Why do Tregs suddenly disappear in aplastic anemia?

Scheinberg, Phillip

doi:10.1182/blood.2020006446

Cited by 1 publication

(1 citation statement)

References 11 publications

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“…In recent years, numerous studies have demonstrated that cellular immunity plays a large role in the development of BM failure in AA [ 12 – 14 ], such as overactivation of CD8+ killer T-cells [ 15 ] and diminished immunosuppressive function of Tregs [ 16 , 17 ]. In our study, immune dysfunction in the model rats, including the downregulation of the CD4/CD8 ratio, decreased in CD4+CD25+FOXP3+ Tregs and the secretion of inflammatory factors TNF- α and IFN- γ increased, which reflected a negative regulation of haematopoiesis [ 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Sodium Chlorophyllin Copper on APO-1 Expression in Bone Marrow Mesenchymal Stem Cells of Rats with Aplastic Anaemia

Jin

Dai²,

Xuan

et al. 2022

Journal of Immunology Research

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Background. Aplastic anaemia (AA) is a highly prevalent blood disorder in the East and Southeast Asian countries, and a proportion of the patients is poorly treated with immunosuppressive agents. This study is aimed at exploring the effects of sodium copper chlorophyllin (SCC) on rats with AA and at providing the theoretical basis for the treatment of AA using traditional Chinese medicine. Methods. A rat model of AA was induced by combining 5-fluorouracil with busulfan, and different groups were treated with 25 mg/kg cyclosporin A (CsA) and low-, medium-, and high-dose SCC (25-, 50-, and 100-mg/kg; L-, M-, and H-SCC, respectively). A comparative analysis of peripheral blood counts, T-cell subsets, cytokine levels, bone marrow pathology, and APO-1 expression in mesenchymal stem cells in each group was conducted. Results. SCC can increase the platelet count and haemoglobin concentration in the peripheral blood of AA rats, whereas bone marrow biopsies revealed that the number of nucleated cells and megakaryocytes of SCC-treated rats increased compared with the model group. This was particularly evident in the H-SCC group. As regards the correction of immune function, unlike CsA, which reduced the absolute CD8+ T-cell count, SCC corrected the imbalanced CD4/CD8 ratio by increasing the absolute CD4+ T-cell count, whereas SCC increased the number of regulatory T-cells and reduced the level of interferon-γ in AA rats. When comparing the expression of APO-1 in the MSCs, results of the reverse-transcriptase polymerase chain reaction and Western blot analysis showed that SCC can increase the expression of APO-1 both at the mRNA and protein levels. Conclusion. We found that SCC can improve haematopoietic function and regress immune disorders in AA rats, which enhanced the expression of APO-1 in bone marrow MSCs. This may be one of the mechanisms of SCC in treating AA.

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