Wollaston prism-based structured illumination microscope with tunable frequency

Doblas, Ana; Bedoya, Sebastian; Preza, Chrysanthe

doi:10.1364/ao.58.0000b1

Cited by 5 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Integrated SIM offers simultaneous SR and OS functionalities by modifying the current SR-SIM reconstruction workflow. In addition to the laser beam interference-based SIM system, integrated SIM is also applicable to other SIM architectures, such as fringe projection-based SIM [30], grazing incidence SIM [11], prism SIM [26], etc. iSIM has its own limitations, however.…”

Section: Discussionmentioning

confidence: 99%

“…However, the inherent physical connection between the two modalities during the process of image reconstruction, particularly with regard to the suppression of the out-of-focus background, is still unclear. In addition, SIM with tunable frequencies has been reported to realize the combination of OS and SR [26], via the additional component of a Fresnel biprism.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Super-resolution and optical sectioning integrated structured illumination microscopy

Dan¹,

Gao²,

Zhao³

et al. 2020

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

Super-resolution structured illumination microscopy (SR-SIM) has attracted a great deal of attention in the past few decades. As a wide-field imaging technique, SR-SIM usually suffers from issues relating to out-of-focus background, particularly when imaging thick samples. In this study, we develop an integrated SIM with simultaneous SR and optical sectioning (OS) capabilities, facilitating SR imaging of stacked optical sections, with the out-of-focus background suppressed. The combination of the merits of SR and OS is realized by means of a new image reconstruction algorithm. We confirm the validity of the integrated SIM, both experimentally and in simulation. We anticipate that this integrated SIM will assist biologists in obtaining much clearer SR images in relation to thick specimens.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Super-resolution and optical sectioning integrated structured illumination microscopy

Dan¹,

Gao²,

Zhao³

et al. 2020

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

show abstract

“…Therefore, in our FB-based DHM system, the illuminating source should have a spectral bandwidth (

) no greater than 10 nm. 37 …”

Section: Polarization-sensitive Digital Holographic Microscopy Using mentioning

confidence: 99%

“…Therefore, in our FB-based DHM system, the illuminating source should have a spectral bandwidth (Δλ) no greater than 10 nm. 37 Since our DHM system operates in the telecentric regime, the FB is illuminated by a plane wave, thus the fringes' frequency in the FB-based DHM shown in Fig. 1(a) is independent of the biprism's position, p 0 ¼ 1∕2u 0 , where u 0 ¼ ðn − 1Þ tan δ∕λ ðn; δÞ are the biprism's refracting index and refringence angle, respectively.…”

Section: Introductionmentioning

confidence: 99%

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

et al. 2020

Self Cite

View full text Add to dashboard Cite

Significance: The hallmarks of digital holographic microscopy (DHM) compared with other quantitative phase imaging (QPI) methods are high speed, accuracy, spatial resolution, temporal stability, and polarization-sensitivity (PS) capability. The above features make DHM suitable for real-time quantitative PS phase imaging in a broad number of biological applications aimed at understanding cell growth and dynamic changes occurring during physiological processes and/or in response to pharmaceutical agents. Aim: The insertion of a Fresnel biprism (FB) in the image space of a light microscope potentially turns any commercial system into a DHM system enabling QPI with the five desired features in QPI simultaneously: high temporal sensitivity, high speed, high accuracy, high spatial resolution, and PS. To the best of our knowledge, this is the first FB-based DHM system providing these five features all together. Approach: The performance of the proposed system was calibrated with a benchmark phase object. The PS capability has been verified by imaging human U87 glioblastoma cells. Results: The proposed FB-based DHM system provides accurate phase images with high spatial resolution. The temporal stability of our system is in the order of a few nanometers, enabling live-cell studies. Finally, the distinctive behavior of the cells at different polarization angles (e.g., PS capability) can be observed with our system. Conclusions: We have presented a method to turn any commercial light microscope with monochromatic illumination into a PS QPI system. The proposed system provides accurate quantitative PS phase images in a new, simple, compact, and cost-effective format, thanks to the low cost (a few hundred dollars) involved in implementing this simple architecture, enabling the use of this QPI technique accessible to most laboratories with standard light microscopes.

show abstract

Development of a Wollaston phase grating polarized interferometer for simultaneous generation of interferograms

Reyes,

Rodríguez-Zurita,

Montes-Pérez

et al. 2025

Optics and Lasers in Engineering

View full text Add to dashboard Cite

Wollaston prism-based structured illumination microscope with tunable frequency

Cited by 5 publications

References 20 publications

Super-resolution and optical sectioning integrated structured illumination microscopy

Super-resolution and optical sectioning integrated structured illumination microscopy

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

Development of a Wollaston phase grating polarized interferometer for simultaneous generation of interferograms

Contact Info

Product

Resources

About