Workshop on Q fever

Skultéty, L

doi:10.4149/av_2017_314

Cited by 2 publications

(3 citation statements)

References 9 publications

(10 reference statements)

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“…In this study, we identified B-cell epitopes in antigen candidates for the development of serological tests for C. burnetii, and showed their immunogenicity in individuals naturally exposed to infection. In this way, we explored three C. burnetii outer membrane proteins (OMPs): OMP-H (CBU_0612), an outer membrane chaperon protein highlighted as a very strong immunodominant marker for both acute and persistent forms of Q fever [35,41]; Com-1 (CBU_1910), a strongly immunogenic protein [38,40] that is considered a reliable Q fever serodiagnosis marker [42,43]; and OMP-P1, a porin, highly expressed in large-cell variants (LCVs)-the replicative form of the bacterium in the cell hosts-and downregulated in small-cell variants (SCVs), the metabolically inactive and resistant form found in the environment [44][45][46]. However, the use of whole antigens in serodiagnosis may result in cross-reactivity with other related proteobacteria.…”

Section: Discussionmentioning

confidence: 99%

Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

et al. 2021

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Coxiella burnetii is a global, highly infectious intracellular bacterium, able to infect a wide range of hosts and to persist for months in the environment. It is the etiological agent of Q fever—a zoonosis of global priority. Currently, there are no national surveillance data on C. burnetii’s seroprevalence for any South American country, reinforcing the necessity of developing novel and inexpensive serological tools to monitor the prevalence of infections among humans and animals—especially cattle, goats, and sheep. In this study, we used immunoinformatics and computational biology tools to predict specific linear B-cell epitopes in three C. burnetii outer membrane proteins: OMP-H (CBU_0612), Com-1 (CBU_1910), and OMP-P1 (CBU_0311). Furthermore, predicted epitopes were tested by ELISA, as synthetic peptides, against samples of patients reactive to C. burnetii in indirect immunofluorescence assay, in order to evaluate their natural immunogenicity. In this way, two linear B-cell epitopes were identified in each studied protein (OMP-H(51–59), OMP-H(91–106), Com-1(57–76), Com-1(191–206), OMP-P1(197–209), and OMP-P1(215–227)); all of them were confirmed as naturally immunogenic by the presence of specific antibodies in 77% of studied patients against at least one of the identified epitopes. Remarkably, a higher frequency of endocarditis cases was observed among patients who presented an intense humoral response to OMP-H and Com-1 epitopes. These data confirm that immunoinformatics applied to the identification of specific B-cell epitopes can be an effective strategy to improve and accelerate the development of surveillance tools against neglected diseases.

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Section: Discussionmentioning

confidence: 99%

Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

et al. 2021

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“…Five proteins, four studied herein (CBU_1718, CBU_0092, CBU_0612, and CBU_0937) and one (CBU_1910) studied in an earlier study of ours were chosen as potential diagnostic markers. These proteins had been described in an earlier study by our team (Papadioti et al, 2011), and gained consideration according to further studies described in the literature (Sekeyova et al, 2010;Kowalczewska et al, 2012;Xiong et al, 2012;Wang et al, 2013;Skultety, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In adding to the mentioned above, Skultety ( 2017 ) described in their 2017 review that CBU_1718 (GroEL) appears to be a very reliable molecular marker for serodiagnosis of both acute and chronic Q fever and CBU_1910 (Com1) acts as an antigen which might induce protective immunity. Furthermore, it has been suggested that CBU_0092 (YbgF) is a phase II specific marker that can be employed for the early diagnosis of acute infection.…”

Section: Discussionmentioning

confidence: 99%

In the Search of Potential Serodiagnostic Proteins to Discriminate Between Acute and Chronic Q Fever in Humans. Some Promising Outcomes

Psaroulaki

Mathioudaki

Vranakis

et al. 2020

Front. Cell. Infect. Microbiol.

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Coxiella burnetii is the agent that causes acute and chronic Q fever infections in humans. Although the isolates studied so far have shown that the two forms of the disease differ in virulence potential thus, implying a variance in their proteomic profile, the methods used do not deliver enough discriminatory capability and often, human infections may be mis-diagnosed. The current study adds further knowledge to the results that we have already published on the Coxiella outer membrane protein 1 (Com1). Herein we identified the proteins GroEL, Ybgf, OmpH, and UPF0422 as candidates for serodiagnostics of Q fever; following cloning, expression and purification they were further used as antigens in ELISA for the screening of patients' sera associated with chronic Q fever endocarditis, sera negative for phase I IgG, sera with at least one sample positive for phase I IgG and sera from patients who suffered from various rheumatic diseases. Blood donors were used as the controls. Sensitivity, specificity, positive predictive value, negative predictive value, and Cohen's kappa coefficient (κ) were calculated and we also performed binary logistic regression analysis to identify combinations of proteins with increased diagnostic yield. We found that proteins GroEL and Ybgf, together with Com1, play the most significant role in the correct diagnosis of chronic Q fever. Of these three proteins, it was shown that Com1 and GroEL present the highest sensitivity and specificity altogether. The results add to the existing knowledge that an antigen-based serodiagnostic test that will be able to correctly diagnose chronic Q fever may not be far from reality.

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Workshop on Q fever

Abstract: no abstract Keywords: no keywords.

Cited by 2 publications

References 9 publications

Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

In the Search of Potential Serodiagnostic Proteins to Discriminate Between Acute and Chronic Q Fever in Humans. Some Promising Outcomes

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