WOX-ARF modules initiate different types of roots

Zhang, Teng; Ge, Yachao; Cai, Gui; Pan, Xuan; Xu, Lin

doi:10.1016/j.celrep.2023.112966

Cited by 22 publications

(6 citation statements)

References 51 publications

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“…In contrast, the auxin signals activating ARF7/ARF19 are thought to be developmentally regulated following endogenous rooting cues. Interestingly, both WOX11- and ARF7/ARF19-mediated root organogenesis pathways converge on LBD16 ( Okushima et al 2007 ; Sheng et al 2017 ; Zhang et al 2023 ). Our findings indeed show that cyst nematodes induce expression of LBD16 in a WOX11-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Transcription factor WOX11 modulates tolerance to cyst nematodes via adventitious lateral root formation

Willig,

Guarneri,

van Loon

et al. 2024

Plant Physiology

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The transcription factor WUSCHEL-RELATED HOMEOBOX 11 (WOX11) in Arabidopsis (Arabidopsis thaliana) initiates the formation of adventitious lateral roots upon mechanical injury in primary roots. Root-invading nematodes also induce de novo root organogenesis leading to excessive root branching, but it is not known if this symptom of disease involves mediation by WOX11 and if it benefits the plant. Here, we show with targeted transcriptional repression and reporter gene analyses in Arabidopsis that the beet cyst nematode Heterodera schachtii activates WOX11-mediated adventitious lateral rooting from primary roots close to infection sites. The activation of WOX11 in nematode-infected roots occurs downstream of jasmonic acid-dependent damage signaling via ETHYLENE RESPONSE FACTOR109, linking adventitious lateral root formation to nematode damage to host tissues. By measuring different root system components, we found that WOX11-mediated formation of adventitious lateral roots compensates for nematode-induced inhibition of primary root growth. Our observations further demonstrate that WOX11-mediated rooting reduces the impact of nematode infections on aboveground plant development and growth. Altogether, we conclude that the transcriptional regulation by WOX11 modulates root system plasticity under biotic stress, which is one of the key mechanisms underlying tolerance of Arabidopsis to cyst nematode infections.

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Section: Discussionmentioning

confidence: 99%

Transcription factor WOX11 modulates tolerance to cyst nematodes via adventitious lateral root formation

Willig,

Guarneri,

van Loon

et al. 2024

Plant Physiology

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“…Both Wuschel-related Homeobox 9 ( WOX9 ) and ARF5 /MP are required for hypophysis specification and primary root formation, with mutations in either WOX9 or ARF5 /MP resulting in defective stem cell niche establishment of the primary root ( Breuninger et al., 2008 ). The WOX9-ARF5/MP complex initiates primary root formation by activating RGF1 INSENSITIVEs (RGIs) in the primary root founder cell ( Zhang et al., 2023 ). Root cap formation in Arabidopsis is regulated by miRNA160, which targets ARF10 and ARF16.…”

Section: Arf-mediated Auxin Pathway In Growth and Developmentmentioning

confidence: 99%

Enigmatic role of auxin response factors in plant growth and stress tolerance

Liu,

Yahaya,

et al. 2024

Front. Plant Sci.

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Abiotic and biotic stresses globally constrain plant growth and impede the optimization of crop productivity. The phytohormone auxin is involved in nearly every aspect of plant development. Auxin acts as a chemical messenger that influences gene expression through a short nuclear pathway, mediated by a family of specific DNA-binding transcription factors known as Auxin Response Factors (ARFs). ARFs thus act as effectors of auxin response and translate chemical signals into the regulation of auxin responsive genes. Since the initial discovery of the first ARF in Arabidopsis, advancements in genetics, biochemistry, genomics, and structural biology have facilitated the development of models elucidating ARF action and their contributions to generating specific auxin responses. Yet, significant gaps persist in our understanding of ARF transcription factors despite these endeavors. Unraveling the functional roles of ARFs in regulating stress response, alongside elucidating their genetic and molecular mechanisms, is still in its nascent phase. Here, we review recent research outcomes on ARFs, detailing their involvement in regulating leaf, flower, and root organogenesis and development, as well as stress responses and their corresponding regulatory mechanisms: including gene expression patterns, functional characterization, transcriptional, post-transcriptional and post- translational regulation across diverse stress conditions. Furthermore, we delineate unresolved questions and forthcoming challenges in ARF research.

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“…In A. thaliana , after plant organ wounding, the auxin signaling pathway activates the expression of WOX11 in the future root founder cell [ 3 ]. In its turn, WOX11 triggers the expression of other genes ( LBD16 , WOX5 , and WOX7 ), which initiate the generation of adventitious roots and/or calli [ 4 , 5 ]. Inhibition of the WOX11 pathway results in decreased rooting ability, whereas overexpression of WOX11 increases the ability of detached leaves to form roots [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, WOX11 ortholog in rice was also recently shown to directly activate expression of CKX4 , thereby stimulating crown root development [ 9 ]. In A. thaliana , WOX9 interacting with ARF5 (also known as MONOPTEROS, MP) in hypophysis takes part in primary root initiation, while the WOX11-ARF6/8 complex regulates the fate of an adventitious root founder cell [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

The WOX Genes from the Intermediate Clade: Influence on the Somatic Embryogenesis in Medicago truncatula

Yakovleva,

Efremova,

Smirnov

et al. 2024

Plants

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Transcription factors from the WOX family are well-known regulators of cell proliferation and differentiation in plants. Herein, we focused on several WOX genes from the intermediate clade and checked their impact on somatic embryogenesis using the model legume object Medicago truncatula. As a result, we show that MtWOX9-1 overexpression not only stimulates somatic embryogenesis in the embryogenic M. truncatula line, as it was shown previously, but can also induce somatic embryogenesis in the non-embryogenic line. Other intermediate clade WOX, including the close paralog of MtWOX9-1, as well as WOX11 homologs, did not have any significant impact on somatic embryogenesis in our in vitro cultivation system. Together, our results give new information about the diversity of the WOX family proteins and their specific functions. These data can be used for the search of new regeneration stimulators.

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WOX-ARF modules initiate different types of roots

Cited by 22 publications

References 51 publications

Transcription factor WOX11 modulates tolerance to cyst nematodes via adventitious lateral root formation

Transcription factor WOX11 modulates tolerance to cyst nematodes via adventitious lateral root formation

Enigmatic role of auxin response factors in plant growth and stress tolerance

The WOX Genes from the Intermediate Clade: Influence on the Somatic Embryogenesis in Medicago truncatula

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