Wnt6 expression in epidermis and epithelial tissues during <i>Xenopus</i> organogenesis

Lavery, Danielle L.; Davenport, Ian; Turnbull, Yvonne; Wheeler, Grant N.; Hoppler, Stefan

doi:10.1002/dvdy.21440

Cited by 23 publications

(28 citation statements)

References 35 publications

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“…As expected Wnts were detected in additional regions (Figures 1 and 2) with some of them reported earlier [18], [19], [20], [21], [22], [23], [24], [25], [26], [27], [28], [29], [30]: Wnt1 , Wnt2b , Wnt3a , Wnt8b , Wnt10a and Wnt10b are in the nervous system; Wnt5a , Wnt5b , Wnt7a , Wnt9a , Wnt11 , Wnt11b and Wnt16 are in the heart region; Wnt3a , Wnt7c , Wnt8a , Wnt8b , Wnt9a , Wnt9b and Wnt10b are in the epidermis; Wnt7a and Wnt7b are in the lateral plate mesoderm; Wnt11 and Wnt11b are in the somites; finally, Wnt3a and Wnt6 are in the ear.…”

Section: Resultssupporting

confidence: 85%

Expression of Wnt Signaling Components during Xenopus Pronephros Development

2011

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BackgroundThe formation of the vertebrate kidney is tightly regulated and relies on multiple evolutionarily conserved inductive events. These are present in the complex metanephric kidney of higher vertebrates, but also in the more primitive pronephric kidney functional in the larval stages of amphibians and fish. Wnts have long been viewed as central in this process. Canonical β-Catenin-dependent Wnt signaling establishes kidney progenitors and non-canonical β-Catenin-independent Wnt signaling participate in the morphogenetic processes that form the highly sophisticated nephron structure. While some individual Wnt signaling components have been studied extensively in the kidney, the overall pathway has not yet been analyzed in depth.Methodology/Principal FindingsHere we report a detailed expression analysis of all Wnt ligands, receptors and several downstream Wnt effectors during pronephros development in Xenopus laevis using in situ hybridization. Out of 19 Wnt ligands, only three, Wnt4, Wnt9a and Wnt11, are specifically expressed in the pronephros. Others such as Wnt8a are present, but in a broader domain comprising adjacent tissues in addition to the kidney. The same paradigm is observed for the Wnt receptors and its downstream signaling components. Fzd1, Fzd4, Fzd6, Fzd7, Fzd8 as well as Celsr1 and Prickle1 show distinct expression domains in the pronephric kidney, whereas the non-traditional Wnt receptors, Ror2 and Ryk, as well as the majority of the effector molecules are rather ubiquitous. In addition to this spatial regulation, the timing of expression is also tightly regulated. In particular, non-canonical Wnt signaling seems to be restricted to later stages of pronephros development.Conclusion/SignificanceTogether these data suggest a complex cross talk between canonical and non-canonical Wnt signaling is required to establish a functional pronephric kidney.

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Section: Resultssupporting

confidence: 85%

Expression of Wnt Signaling Components during Xenopus Pronephros Development

2011

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“…Instead, increased Wnt signaling in the pre-cardiac mesoderm prevents CM differentiation, which we hypothesize eventually leads to apoptotic death of the CPs via a p53/Caspase-3 independent mechanism. One possible explanation for this discrepancy is that in previous studies the CP and differentiation markers were assayed concurrently at later time points, not progressively over time points when the progenitor markers are first expressed (Afouda and Hoppler, 2009; Afouda et al, 2008; Foley et al, 2007; Foley and Mercola, 2005; Lavery et al, 2008a; Lavery et al, 2008b; Martin et al, 2010; Marvin et al, 2001; Naito et al, 2006; Tzahor and Lassar, 2001; Ueno et al, 2007). This leaves open the possibility that exploring additional time points could explain differences between this and previous studies.…”

Section: Discussionmentioning

confidence: 93%

Distinct phases of Wnt/β-catenin signaling direct cardiomyocyte formation in zebrafish

Dohn

Waxman

2012

Developmental Biology

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Normal heart formation requires reiterative phases of canonical Wnt/β-catenin (Wnt) signaling. Understanding the mechanisms by which Wnt signaling directs cardiomyocyte (CM) formation in vivo is critical to being able to precisely direct differentiated CMs from stem cells in vitro. Here, we investigate the roles of Wnt signaling in zebrafish CM formation using heat-shock inducible transgenes that increase and decrease Wnt signaling. We find that there are three phases during which CM formation is sensitive to modulation of Wnt signaling through the first 24 hours of development. In addition to the previously recognized roles for Wnt signaling during mesoderm specification and in the pre-cardiac mesoderm, we find a previously unrecognized role during CM differentiation where Wnt signaling is necessary and sufficient to promote the differentiation of additional atrial cells. We also extend the previous studies of the roles of Wnt signaling during mesoderm specification and in pre-cardiac mesoderm. Importantly, in pre-cardiac mesoderm we define a new mechanism where Wnt signaling is sufficient to prevent CM differentiation, in contrast to a proposed role in inhibiting cardiac progenitor (CP) specification. The inability of the CPs to differentiate appears to lead to cell death through a p53/Caspase-3 independent mechanism. Together with a report for an even later role for Wnt signaling in restricting proliferation of differentiated ventricular CMs, our results indicate that during the first 3 days of development in zebrafish there are four distinct phases during which CMs are sensitive to Wnt signaling.

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“…In most present studies, Wnt6, a canonical Wnt signaling molecule whose expression remained strong in the epithelium, regulated the development of many tissues like heart muscle (25) and somite formation (26). During the development of the anterior part of the chicken eye, Wnt6 was expressed in the entire ocular surface epithelium, but the authors (27) also found Wnt6 in the iridial mesenchyme at the final phase of development.…”

Section: Discussionmentioning

confidence: 95%