Why do all lariat RNA introns have adenosine as the branch-point nucleotide? Conformational study of naturally-occuring branched trinucleotides and its eleven analogues by 1H-, 31P-NMR and CD spectroscopy

The formation of lariat RNAs in eukaryotes plays a central role in the transmission of genetic information into protein products through the synthesis of the processed RNA. The biomimetic synthetic models (branched and lariat RNAs) of the lariat RNA intron formed in the penultimate step of group 11 and nuclear pre-mRNA processing reaction (splicing) in eukaryotes have been used in structural studies (NMR) to understand its biological importance. Some of the synthetic lariat RNAs also function as biomimetic models for study of the self-cleavage reaction taking place in natural ribozyme-RNA substrate complexes. This review deals with our work on the design and synthesis of model systems mimicking the biological lariat RNA. All the published works on chemical synthesis of branched and lariat oligo-RNAs have been covered in this paper with emphasis on our own extensive work. Our synthetic schemes of acyclic branched RNA trimers (_1-7__), tetramers (8-15), pentamers (16, 17), heptamer t8, nonamer 1_2, 9 decamer 20 and lariat RNA tetramer pentamer 22, hexamer 23 and heptamer 24 show how delicately one had to employ different sets of complementary protecting groups and phosphorylation techniques for the synthesis of the target RNAs. Despite the display of apparent complexity of these strategies, they all can be grouped under two general reaction routes. In the final section we have given a brief summary of the results of our structural studies on our synthetic branched RNAs using 500 and 600 MHz NMR spectroscopy.

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Synthetic and conformational studies of branched and lariat RNAs. Modelling the lariat formed in the splicing reaction — A critical review

Sund

Glemarec

Chattopadhyaya

1994

Proc. Indian Acad. Sci. (Chem. Sci.)

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tRNA anticodons with the modified nucleoside 2-methylthio-N6-(4-hydroxyisopentenyl)adenosine distinguish between bases 3′ of the codon

Sollid

Björk

1991

Journal of Molecular Biology

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Regiodefined synthesis and conformational properties of adenyldiyl trimers with unsymmetrical 2′–5′ and 3′–5′ internucleotide linkages

1995

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Why do all lariat RNA introns have adenosine as the branch-point nucleotide? Conformational study of naturally-occuring branched trinucleotides and its eleven analogues by 1H-, 31P-NMR and CD spectroscopy

Cited by 14 publications

References 58 publications

Synthetic and conformational studies of branched and lariat RNAs. Modelling the lariat formed in the splicing reaction — A critical review

Synthetic and conformational studies of branched and lariat RNAs. Modelling the lariat formed in the splicing reaction — A critical review

tRNA anticodons with the modified nucleoside 2-methylthio-N6-(4-hydroxyisopentenyl)adenosine distinguish between bases 3′ of the codon

Regiodefined synthesis and conformational properties of adenyldiyl trimers with unsymmetrical 2′–5′ and 3′–5′ internucleotide linkages

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