Wrinkling Non-Spherical Particles and Its Application in Cell Attachment Promotion

Li, Minggan; Joung, Dehi; Hughes, Bethany R.; Waldman, Stephen D.; Koziński, Janusz A.; Hwang, Dae Kun

doi:10.1038/srep30463

Cited by 50 publications

(80 citation statements)

References 41 publications

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“…The introduction of specific subcellular scaled surface features often promotes cell adhesion. The enhanced cell attachment of 3T3 cells on wrinkled surfaces having similar size and morphology was reported [14,24]. Thus, promotion of cell attachment by wrinkles is considered one reason for the higher cell number.…”

Section: T3 Cell Culture On the Wrinkle-cs Filmsupporting

confidence: 61%

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

Izawa

Okuda

Yonemura

et al. 2018

Colloids and Interfaces

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Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 • C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress.

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Section: T3 Cell Culture On the Wrinkle-cs Filmsupporting

confidence: 61%

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

Izawa

Okuda

Yonemura

et al. 2018

Colloids and Interfaces

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“…Wrinkled surfaces of cell cultured scaffolds with suitable topographies can improve cell proliferation, 51 cellular alignment, and differentiation control. 52,53 It seems that wrinkles are under the impression of mechanico-chemical properties of the construct such as hydrophilicity, porosity, tensile strength and in vivo/in vitro conditions. Protein adsorption is another important property since the number of serum proteins absorbed by the scaffold that can affects cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Golchin

Hosseinzadeh

Staji

et al. 2019

J of Cellular Biochemistry

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Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio. In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers. Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests. Furthermore, viability of the buccal fat pad‐derived mesenchymal stem cells (BFP‐MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds. Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate. Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation. Cur release profile showed a decreasing effect on BFP‐MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long‐term manner. In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP‐MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.

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“…Bottom-up methods 12 , including blockcopolymer self-assembly 13 or a range of surface instabilities 14 provide thus attractive alternatives. Mechanically-induced wrinkling of bilayers [15][16][17] has been extensively used for a plethora of applications in surface science, optics and photonics, biology and microfabrication [18][19][20] . These range from the fabrication of super-hydrophobic or directional wetting surfaces 21 , to tuneable lasers 22 and cell sorting 23 and proliferation 24 .…”

Section: Introductionmentioning

confidence: 99%

Sub-100 nm wrinkling of polydimethylsiloxane by double frontal oxidation

et al. 2017

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We demonstrate nanoscale wrinkling on polydimethylsiloxane (PDMS) at sub-100 nm length scales via a (double) frontal surface oxidation coupled with a mechanical compression. The kinetics of the glassy skin propagation is resolved by neutron and X-ray reflectivity, and atomic force microscopy, combined with mechanical wrinkling experiments to evaluate the resulting pattern formation. In conventional PDMS surface oxidation, the smallest wrinkling patterns attainable have an intrinsic lower wavelength limit due to the coupling of skin formation and front propagation at fixed strain ε prestrain , whose maximum is, in turn, set by material failure. However, combining two different oxidative processes, an ultra-violet ozonolysis followed by air plasma exposure, we break this limit by fabricating trilayer laminates with excellent interfacial properties and a sequence of moduli and layer thicknesses able to trivially reduce the surface topography to sub-100 nm dimensions. This method provides a powerful, yet simple, non-lithographic approach to extend surface patterning from visible to the deep UV range.

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Wrinkling Non-Spherical Particles and Its Application in Cell Attachment Promotion

Cited by 50 publications

References 41 publications

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Sub-100 nm wrinkling of polydimethylsiloxane by double frontal oxidation

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