WISP-3 functions as a ligand and promotes superoxide dismutase activity

CCN6 regulates mitochondrial function

“…RNA and cDNA preparation, and PCR Total cellular RNA was isolated with the use of Trizol (Life Technologies) (Davis et al, 2006;Miller and Sen, 2007;Sen et al, 2004). The cDNA was prepared from RNA using cDNA synthesis kit (BioBharati Lifescience, India) as per the manufacturer's protocol.…”

“…CCN6, or Wnt-induced signaling protein 3 (WISP3), is a 354-aminoacid protein that can function both intracellularly and extracellularly (Brigstock et al, 2003;Davis et al, 2006;Miller and Sen, 2007;Pal et al, 2012;Perbal, 2013;Repudi et al, 2013). WISP3 gets its name from its sequence homology with other WISP (CCN)-family members that are induced by Wnt1 (Pennica et al, 1998;Sen et al, 2004).…”

CCN6 as a profibrotic mediator that stimulates the proliferation of lung fibroblasts via the integrin .BETA.1/focal adhesion kinase pathway

“…The C57BL/6 and MLg lung fibroblasts (8!10 3 cells per well) were cultured in 96-well flat-bottomed tissue plates in RPMI1640 supplemented with 10% FBS, and then serum-starved in medium containing 0.1% FBS overnight (19,20,22) [19,27,28]. Next, the cells were treated with various doses (1 to 200 ng/ml) of CCN6, and PDGF-BB (10 ng/ml) as a positive control for 72 hours, and labeled with 3 H -thymidine deoxyribose (TdR) (6.7 Ci/mmol ; Amersham Corp., Arlington Heights, IL) at 1 μCi/ well for the final 18 hours.…”

“…The ECM plays a vital early role in the repair process, orchestrating the modulation and transition of cell phenotype and function (26). It has been shown that CCN6 stimulates collagen type II, aggrecan, and superoxide dismutase expression and activity in chondrocytes (27,28). To investigate whether CCN6 affects on ECM production, we treated C57BL/6 cells with CCN6 and examined the mRNA expression of fibronectin and type I collagen by real-time RT-PCR analysis.…”

WISP3 - IGF1 interaction regulates chondrocyte hypertrophy

“…The disease manifestations linked to WISP3 mutations suggest that WISP3 is required for proper skeletal growth and maintenance of cartilage integrity (Hurvitz et al, 1999;Delague et al, 2005). Although the detailed molecular mechanism of the action of WISP3 is not resolved yet, existing functional studies justifying a role of WISP3 in skeletal development and cartilage maintenance are in accordance with the clinical and radiographic picture associated with WISP3 mutations (Hurvitz et al, 1999;Sen et al, 2004;Delague et al, 2005;Lamb et al, 2005;Davis et al, 2006;Miller and Sen, 2007;Nakamura et al, 2007;Baker et al, 2012;Garcia Segarra et al, 2012).…”