Wnt signaling in bone and muscle

Rudnicki, Michael A.; Williams, Bart O.

doi:10.1016/j.bone.2015.02.009

Cited by 119 publications

(96 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Wnt/β-catenin signaling is an essential pathway in the osteogenic differentiation of MSCs1415. Wnt signaling results in cellular accumulation of Wnt/β-catenin, followed by nuclear translocation of β-catenin and activation of target genes31. In this study, we detected higher expression of β-catenin following overexpression of HSPA1A during osteogenesis.…”

Section: Discussionsupporting

confidence: 52%

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Zhang

Xue

Yin

et al. 2016

Sci Rep

View full text Add to dashboard Cite

HSPA1A, which encodes cognate heat shock protein 70, plays important roles in various cellular metabolic pathways. To investigate its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), its expression level was compared between undifferentiated and differentiated BMSCs. Rat HSPA1A overexpression in BMSCs increased osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Moreover, it upregulated β-catenin and downregulated DKK1 and SOST. The enhanced osteogenesis due to HSPA1A overexpression was partly rescued by a Wnt/β-catenin inhibitor. Additionally, using a rat tibial fracture model, a sheet of HSPA1A-overexpressing BMSCs improved bone fracture healing, as determined by imaging and histological analysis. Taken together, these findings suggest that HSPA1A overexpression enhances osteogenic differentiation of BMSCs, partly through Wnt/β-catenin.

show abstract

Section: Discussionsupporting

confidence: 52%

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Zhang

Xue

Yin

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…supported that treatment with cordycepin in BMSCs activated the Wnt/β-catenin pathway, exerting an anti-oxidative property on bone metabolism [39, 42] and enhancing osteogenic differentiation. Recent studies support that the Wnt signaling pathway plays an important role in osteogenic differentiation and bone formation [44, 45]. Activation of canonical Wnt signaling results in higher bone density, whereas disruption of its activation impairs osteogenesis [46].…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

Chen¹,

Zhu²,

Xu³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Methods: Human bone mesenchymal stem cells (hBMSCs) were identified by multi-lineage differentiation. Alkaline phosphatase (ALP) activity, RT-PCR, western blots, immunofluorescent assay and Alizarin red staining of BMSCs were evaluated. A rat model of alcohol-induced ONFH was established to investigate the protective role of cordycepin against ethanol. Hematoxylin & eosin (H&E) staining and micro-computerized tomography (micro-CT) were performed to observe ONFH. Apoptosis was assessed by TdT-mediated dUTP nick end labeling (TUNEL). Immunohistochemical staining was carried out to detect OCN and COL1. Results: Ethanol significantly suppressed ALP activity, decreased gene expression of OCN and BMP2, lowered levels of RUNX2 protein, and reduced immunofluorescence staining of OCN and COL1 and calcium formation of hBMSCs. However, these inhibitory effects were attenuated by cordycepin co-treatment at concentrations of 1 and 10 µg/mL Moreover, it was revealed that the osteo-protective effect of cordycepin was associated with modulation of the Wnt/β-catenin pathway. In vivo, by micro-CT, TUNEL and immunohistochemical staining of OCN and COL1, we found that cordycepin administration prevented alcohol-induced ONFH. Conclusion: Cordycepin treatment to enhance osteogenesis may be considered a potential therapeutic approach to prevent the development of alcohol-induced ONFH.

show abstract

“…These molecules are capable to induce myogenic specification, which include Wnts, Sonic hedgehog (Shh), the Notch receptor and bone morphogenetic proteins (BMPs) (5,32). Wnt proteins constitute a large family of glycoproteins with several members shown to be required for early myogenesis in somites (33). The canonical Wnt signaling pathway requires Wnts binding to the Frizzled receptors to activate the β-catenin/TCF transcriptional complex (34).…”

Section: Regulation Of Mrfs By Signaling Moleculesmentioning

confidence: 99%

The myogenic regulatory factors, determinants of muscle development, cell identity and regeneration

Hernández-Hernández

García-González

Brun

et al. 2017

Seminars in Cell & Developmental Biology

Self Cite

481

316

View full text Add to dashboard Cite

The Myogenic Regulatory Factors (MRFs) Myf5, MyoD, myogenin and MRF4 are members of the basic helix-loop-helix family of transcription factors that control the determination and differentiation of skeletal muscle cells during embryogenesis and postnatal myogenesis. The dynamics of their temporal and spatial expression as well as their biochemical properties have allowed the identification of a precise and hierarchical relationship between the four MRFs. This relationship establishes the myogenic lineage as well as the maintenance of the terminal myogenic phenotype. The application of genome-wide technologies has provided important new information as to how the MRFs function to activate muscle gene expression. Application of combined functional genomics technologies along with single cell lineage tracing strategies will allow a deeper understanding of the mechanisms mediating myogenic determination, cell differentiation and muscle regeneration.

show abstract

Wnt signaling in bone and muscle

Cited by 119 publications

References 107 publications

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

The myogenic regulatory factors, determinants of muscle development, cell identity and regeneration

Contact Info

Product

Resources

About