Wnt‐6 is expressed in the ureter bud and induces kidney tubule development in vitro

Itäranta, Petri; Lin, Yanfeng; Peräsaari, Juha; Roël, Giulietta; Destrée, Olivier; Vainio, Seppo

doi:10.1002/gene.10079

Cited by 102 publications

(79 citation statements)

References 36 publications

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“…Both Wnt6 and 9b are expressed throughout the Wolffian ducts and the ureteric bud/ proximal collecting duct system from E 10.5 through birth, although expression levels are lower in the ampullary tips (Fig. 3c,g,k,o and [42,43]). Wnt7b is weakly expressed in the Wolffian duct and the stalk of the UB but not in the vertical portion or tips of the bud at E 11.5 and E 12.5 ( Fig.…”

Section: Wnts In Renal Vesicle Formationmentioning

confidence: 99%

“…Analysis of mouse embryos by in situ hybridization has revealed the expression of six Wnts in the developing kidney: Wnt2b, Wnt4, Wnt6, Wnt7b, Wnt9b, and Wnt11 [9,[42][43][44][45]. Another eight Wnts have been shown to be present by sequencing of urogenital system cDNAs, with only Wnt5b identified as being specifically located in the kidney.…”

Section: The Role Of Wnts In Kidney Developmentmentioning

confidence: 99%

“…Wnt9b, on the other hand, cannot rescue Wnt4 mutant mesenchyme, suggesting that the two ligands signal through different receptors and/or branches of the pathway. However, Wnt6 can rescue the Wnt4 mutants, and Wnt6 expression levels are increased in Wnt4 mutants, presenting the possibility that it compensates for Wnt4 at later stages in Wnt4 null kidneys [43].…”

Section: Wnts In Renal Vesicle Formationmentioning

confidence: 99%

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Molecular regulation of kidney development: is the answer blowing in the Wnt?

2007

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Development of the metanephric kidney is a complicated process regulated by reciprocal signals from the ureteric bud and the metanephric mesenchyme that regulate tubule formation and epithelial branching morphogenesis. Over the past several years, several studies have suggested that Wnt signaling is involved in multiple aspects of normal kidney development as well as injury response and cancer progression. We will review these data here.

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Section: Wnts In Renal Vesicle Formationmentioning

confidence: 99%

Section: The Role Of Wnts In Kidney Developmentmentioning

confidence: 99%

Section: Wnts In Renal Vesicle Formationmentioning

confidence: 99%

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Molecular regulation of kidney development: is the answer blowing in the Wnt?

2007

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“…Within the kidney, Wnt6 plays a role in tubulogenesis (Itäranta et al, 2002), and can have both redundant and antagonistic functions with Wnt4 (Itä ranta et al, 2002). Within the developing ovary, the proper development of the Mü llerian ducts and the repression of testosterone production depend upon Wnt4 expression (Vainio et al, 1999;Jeays-Ward et al, 2003).…”

Section: Genital Ridge Expression Of Selectedmentioning

confidence: 99%

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Cory

Boyer

Pilon

et al. 2007

Molecular Reproduction Devel

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In mammals, the pre-Sertoli cell of the male genital ridge is the first cell type to display sex specific differentiation and differential gene expression. The genetic cascade driving the differentiationof pre-Sertoli cells and ultimately testis formationis beginning to be unravelled, but many questions remain. A better understanding of the transcriptome of pre-Sertoli cells immediately after sex determination is essential in order to further understand this differentiationprocess. A mouse model expressing Red Fluorescent Protein (RFP) under the control of a hybrid mouse/pig SRY promoter (HybSRYp-RFP) was used to purify cells from embryonic day 12.0 (e12.0) male genital ridges. To compare the transcriptomes of HybSRYp-RFP cell populations versus age matched whole female genital ridges, RNA was extracted and used to generate molecular probes that were hybridized onto Affymetrix Mouse Genome 430 2.0 microarrays. The expression of genes considered markers for pre-Sertoli cells, including Sox9, Mis, Dhh and Fgf9 were identified within the HybSRYp-RFP expressing cell population, while markers for germ cells (Oct4, SSEA-1) and endothelial cells (Ntrk3) were not identified. In contrast, markers for ovarian somatic cell expression, including Fst and Bmp2, were identified as overexpressed within the ovarian cell population. In a general fashion, genes identified as 2.5-fold over expressed in HybSRYp-RFP expressing cells coded notably for cell signalling and extra cellular proteins. The expression of Sox10, Stc2, Fgf18, Fgf13 and Wnt6 were further characterized via whole mount in situ hybridization (WISH) on male and female genital ridges between e11.5 and e14.5. Sox10, Fgf18, Fgf13 and Stc2 gene expression was detected within the male genital ridges while Wnt6 was found diffusely within both the male and female genital ridges. These data represent the earliest comprehensive microarray expression analysis of purified presumptive pre-Sertoli cells available to date. Mol. Reprod. Dev. 74: 1491-1504, 2007

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“…[1][2][3] The kidney provides an excellent developmental model organ because the early morphogenetic and cell differentiation steps noted in vivo are recapitulated ex vivo in organ culture conditions. 4 Moreover, the metanephric mesenchyme (MM) provides a way to target the mechanisms of nephrogenesis induced by Wnt signaling (for a review, see references [1][2][3][5][6][7][8][9][10][11].…”

mentioning

confidence: 99%

Functional Genetic Targeting of Embryonic Kidney Progenitor Cells Ex Vivo

Junttila

Saarela

Halt

et al. 2015

Journal of the American Society of Nephrology

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The embryonic mammalian metanephric mesenchyme (MM) is a unique tissue because it is competent to generate the nephrons in response to Wnt signaling. An ex vivo culture in which the MM is separated from the ureteric bud (UB), the natural inducer, can be used as a classic tubule induction model for studying nephrogenesis. However, technological restrictions currently prevent using this model to study the molecular genetic details before or during tubule induction. Using nephron segment-specific markers, we now show that tubule induction in the MM ex vivo also leads to the assembly of highly segmented nephrons. This induction capacity was reconstituted when MM tissue was dissociated into a cell suspension and then reaggregated (drMM) in the presence of human recombinant bone morphogenetic protein 7/human recombinant fibroblast growth factor 2 for 24 hours before induction. Growth factor-treated drMM also recovered the capacity for organogenesis when recombined with the UB. Cell tracking and time-lapse imaging of chimeric drMM cultures indicated that the nephron is not derived from a single progenitor cell. Furthermore, viral vector-mediated transduction of green fluorescent protein was much more efficient in dissociated MM cells than in intact mesenchyme, and the nephrogenic competence of transduced drMM progenitor cells was preserved. Moreover, drMM cells transduced with viral vectors mediating Lhx1 knockdown were excluded from the nephric tubules, whereas cells transduced with control vectors were incorporated. In summary, these techniques allow reproducible cellular and molecular examinations of the mechanisms behind nephrogenesis and kidney organogenesis in an ex vivo organ culture/organoid setting.

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Wnt‐6 is expressed in the ureter bud and induces kidney tubule development in vitro

Cited by 102 publications

References 36 publications

Molecular regulation of kidney development: is the answer blowing in the Wnt?

Molecular regulation of kidney development: is the answer blowing in the Wnt?

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Functional Genetic Targeting of Embryonic Kidney Progenitor Cells Ex Vivo

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