Wrapping collagen-based nanoparticle with macrophage membrane for treating multidrug-resistant bacterial infection

Abstract

Nowadays, multidrug-resistant (MDR) bacterial infectious diseases has become a thorny issue in the healthcare field. Owning to its intrinsic merits, photodynamic therapy (PDT) shows tremendous strengths in fighting against MDR bacterial infections. However, most photodynamic nanoplatforms exhibit unsatisfactory targeting efficiency towards bacteria and infection site, which may compromise the bactericidal effect of PDT. Herein, we firstly reported a bacteria-targeted collagen-based nanoparticle, named Ce6/Col/MM, for treating methicillin-resistant Staphylococcus aureus (MRSA)-infected wound. Ce6/Col/MM was fabricated by wrapping chlorin e6 (Ce6)-loaded collagen-based nanoparticles with macrophage membrane (MM), showing excellent photodynamic activity and good biocompatibility. In vitro studies demonstrated that Ce6/Col/MM could target to bacteria and then exhibit prominent antibacterial capacity against planktonic MRSA under light irradiation. Furthermore, the treatment of MRSA-infected wound in mice with Ce6/Col/MM plus light illumination resulted in potent bacterial inactivation and accelerated wound healing, accompanied by favorable histological compatibility. Collectively, Ce6/Col/MM with superior targeting ability towards bacteria, effective photodynamic antibacterial potency and minimal safety concerns, might be a powerful bactericidal nanoagent for treating infections caused by MDR bacteria. Graphical Abstract

Highly Efficient Photodynamic Hydrogel with AIE-Active Photosensitizers toward Methicillin-Resistant Staphylococcus aureus Ultrafast Imaging and Killing

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsbiomaterials.4c00056. Detailed experimental section; synthesis process, 1 H NMR spectrum, 13…”

“…aureus. , Due to the overuse and misuse of antibiotics and lack of new drug development, MRSA has increased dramatically throughout the world in recent decades and caused outbreaks and epidemics, leading to increased mortality. , According to statistics from the World Health Organization, 10 million people are expected to die from drug-resistant bacterial infections by 2050 . Therefore, in order to cope with the impending disaster caused by drug-resistant bacteria, it is urgent to seek and develop new therapeutic solutions …”

“…12 Therefore, in order to cope with the impending disaster caused by drugresistant bacteria, it is urgent to seek and develop new therapeutic solutions. 13 In recent years, photodynamic treatment (PDT), a no-drug resistance therapy, showed satisfactory results to combat drugresistant bacteria. 14−19 Antibacterial PDT can produce large amounts of reactive oxygen species (ROS) under specific laser irradiation.…”

Macrophage membrane-coated nanoparticles for the treatment of infectious diseases

“…Some studies have combined macrophage membrane coating technology with photodynamic therapy (PDT) or photothermal therapy (PTT) to achieve targeted killing of pathogens [86,97,98]. For instance, Li and co-workers prepared a macrophage membrane coated with the photosensitizer chlorin e6 (Ce6/Col/MM) (figure 5(A)) [99]. They confirmed that CD14 on the Ce6/Col/MM surface enabled specific conjugation with MRSA (figures 5(B) and (C)).…”

Amphiphilic di-cationic methylene blue for improving antibacterial photodynamic efficiency through high accumulation and low aggregation on bacterial cell surfaces

“…Therefore, in the delivery system of methylene blue, the content of monomer state is improved by various methods, such as reducing the concentration, using surfactants, using liposomes and so on. [43][44][45] However, little attention is paid to the fact that whether methylene blue can maintain the monomer state with high photodynamic efficiency aer adsorption on the cell surface. In order to compare the possible mechanism of APDT of amphiphilic photosensitizer C 12 -MB, we studied the photosensitizer aggregation on the surface of bacterial cells by spectroscopy, and the results are shown in Fig.…”