Wireless Optogenetic Modulation of Cortical Neurons Enabled by Radioluminescent Nanoparticles

Chen, Zhaowei; Tsytsarev, Vassiliy; Finfrock, Y. Zou; Antipova, Olga; Cai, Zhonghou; Arakawa, Hiroyuki; Lischka, Fritz W.; Hooks, Bryan M.; Wilton, Rosemarie; Wang, Dongyi; Liu, Yi; Gaitan, Brandon; Yang, Tao; Chen, Yu; Erzurumlu, Reha S.; Yang, Huanghao; Rozhkova, Elena A.

doi:10.1021/acsnano.0c10436

Cited by 40 publications

(47 citation statements)

References 34 publications

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“…(C) Robotic arm guided implantation with magnetic resonance imaging, MRI ( Chen et al, 2019 ). (D) Upconversion nanoparticle, UPNC ( Wang et al, 2019 ), X-ray downconverting nanoparticle ( Chen et al, 2021 ), and photothermal-converting nanoparticle ( Chen X. et al, 2020 ; Qiao et al, 2021 ). (E) Optogenetic bioimplant ( Vasudevan et al, 2019 ).…”

Section: Methods Aiding Optogenetics In Neurosciencementioning

confidence: 99%

“…Therefore, downconverting nanoparticles could be the next solution for the penetration problem. One such system can convert X-rays into red light ( Figure 5D ; Chen et al, 2021 ). This system has been used to activate motor neurons in the primary motor cortex of mice with the red light-sensing opsin ReaChR ( Chen et al, 2021 ).…”

Section: Methods Aiding Optogenetics In Neurosciencementioning

confidence: 99%

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Red Light Optogenetics in Neuroscience

Lehtinen

Nokia

Takala

2022

Front. Cell. Neurosci.

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Optogenetics, a field concentrating on controlling cellular functions by means of light-activated proteins, has shown tremendous potential in neuroscience. It possesses superior spatiotemporal resolution compared to the surgical, electrical, and pharmacological methods traditionally used in studying brain function. A multitude of optogenetic tools for neuroscience have been created that, for example, enable the control of action potential generation via light-activated ion channels. Other optogenetic proteins have been used in the brain, for example, to control long-term potentiation or to ablate specific subtypes of neurons. In in vivo applications, however, the majority of optogenetic tools are operated with blue, green, or yellow light, which all have limited penetration in biological tissues compared to red light and especially infrared light. This difference is significant, especially considering the size of the rodent brain, a major research model in neuroscience. Our review will focus on the utilization of red light-operated optogenetic tools in neuroscience. We first outline the advantages of red light for in vivo studies. Then we provide a brief overview of the red light-activated optogenetic proteins and systems with a focus on new developments in the field. Finally, we will highlight different tools and applications, which further facilitate the use of red light optogenetics in neuroscience.

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Section: Methods Aiding Optogenetics In Neurosciencementioning

confidence: 99%

Section: Methods Aiding Optogenetics In Neurosciencementioning

confidence: 99%

Red Light Optogenetics in Neuroscience

Lehtinen

Nokia

Takala

2022

Front. Cell. Neurosci.

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“…Radioluminescent Gd 2 (WO 4 ) 3 :Eu nanoparticles can downconvert X-ray energy into photons with red-shifted light wavelength around 610 nm ( 408 ). Nanoparticles were well dispersed in an aqueous solution via surface modifications with ethylene glycol ligands to enhance the hydrophilicity.…”

Section: Physiological Modulation In Vivo By Wireless Optogeneticsmentioning

confidence: 99%

Optophysiology: Illuminating cell physiology with optogenetics

Tan

Huang

et al. 2022

Physiological Reviews

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Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions. Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution. Following the initial discovery of microbial opsins as light-actuated ion channels, a plethora of naturally occurring or engineered photoreceptors or photosensitive domains that respond to light at varying wavelengths has ushered in the next chapter of optogenetics. Through protein engineering and synthetic biology approaches, genetically-encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo. Here, we summarize these optogenetic tools on the basis of their fundamental photochemical properties to better inform the chemical basis and design principles. We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology"), and describe the current progress, as well as future trends, in wireless optogenetics, which enables remote interrogation of physiological processes with minimal invasiveness. This review is anticipated to spark novel thoughts on engineering next-generation optogenetic tools and devices that promise to accelerate both basic and translational studies.

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“…52 Recent advances have improved clinical translatability by incorporating biological formation processes and alternatives to device-based approaches, such as optogenetic approaches using radio-luminescent nanoparticles to stimulate genetically engineered light-gated neuron ion channels in vivo. 142 Genetic-based devices that interface with neural cells can be used to enable brain imaging, targeted drug delivery, and detection of specific biomolecules and ions. The ability to connect synthetic biology tools, specifically biomolecules and ion detection, shows possible directions of bioelectrical-based research of multifunctional devices.…”

Section: Outlook and Future Directionsmentioning

confidence: 99%