Working hypothesis to redefine endothelial progenitor cells

Prater, Daniel N.; Case, Jamie; Ingram, David A.; Yoder, Mervin C.

doi:10.1038/sj.leu.2404676

Cited by 274 publications

(233 citation statements)

References 114 publications

(181 reference statements)

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“…The results of the in vitro sprouting assay supported a synergistic effect of the two cell populations, likely because of the distinct participation of these two cell populations in the angiogenic process. EPCs appear to mainly contribute to cytokine production, whereas OECs also directly interact with native EC, supporting new blood vessel formation, as suggested by recent work in this field (34,46,47). OECs and EPCs both secrete a variety of angiogenic factors, and this likely causes the massive increase in EC migration when OECs and EPCs were cultured on top of gels containing ECs.…”

Section: Discussionmentioning

confidence: 85%

Material-based deployment enhances efficacy of endothelial progenitor cells

Silva

Kim

Kong

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

197

202

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Cell-based therapies are attractive for revascularizing and regenerating tissues and organs, but clinical trials of endothelial progenitor cell transplantation have not resulted in consistent benefit. We propose a different approach in which a material delivery system is used to create a depot of vascular progenitor cells in vivo that exit over time to repopulate the damaged tissue and participate in regeneration of a vascular network. Microenvironmental conditions sufficient to maintain the viability and outward migration of outgrowth endothelial cells (OECs) have been delineated, and a material incorporating these signals improved engraftment of transplanted cells in ischemic murine hindlimb musculature, and increased blood vessel densities from 260 to 670 vessels per mm 2 , compared with direct cell injection. Further, material deployment dramatically improved the efficacy of these cells in salvaging ischemic murine limbs, whereas bolus OEC delivery was ineffective in preventing toe necrosis and foot loss. Finally, material deployment of a combination of OECs with another cell population commonly isolated from peripheral or cord blood, endothelial progenitor cells (EPCs) returned perfusion to normal levels in 40 days, and prevented toe and foot necrosis. Direct injection of an EPC/OEC combination was minimally effective in improving limb perfusion, and untreated limbs underwent autoamputation in 3 days. These results demonstrate that vascular progenitor cell utility is highly dependent on the mode of delivery, and suggest that one can create new vascular beds for a variety of applications with this material-controlled deployment of cells.biomaterial ͉ cell therapy ͉ ischemic diseases ͉ neovascularization ͉ regenerative medicine

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Section: Discussionmentioning

confidence: 85%

Material-based deployment enhances efficacy of endothelial progenitor cells

Silva

Kim

Kong

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

197

202

View full text Add to dashboard Cite

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“…ECFCs may reside throughout the vascular endothelium contributing to generalized vascular integrity 15 . ECFCs are mobilized into a circulating pool of endothelial progenitors, potentially involved in neovasculogenesis via tissue recruitment and homing 16 , however the mechanism for this is not known at present. ECFCs may also contribute to new vessel formation during lung organogenesis as well as support lifelong lung repair and maintenance.…”

Section: Introductionmentioning

confidence: 99%

The isolation and culture of endothelial colony-forming cells from human and rat lungs

et al. 2015

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Blood vessels are crucial for the normal development, lifelong repair and homeostasis of tissues.Recently, vascular progenitor cell-driven "postnatal vasculogenesis" has been suggested as an important mechanism contributing to new blood vessel formation and organ repair. Among several described progenitor cell types contributing to blood vessel formation, endothelial colony forming cells (ECFCs) have received widespread attention as lineage-specific 'true' vascular progenitors. Herein, we describe a protocol for the isolation of pulmonary microvascular ECFCs from human and rat lung tissue. Our technique takes advantage of an earlier protocol for isolating circulating ECFCs from the mononuclear cellular fraction of peripheral blood. We

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“…In addition, endothelial cells can also arise from hemangioblasts, a common precursor for both hematopoietic and endothelial cells (His et al, 1900). In adults, endothelial precursor cells have been identified in bone marrow, peripheral blood and blood vessels (Prater et al, 2007). Two subsets of multipotential stem cells, HSCs and BMSCs/MSCs are resident in the postnatal bone marrow.…”

Section: Bmscs Based Postnatal De Novo Vasculogenesis and In Situ Vasmentioning

confidence: 99%