Wound healing can be improved by (—)‐epigallocatechin gallate through targeting Notch in streptozotocin‐induced diabetic mice

Huang, Yewei; Zhu, Quan; Yang, Xiaoying; Xu, Huanhuan; Sun, Bin; Wang, Xuanjun; Sheng, Jun

doi:10.1096/fj.201800337r

Cited by 82 publications

(61 citation statements)

References 34 publications

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“…One challenge in clinical medicine is the delayed healing of wounds; in particular, the delayed healing of acute wounds is always associated with a constant inflammatory state, resulting in slow recovery and economic and social burdens, especially for patients with diabetes mellitus (32). The incidence of chronic nonhealing wounds has been increasing in recent years (33). Under severe conditions, chronic diabetic ulcers of the feet and lower extremities will develop, and many patients with diabetes eventually require amputation.…”

Section: Discussionmentioning

confidence: 99%

Heparin‐poloxamer hydrogel‐encapsulated rhFGF21 enhances wound healing in diabetic mice

et al. 2019

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Wound healing, especially for diabetic wounds, is a lengthy and complicated process involving interactions and responses at the protein, cell, and tissue levels. Loading of growth factors into a hydrogel to construct a sustained‐release system is considered a promising approach to improve wound healing. The present study investigates the effect of thermosensitive heparin‐poloxamer (HP) hydrogel–encapsulated recombinant human fibroblast growth factor 21 (rhFGF21) on wound healing in mice with streptozotocin‐induced diabetes mellitus. First, we studied the in vitro release of rhFGF21 from the rhFGF21‐HP coacervate. The results showed that HP might control the release of rhFGF21. Next, we examined the effect of rhFGF21‐HP on skin wound healing in diabetic mice. Our data showed that rhFGF21‐HP significantly improved wound closure; promoted granulation, collagen deposition, and re‐epithelialization; and enhanced the expression of CD31. Moreover, rhFGF21‐HP had obvious advantages in diabetic wound healing. Therefore, the results suggest that the rhFGF21‐HP hydrogel polymer plays an important role in skin wound healing. This work provides a suitable sustained‐release delivery system that can continuously release rhFGF21 and presents a promising therapeutic strategy for wound healing in patients with diabetes.—Liu, H., Zhao, Y., Zou, Y., Huang, W., Zhu, L., Liu, F., Wang, D., Guo, K., Hu, J., Chen, J., Ye, L., Li, X., Lin, L. Heparin‐poloxamer hydrogel–encapsulated rhFGF21 enhances wound healing in diabetic mice. FASEB J. 33, 9858–9870 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

Heparin‐poloxamer hydrogel‐encapsulated rhFGF21 enhances wound healing in diabetic mice

et al. 2019

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“…The mice were housed in a controlled environment for two weeks before testing the BGLs. Mice with fasting BGLs > 17 mmol/L (about 306 mg/dL) were con rmed to be diabetic and kept for up to 16 weeks to develop diabetic nephropathy as previously described [17][18][19] .…”

Section: Diabetes Induction and Monitoringmentioning

confidence: 99%

“…The sections were randomly selected and stained with hematoxylin (Sigma, USA) and eosin (Solarbio, China) to evaluate re-epithelialization. The percentage of re-epithelialization [(distance traversed by epithelium over wound from wound edge/distance between wound edges) × 100] was calculated for two sections per wound and was averaged over sections to provide a representative value for each wound 22 . Mason's Trichrome (Solarbio, China) was used to stain sections for evaluation of Collagen deposition.…”

Section: Histochemical Stainingmentioning

confidence: 99%

“…Mason's Trichrome (Solarbio, China) was used to stain sections for evaluation of Collagen deposition. The positive area stained in each image was then quanti ed by image pro-plus 6.0 (MEDIA CYBERNETICS, Maryland, USA) counting the number of clearly stained pixels above the threshold intensity as previously described 18,23 . Images were captured using a Leica microscope and Leica CellSens Dimension software (Leica Microsystems, Wetzlar, Germany).…”

Section: Histochemical Stainingmentioning

confidence: 99%

“…The wound healing process was recorded as described in the methods section. The process of wound healing took 19 days, and the wound areas were then calculated 22 . The percentage of wound healing was measured, and we found that the wound healing was signi cantly delay in the DM group at days 4, 5, 6, 7, 8, and 9 ( Fig.…”

Section: Delay Wound Healing Appeared Following Administration Of Stzmentioning

confidence: 99%

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Dendrobium officinale Orchid Extract could improve wound healing in diabetic mice

Zhu¹,

Yu²,

Yang³

et al. 2020

Preprint

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Background In diabetes, delayed wound healing is one of the most prominent clinical manifestations and lacks satisfactory therapeutic schemes. Persistent inflammation impairs the healing process which occurs in the late phase of wound healing in diabetes mellitus (DM) mice. It has been report that dendrobium officinale (DE) could decrease inflammation and the NF-κB signaling pathway plays a critical role in regulated inflammation. The aim of the present study is to detect whether DE attenuated inflammation by NF-κB to accelerate wound healing in diabetes. Methods Mice were induced to diabetes by streptozocin (STZ) for 15 days. Two circular 8-mm full-thickness wounds were created on the back and then received DE treatment for 18 days. The percentage of wound healing, re-epithelialization and collagen deposition were calculation to evaluation the effect of DE on wound healing. The expression levels of interleukin (IL)- 1β and TNF-α in the wound site were determined by WB. Macrophage deposition and the type of macrophage were detected by WB and immunohistochemical staining. In addition, the expression of p65 was detected by WB. Results Herein, we observed that the late wound healing could be improved by dendrobium officinale orchid extract (DE) in streptozotocin (STZ)-induced DM mice. The accumulation of collagen was increased by DE treatment in the skin. We also found that the expression of pro-inflammation cytokines IL-1β, and TNF-α were inhibited by DE treatment in the skin wounds of DM mice. Furthermore, the macrophages accumulation which increased in diabetes wound site, were inhibited by DE treatment in the DM mice wound skin. And the CD163 which is a marker of type 2 macrophage was increased by DE treatment. Moreover, we confirmed that DE could decrease the expression of iNos which is a marker of type 1 macrophage by IHC in the skin wounds. And the levels of p65 and p65 phosphorylation were decreased by DE. Conclusions Wound healing can be improved by DE through NF-κB signaling pathway to attenuated inflammation in STZ-induced diabetic mice.

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Ultrahigh molecular weight polyethylene with improved crosslink density, oxidation stability, and microbial inhibition by chemical crosslinking and tea polyphenols for total joint replacements

Shah

Ren

Lan

et al. 2021

J of Applied Polymer Sci

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Highly crosslinked ultrahigh molecular weight polyethylene (UHMWPE) stabilized by vitamin E (VE) is widely applied in artificial joints as the bearings. Despite the approval, there is a discord that VE lowers the crosslinking efficiency, limiting its use at high concentration. In this work, we aim to obtain highly crosslinked and oxidation resistant UHMWPE through the conjunction of tea polyphenol and chemical crosslinking. We hypothesized that highly incorporated tea polyphenol with multiple reactive sites can ameliorate crosslinking efficiency of chemical crosslinked UHMWPE in comparison to VE. Epigallocatechin gallate (EGCG) as representative tea polyphenol was incorporated into UHMWPE at high concentration (2–8 wt%), followed by chemical crosslinking with 2 wt% organic peroxide. Unlike VE/UHMWPE blends as the control, chemical crosslinking achieved an increasing trend in crosslink density of EGCG/UHMWPE blends with increasing antioxidant concentration. High concentration of EGCG also enhanced the oxidation stability of UHMWPE. Intriguingly, EGCG endowed UHMWPE with an excellent antimicrobial property, which was inefficient in VE/UHMWPE. Cell viability was hardly affected by the high loaded antioxidant and peroxide. The chemically crosslinked UHMWPE blended with EGCG is proved to be a reasonable, cost effective and realistic alternative for use in artificial joints.

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Wound healing can be improved by (—)‐epigallocatechin gallate through targeting Notch in streptozotocin‐induced diabetic mice

Cited by 82 publications

References 34 publications

Heparin‐poloxamer hydrogel‐encapsulated rhFGF21 enhances wound healing in diabetic mice

Heparin‐poloxamer hydrogel‐encapsulated rhFGF21 enhances wound healing in diabetic mice

Dendrobium officinale Orchid Extract could improve wound healing in diabetic mice

Ultrahigh molecular weight polyethylene with improved crosslink density, oxidation stability, and microbial inhibition by chemical crosslinking and tea polyphenols for total joint replacements

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