedas, the Bible, and the Koran all mentioned pearls, and they are regarded as one of the highest honours. Pearls are generated in nature when an irritant, such as a sand grain or a parasite, is swept into the pearl molluscs and lodged within it, where it is coated with micro-layers of nacre, a lustrous substance made up of 80–90 per cent aragonite crystals of CaCO3. The ICAR-Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, India, has created a base technology for cultivating pearls in freshwater habitats, recognising the scope and value of freshwater pearl production. Indian pond mussel, Lamellidens marginalis is the major species used in freshwater pearl aquaculture. In addition, ICAR-CIFA has pioneered a novel feature of freshwater pearl farming. The Institute has also taken the lead in disseminating freshwater pearl culture technology to the country’s fish farming communities, entrepreneurs, researchers, and students to build a sustainable model for the country’s socio-economic development. In this chapter, we will briefly cover pearls and their types, their historical significance, the spread of pearl mussels of freshwater origin in various countries, pearl biomineralisation, pearl farming techniques, and factors affecting pearl quality, among other things.

show abstract

Relationship of the orange tissue morphotype with shell and pearl colouration in the mollusc Pinctada margaritifera

Blay

Broustal

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Molluscs display a vast range of shell colours both between and within species. However, only a few species show colour variation in their soft tissues. In French Polynesia, the pearl oyster Pinctada margaritifera has three tissue morphotypes: the black wild-type and two rare mutations: white albino and orange mantle. Phenotypic transmission is known to occur from these phenotypes when they are used as graft donors for pearl production, leading to multicoloured and white pearls from black and albino mantle grafts, respectively. The present study furthers this knowledge by examining the phenotypic association between the orange mantle tissue morphotype and hard tissues: shells and cultured pearls. Based on a large experimental graft, shell colour quantification and pearl qualification showed that the orange morphotype is associated with light-coloured shells and pearls. Expression analysis of some candidate genes previously identified in the white mantle mutant, tested here on both graft and pearl sac tissues from orange mantle donors, confirmed the involvement of genes associated with shell matrix protein (shem4) and the melanin biosynthesis pathway (zinc). This study provides fundamental information on the mechanism behind mantle tissue colour in P. margaritifera and its association with biomineralisation and pigmentation processes that will be potentially valuable in future selection programs.

show abstract

Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera

Cited by 3 publications

References 91 publications

Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758)

Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758)

Recent Trends in Freshwater Pearl Farming in India

Relationship of the orange tissue morphotype with shell and pearl colouration in the mollusc Pinctada margaritifera

Contact Info

Product

Resources

About