Ruminal protein degradability and intestinal rumen-undegraded protein digestibility (1DRUP) of whole-crop oat cut at: heading (H), milk (M) and milk-dough (M-D) stages of maturity were measu red. The experiment was conducted on three Holstein bulls fitted with ruminal and duodenal cannula using the in situ and mobile nylon bag methods.A significant effect of ensiling on rumen protein degradability parameters was shown. Ensiling increased both protein solubility (fraction A) and effective rumen degradability (ERD), decreased the content of potentially degradable fraction B (PO.001) and IDRUP (64.7 vs 55.3%; PO.001). The content of soluble fraction (A) and ERD increased, and the content of fraction (B) decreased from heading (H) to milk (M) stages. However, from M to M-D stages of growth, the content of fraction A (75.6 vs 70.5) and ERD (80.6 vs 78.2) decreased. Total tract protein digestibility decreased (PO.001) with increasing plant maturity. Moreover, IDRUP of M and M-D forages was lower than that of H forages (62.9, 56.3 and 56.6, respectively for H, M and M-D; PO.001).It can be concluded that in terms of protein availability from the gastrointestinal tract, the milk stage of growth seems to be the optimal harvest time.