Widespread increase in brain protein synthesis following acute immobilization stress in adult rat brain

Baubet, Valérie; Grange, Eric; Sermet, Eric; Giaume, Murielle; Bobillier, Pierre

doi:10.1016/s0304-3940(96)13209-2

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…The BLA was not monitored in this study. Baubet et al, (1996) refined this study to monitor translation 4 hr after a 2-hr AIS where they reported on higher S 35 methionine incorporation in BLA and CA1 and CA2 subfields of the hippocampus. Interestingly, even though these studies involved quantified autoradiographs that measured methionine incorporation levels over a large area, the levels of enhancement seen (2-3fold) are in the same range as we obtain with FUNCAT, which has a completely different detection procedure.…”

Section: Discussionmentioning

confidence: 99%

“…Surprisingly, analyses of translational profiles of the brain in stress‐mediated psychiatric diseases or models thereof remain scarce. Over two decades ago, two studies investigated the direct translational response to stress using S 35 incorporation kinetics of labelled methionine delivered intravenously (Baubet et al, ; Giaume et al, ). Of these Baubet et al, () looked at changes after acute stress using a venous delivery of labeled probe and measuring the partition kinetics between the brain and blood.…”

Section: Introductionmentioning

confidence: 99%

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Hippocampal and amygdalar cell‐specific translation is similar soon after stress but diverge over time

et al. 2018

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Stress is known to cause contrasting patterns of morphological and physiological plasticity in the hippocampus and amygdala. An obligatory cellular process underlying such neural changes is de novo translation and alterations in protein expression. Yet the nature of the translational response to stress in neurons remains largely unexplored. Even less is known about how glia are affected. Using a click-chemistry-based method to label the de novo proteome in live brain slices, we monitored translation in neurons and astrocytes of the basolateral amygdala (BLA) and dorsal hippocampal area CA3 (dCA3) in rats at different time-points after a single 2-hr exposure to immobilization stress. We observed enhancements in neuronal translation in both brain regions 1 hour after stress. This initial increase persisted in the BLA up to 10 days afterwards. In contrast, dCA3 neuronal translation gradually decreased to below control levels 10 days later. Translation profiles of dCA3 astrocytes followed timelines similar to neurons, but in BLA astrocytes translation peaked 1 day later and remained elevated 10 days later. Together our results demonstrate that stress causes an immediate upregulation of protein synthesis in both amygdalar and hippocampal neurons and astrocytes. However, these two areas eventually exhibit opposite temporal profiles of protein expression well after the end of stress. These findings identify new metrics of stress-induced plasticity at the level of cell-type specific proteomic landscape that may provide important insights into the molecular basis of the divergent temporal effects of stress across brain regions and biological scales.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hippocampal and amygdalar cell‐specific translation is similar soon after stress but diverge over time

et al. 2018

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“…Effects of null mutation for Fmr1 on rCPS (nmol ⅐ g ؊1 ⅐ min ؊1 ) in regions of white matter, cerebellum, pons, and medulla stress as a possible explanation for our results. Effects of stress on rCPS in rats have been reported (Giaume et al, 1995;Baubet et al, 1996). Children with FrX may be more sensitive to stress than their siblings (Wisbeck et al, 2000;Hessl et al, 2002), and Fmr1 null mice may be more sensitive to stress than WT (Beckel-Mitchener et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Postadolescent Changes in Regional Cerebral Protein Synthesis: AnIn VivoStudy in theFmr1Null Mouse

Qin

Kang²,

Burlin³

et al. 2005

J. Neurosci.

237

215

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“…The concentration of L-[35S]- methionine incorporation into proteins in these tissues was calculated based on tissue weight. To map the brain region-specific protein synthesis, serial coronal brain sections (20 µm) were made on the Cryostat and autoradiography of L-[35S]- methionine incorporation (exposed to Kodak Hyperfilm™ MP film, Amersham for 10 days) was conducted as described elsewhere [66]. The -[35S]- methionine incorporation signal was analyzed with an Olympus microscope (SZ-PT) and the Q-imaging system.…”

Section: Methodsmentioning

confidence: 99%

Post-Training Dephosphorylation of eEF-2 Promotes Protein Synthesis for Memory Consolidation

Nakajima

Gong

et al. 2009

PLoS ONE

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Memory consolidation, which converts acquired information into long-term storage, is new protein synthesis-dependent. As protein synthesis is a dynamic process that is under the control of multiple translational mechanisms, however, it is still elusive how these mechanisms are recruited in response to learning for memory consolidation. Here we found that eukaryotic elongation factor-2 (eEF-2) was dramatically dephosphorylated within 0.5–2 hr in the hippocampus and amygdala of mice following training in a fear-conditioning test, whereas genome-wide microarrays did not reveal any significant change in the expression level of the mRNAs for translational machineries or their related molecules. Moreover, blockade of NMDA receptors with MK-801 immediately following the training significantly impeded both the post-training eEF-2 dephosphorylation and memory retention. Notably, with an elegant sophisticated transgenic strategy, we demonstrated that hippocampus-specific overexpression of eEF-2 kinase, a kinase that specifically phosphorylates and hence inactivates eEF-2, significantly inhibited protein synthesis in the hippocampus, and this effects was more robust during an “ongoing” protein synthesis process. As a result, late phase long-term potentiation (L-LTP) in the hippocampus and long-term hippocampus-dependent memory in the mice were significantly impaired, whereas short-term memory and long-term hippocampus-independent memory remained intact. These results reveal a novel translational underpinning for protein synthesis pertinent to memory consolidation in the mammalian brain.

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Widespread increase in brain protein synthesis following acute immobilization stress in adult rat brain

Cited by 7 publications

References 16 publications

Hippocampal and amygdalar cell‐specific translation is similar soon after stress but diverge over time

Hippocampal and amygdalar cell‐specific translation is similar soon after stress but diverge over time

Postadolescent Changes in Regional Cerebral Protein Synthesis: AnIn VivoStudy in theFmr1Null Mouse

Post-Training Dephosphorylation of eEF-2 Promotes Protein Synthesis for Memory Consolidation

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