Writing and Functionalisation of Suspended DNA Nanowires on Superhydrophobic Pillar Arrays

Miele, Ermanno; Accardo, Angelo; Falqui, Andrea; Marini, Monica; Giugni, Andrea; Leoncini, Mauro; Angelis, Francesco De; Krahne, Roman; Fabrizio, E. Di

doi:10.1002/smll.201401649

Cited by 31 publications

(29 citation statements)

References 49 publications

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“…However, these approaches require careful handling only to generate nanowires that are randomly positioned and oriented. Templates whose surfaces are decorated with patterns, such as microwells (10), nanogratings (11), and micropillars (12,13), have been shown to guide the location of nanowires. However, the experimental approaches for creating such patterns largely rely on lithography-based methods, which involve complex processes that are not readily accessible.…”

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confidence: 99%

Spontaneous formation of aligned DNA nanowires by capillarity-induced skin folding

Nagashima

Kim

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Although DNA nanowires have proven useful as a template for fabricating functional nanomaterials and a platform for genetic analysis, their widespread use is still hindered because of limited control over the size, geometry, and alignment of the nanowires. Here, we document the capillarity-induced folding of an initially wrinkled surface and present an approach to the spontaneous formation of aligned DNA nanowires using a template whose surface morphology dynamically changes in response to liquid. In particular, we exploit the familiar wrinkling phenomenon that results from compression of a thin skin on a soft substrate. Once a droplet of liquid solution containing DNA molecules is placed on the wrinkled surface, the liquid from the droplet enters certain wrinkled channels. The capillary forces deform wrinkles containing liquid into sharp folds, whereas the neighboring empty wrinkles are stretched out. In this way, we obtain a periodic array of folded channels that contain liquid solution with DNA molecules. Such an approach serves as a template for the fabrication of arrays of straight or wrinkled DNA nanowires, where their characteristic scales are robustly tunable with the physical properties of liquid and the mechanical and geometrical properties of the elastic system. DNA nanowires | capillary forces | wrinkling | folding | instability A ssembling biomolecules and microorganisms (e.g., virus and DNA) into a desired architecture has offered new routes to the fabrication of nanomaterials (1, 2). In particular, DNA nanowires have proven useful as a template to fabricate functional nanomaterials and as a platform for genetic analysis (3-5). Molecular combing and its derivatives have been used widely to obtain such nanowires using an aqueous solution of DNA molecules, where capillary forces of the solution at a receding meniscus act to stretch and immobilize the molecules on a solid surface (6). To manipulate the size, geometry, and alignment of nanowires, much effort has been devoted to controlling the evaporation of the solutions by adjusting experimental parameters, such as concentration and temperature, or applying external forces that move the droplets in a desired direction (7-9). However, these approaches require careful handling only to generate nanowires that are randomly positioned and oriented. Templates whose surfaces are decorated with patterns, such as microwells (10), nanogratings (11), and micropillars (12, 13), have been shown to guide the location of nanowires. However, the experimental approaches for creating such patterns largely rely on lithography-based methods, which involve complex processes that are not readily accessible.Mechanical instabilities that occur in response to external stimuli (e.g., loading and heat) and geometric constraints cause surface wrinkling and folding of skin-substrate systems, which are ubiquitous in nature and have been harnessed to create wrinkled and folded materials for versatile applications (14). The transition from wrinkling to folding occurs when the misma...

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confidence: 99%

Spontaneous formation of aligned DNA nanowires by capillarity-induced skin folding

Nagashima

Kim

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Using molecular combing, DNA bundles can be stretched and assembled between micromachined structures353637. Electrophoretic stretching is another technique to stretch DNA molecules between two micromachined structures3839.…”

Section: Methodsmentioning

confidence: 99%

A rapid and practical technique for real-time monitoring of biomolecular interactions using mechanical responses of macromolecules

Tarhan

Lafitte

Tauran

et al. 2016

Sci Rep

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Monitoring biological reactions using the mechanical response of macromolecules is an alternative approach to immunoassays for providing real-time information about the underlying molecular mechanisms. Although force spectroscopy techniques, e.g. AFM and optical tweezers, perform precise molecular measurements at the single molecule level, sophisticated operation prevent their intensive use for systematic biosensing. Exploiting the biomechanical assay concept, we used micro-electro mechanical systems (MEMS) to develop a rapid platform for monitoring bio/chemical interactions of bio macromolecules, e.g. DNA, using their mechanical properties. The MEMS device provided real-time monitoring of reaction dynamics without any surface or molecular modifications. A microfluidic device with a side opening was fabricated for the optimal performance of the MEMS device to operate at the air-liquid interface for performing bioassays in liquid while actuating/sensing in air. The minimal immersion of the MEMS device in the channel provided long-term measurement stability (>10 h). Importantly, the method allowed monitoring effects of multiple solutions on the same macromolecule bundle (demonstrated with DNA bundles) without compromising the reproducibility. We monitored two different types of effects on the mechanical responses of DNA bundles (stiffness and viscous losses) exposed to pH changes (2.1 to 4.8) and different Ag+ concentrations (1 μM to 0.1 M).

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“…The extremity of the molecule can, for example, be functionalized with biotin that can interact specifically with Streptavidin previously bound to a surface [4]. Here we briefly present a new application of the devices presented in [5][6][7][8][9][10][11] where no DNA modification is required. The method relies on the selfaggregation of DNA on teflonated super hydrophobic Silicon textured surface.…”

Section: Introductionmentioning

confidence: 99%

Stable Stretched Suspended DNA Molecules

Moretti¹,

Limongi²

2016

Adv Genet Eng

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Writing and Functionalisation of Suspended DNA Nanowires on Superhydrophobic Pillar Arrays

Cited by 31 publications

References 49 publications

Spontaneous formation of aligned DNA nanowires by capillarity-induced skin folding

Spontaneous formation of aligned DNA nanowires by capillarity-induced skin folding

A rapid and practical technique for real-time monitoring of biomolecular interactions using mechanical responses of macromolecules

Stable Stretched Suspended DNA Molecules

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