Why does the fallopian tube fail in ectopic pregnancy? The role of activins, inducible nitric oxide synthase, and MUC1 in ectopic implantation

Refaat, Bassem; Simpson, H. D.; Britton, Elizabeth G.; Biswas, Jhulan; Wells, Michael; Aplin, John D.; Ledger, William

doi:10.1016/j.fertnstert.2012.02.035

Cited by 29 publications

(22 citation statements)

References 46 publications

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“…Although we saw some disparity in our results, some recent studies have reported deregulation of MUC1 and altered glycosylation in the tubal epithelium in EPs compared to controls [27], [28]. Furthermore, another recent study reports a MUC-1 expression deficiency in five recurrent EP patients, highlighting the importance of proper regulation of this O-Glycan biosynthesis pathway [29].…”

Section: Discussioncontrasting

confidence: 79%

Embryonic miRNA Profiles of Normal and Ectopic Pregnancies

et al. 2014

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Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by real time PCR. Microarray studies showed that four miRNAs were differentially downregulated (hsa-mir-196b, hsa-mir-30a, hsa-mir-873, and hsa-mir-337-3p) and three upregulated (hsa-mir-1288, hsa-mir-451, and hsa-mir-223) in EP compared to control tissue samples. Hsa-miR-196, hsa-miR-223, and hsa-miR-451 were further validated by real time PCR in a wider population of EP and control samples. We also performed a computational analysis to identify the gene targets and pathways which might be modulated by these three differentially expressed miRNAs. The most significant pathways found were the mucin type O-glycan biosynthesis and the ECM-receptor-interaction pathways. We also checked that the dysregulation of these three miRNAs was able to alter the expression of the gene targets in the embryonic tissues included in these pathways such as GALNT13 and ITGA2 genes. In conclusion, analysis of miRNAs in ectopic and eutopic embryonic tissues shows different expression patterns that could modify pathways which are critical for correct implantation, providing new insights into the understanding of ectopic implantation in humans.

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Section: Discussioncontrasting

confidence: 79%

Embryonic miRNA Profiles of Normal and Ectopic Pregnancies

et al. 2014

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“…At the site of trophoblast invasion, MUC-1 expression was markedly reduced [77]. Lack or aberrant MUC-1 production was reported to be one of the reasons of ectopic pregnancy [78]. MUC-1 provides scaffold for L-selectin ligand, which binds L-selectin on the blastocyst surface, allowing loose physical contact between blastocyst and endometrium as well as facilitating blastocyst rolling over the endometrial surface [79].…”

Section: Role Of Lif In Embryo-endometrial Interactionmentioning

confidence: 99%

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

Salleh

Giribabu

2014

The Scientific World Journal

110

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Leukaemia inhibitory factor (LIF) plays an indispensible role in embryo implantation. Aberrant LIF production is linked to implantation failure. LIF regulates multiple processes prior to and during implantation such as uterine transformation into a receptive state, decidualization, blastocyst growth and development, embryo-endometrial interaction, trophoblast invasion, and immune modulation. Due to its critical role, LIF has been a target for a nonhormonal contraception. In this review, we summarize up-to-date information on the role of LIF in implantation and its role in contraception.

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“…Human Muc 1 expression is high during the peri-implantation period [40]. It is likely the first molecule that the embryo encounters before adhering to the endometrium [30, 41] (Figure 1(a)). In the uterus, Muc 1 suppresses the interaction between the implanting embryo and maternal endometrial adhesion molecules, thus creating a barrier to implantation in humans [30] as well as many other mammalian species [31].…”

Section: Regulation Of Mucin Expressionmentioning

confidence: 99%

Mucins Help to Avoid Alloreactivity at the Maternal Fetal Interface

Redžović

Laškarin

Dominović

et al. 2013

Clinical and Developmental Immunology

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During gestation, many different mechanisms act to render the maternal immune system tolerant to semi-allogeneic trophoblast cells of foetal origin, including those mediated via mucins that are expressed during the peri-implantation period in the uterus. Tumour- associated glycoprotein-72 (TAG-72) enhances the already established tolerogenic features of decidual dendritic cells with the inability to progress towards Th1 immune orientation due to lowered interferon (IFN)-γ and interleukin (IL)-15 expression. Mucine 1 (Muc 1) supports alternative activation of decidual macrophages, restricts the proliferation of decidual regulatory CD56+ bright natural killer (NK) cells, and downregulates their cytotoxic potential, including cytotoxic mediator protein expression. Removing TAG-72 and Muc 1 from the eutopic implantation site likely contributes to better control of trophoblast invasion by T cells and NK cells and appears to have important immunologic advantages for successful implantation, in addition to mechanical advantages. However, these processes may lead to uncontrolled trophoblast growth after implantation, inefficient defence against infection or tumours, and elimination of unwanted immunocompetent cells at the maternal-foetal interface. The use of mucins by tumour cells to affect the local microenvironment in order to avoid the host immune response and to promote local tumour growth, invasion, and metastasis confirms this postulation.

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Why does the fallopian tube fail in ectopic pregnancy? The role of activins, inducible nitric oxide synthase, and MUC1 in ectopic implantation

Cited by 29 publications

References 46 publications

Embryonic miRNA Profiles of Normal and Ectopic Pregnancies

Embryonic miRNA Profiles of Normal and Ectopic Pregnancies

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

Mucins Help to Avoid Alloreactivity at the Maternal Fetal Interface

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