Wound dressing components degrade proteins detrimental to wound healing

Baskovich, Brett; Sampson, Edith M.; Schultz, Gregory S.; Parnell, Laura K.S.

doi:10.1111/j.1742-481x.2007.00422.x

Cited by 26 publications

(21 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) showed similar degradation over time for both the control and test samples and were not significantly different (p = 0.5194), see Figure 4. The SearchLight technique results using pure TIMP-1 showed little degradation and are very similar to results previously reported using chronic wound fluid with an ELISA assay [27]. …”

Section: Resultssupporting

confidence: 86%

“…The blend of protease enzymes used in the dressings was tested for the ability to degrade, deactivate or activate known inflammatory or healing proteins. Some test results using a variety of methods and proteins were previously published [27]. Additional and repeat proteins using the same blend have been tested with a new technique to corroborate prior findings and are published herein.…”

Section: Introductionmentioning

confidence: 62%

“…Also, MMP9 showed a rapid degradation within 4 h, see Figure 2. Prior ELISA assay results of the protease blend incubated with chronic wound fluid with known MMP9 levels, showed complete MMP9 degradation within one hour [27]. Even though both assays showed rapid degradation, at this time, it is not known why there is a slight difference of time of degradation observed.…”

Section: Resultsmentioning

confidence: 97%

See 2 more Smart Citations

Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components

Parnell¹

2011

JFB

View full text Add to dashboard Cite

Chronic wounds typically have excessive levels of matrix metalloproteinases (MMPs) and proinflammatory cytokines that impair healing. Reducing these detrimental proteins may be key to healing chronic wounds. Proprietary protease blends were formulated specifically to degrade excessive amounts of proinflammatory factors that could prevent wound healing. Applications of protease-containing wound dressings to acute and chronic wounds have been observed clinically to resolve inflammation and appear to aid healing. The purpose of this study was to test in vitro a deliberate blend of proteases for the ability to deactivate or activate known proteins associated with inflammation or healing. Purified human target proteins were incubated with test and control solutions and samples removed at various time points. Blinded samples were tested using a novel infrared protein multiplex sandwich-ELISA-type array technique. Many proinflammatory proteins such as MMPs, cytokines and chemokines were susceptible to degradation. Many proteins such as growth factors, cytokines and TIMP1 were resistant to degradation. Not all proinflammatory proteins were deactivated. Family protein structure did not appear to affect susceptibility to degradation or deactivation. These results suggest that specific protease containing wound dressings appear to reduce multiple detrimental components which may disrupt their deleterious effects on the wound bed and microenvironment. By improving the wound microenvironment through the use of definitive proteases, these novel wound dressings may help transition wounds into the subsequent phase of healing.

show abstract

Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components

Parnell¹

2011

JFB

View full text Add to dashboard Cite

show abstract

“…To detach cells, proteases digest both membrane and ECM proteins [93], which results in delayed cell proliferation and differentiation. Moreover, proteases also degrade growth factors [94] and, if they remain in cell solution, they also lead to a delay in tissue regeneration.…”

Section: Temperature-responsive Dextran As a Cell-detaching Substratementioning

confidence: 99%

Engineering Dextran-Based Scaffolds for Drug Delivery and Tissue Repair

2012

View full text Add to dashboard Cite

Owing to its chemically reactive hydroxyl groups, dextran can be modified with different functional groups to form spherical, tubular and 3D network structures. The development of novel functional scaffolds for efficient controlled release and tissue regeneration has been a major research interest, and offers promising therapeutics for many diseases. Dextran-based scaffolds are naturally biodegradable and can serve as bioactive carriers for many protein biomolecules. The reconstruction of the in vitro microenvironment with proper signaling cues for large-scale tissue regenerative scaffolds has yet to be fully developed, and remains a significant challenge in regenerative medicine. This paper will describe recent advances in dextran-based polymers and scaffolds for controlled release and tissue engineering. Special attention is given to the development of dextran-based hydrogels that are precisely manipulated with desired structural properties and encapsulated with defined angiogenic growth factors for therapeutic neovascularization, as well as their potential for wound repair.

show abstract

“…Pathologies associated with MMP-9 include non-healing diabetic and nondiabetic wounds [5], atherosclerosis [16], aneurysm of Kawasaki disease, cerebrovascular accidents (CVAs), tumor growth, and metastasis [24].…”

Section: Discussionmentioning

confidence: 99%

Hyperbaric Oxygen Effect on MMP-9 After a Vascular Insult

Cummins

Gentene

2010

J. of Cardiovasc. Trans. Res.

View full text Add to dashboard Cite

Matrix metalloproteinease-9 (MMP-9) is involved in a host of processes. Many of its processes are physiologically beneficial as well as detrimental. The over-expression of this enzyme has been implicated as a contributory factor to some of the sequalae associated with cerebral ischemia, cell death, non-healing wounds, traumatic brain injury, aneurysms, and plaque instability in atherosclerosis. Several studies have examined the effect of hyperbaric oxygen (HBO) on MMP-9 expression. Because this proteinase is involved in both chronic and acute pathology, we wanted to investigate an acute expression model and see if, and how quickly, its expression would respond to HBO therapy. Our patient was scheduled to have elective surgery with an overnight stay followed by a series of HBO exposures. The patient served as her own control. An MMP-9 and urine pH was obtained prior to surgery to establish a baseline. On days 1, 3, and 4 post-op, samples were obtained before and after hyperbaric exposure. The patient was exposed to 100% O2 at 2.5 ATA for 60 min during each treatment for 5 days. The patient's MMP-9 values were dramatically elevated after surgery as compared to the baseline readings. The percentage increase from baseline was 400%. Our patient showed a significant reduction in MMP-9 expression after each hyperbaric exposure with the greatest decrease seen on post-op day 1 and subsequent exposures showing slightly less expression. Reduction in MMP-9 expression ranged from 46% on day 1 to 30% on post-op day 4. This case study suggests that if done relatively soon after a vascular or tissue insult, HBO can reduce MMP-9 expression. Chronic vascular pathologies, such as atherosclerotic plaque and aneurysms where over-expression of MMP-9 may result in acute coronary syndrome (ACS) or cerebral vascular accidents (CVAs), may be mitigated by a series of HBO treatments that reduce MMP-9 expression. Causality and/or contributory effects of MMP-9 expression in both pathologic and physiologic processes needs to be further elucidated. The understanding of how HBO therapy modulates these may provide an additional insight into mechanisms and future potential therapies for pathologic conditions such as those described above.

show abstract

Wound dressing components degrade proteins detrimental to wound healing

Cited by 26 publications

References 12 publications

Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components

Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components

Engineering Dextran-Based Scaffolds for Drug Delivery and Tissue Repair

Hyperbaric Oxygen Effect on MMP-9 After a Vascular Insult

Contact Info

Product

Resources

About