WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

Liu, Xi; Lan, Jie; Huang, Yunshuai; Cao, Penghui; Zhou, Chunlei; Ren, Yaken; He, Ning; Liu, Shijia; Tian, Yunlu; Nguyen, Thanhliem; Jiang, Ling; Wan, Jianmin

doi:10.1093/jxb/ery214

Cited by 79 publications

(70 citation statements)

References 41 publications

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“…White striped leaf phenotype was evident in the wsl5 mutant whereas z3 mutant exhibited altered phenotypes like transverse dark green/green sectors in mature leaf, shortened plant height, delayed flowering, and reduced panicle size in T 1 plants (Fig.4). These results confirm the findings of the original studies on Wsl5 and Z3 genes (Kim et al , 2018; Liu et al , 2018).…”

Section: Discussionsupporting

confidence: 91%

“…The homozygous wsl5 mutant is known to exhibit white-striped leaves in the seedlings (Liu et al , 2018). A single nucleotide polymorphism (T>C) is the causal mutation of wsl5 .…”

Section: Resultsmentioning

confidence: 99%

“…Wsl5 encodes a pentatricopeptide repeat protein and a single nucleotide polymorphism (T>C) in the first exon caused Leucine 151 to Proline 151 substitution resulting the mutant wsl5 . The wsl5 mutant displays white-striped leaves at the seedling stage (Liu et al , 2018). Zebra3 (Z3) is a putative citrate transporter gene and a single base substitution (T>C) in the third exon gives rise to a z3 mutant plant which exhibits dark-green/green variegation in mature leaves (Kim et al , 2018).…”

Section: Discussionmentioning

confidence: 99%

“…‘ Kitaake ’ ), a representative cereal crop and monocot model. OsWSL5 , a recently characterized rice gene, encodes a novel chloroplast-targeted pentatricopeptide repeat protein, which plays an essential role in rice chloroplast biogenesis (Liu et al , 2018). A single nucleotide polymorphism (T to C) located in the conserved region of exon 1 causes a leucine to proline amino acid substitution in the wsl5 mutant, which can be phenotypically visualized as longitudinal albino leaf striations.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous and precise generation of Zebra3 and Wsl5 mutations in rice using CRISPR/Cas9-mediated adenine base editors

Molla

Shih

Yang

2019

Preprint

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The CRISPR/Cas9-mediated base editing technology can efficiently generate point mutations in the genome without introducing double-strand break (DSB) or supplying a DNA donor template for homology-dependent repair (HDR). In this study, adenine base editors (ABEs) were used for rapid generation of precise point mutations in two distinct genes, OsWsl5, and OsZebra3 (Z3), in rice protoplasts and regenerated plants. The precisely engineered point mutations were stably inherited to subsequent generations. These single nucleotide alterations resulted in single amino acid changes and associated wsl5 and z3 phenotypes as evidenced by white stripe leaf and light green/dark green leaf pattern, respectively. Through selfing and segregation, transgene-free, base edited wsl5 and z3 mutants were readily obtained in a short period of time. We noticed a novel mutation (V540A) in Z3 locus could mimic the phenotype of Z3 mutation (S542P). Furthermore, we observed unexpected non-A/G or T/C mutations in the ABE editing window in a few of the edited plants. The ABE vectors and the method from in this study could be used to simultaneously generate point mutations in multiple genes in a single transformation and serve as a useful base editing tool for crop improvement as well as basic studies in plant biology.HighlightsAdenine base editors were adapted for plant base editing that can generate precise and heritable point mutations in rice genome without indel formation. The base editing approach allows rapid generation of transgene-free rice mutants with expected phenotypic changes.

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Section: Discussionsupporting

confidence: 91%

“…The homozygous wsl5 mutant is known to exhibit white-striped leaves in the seedlings (Liu et al , 2018). A single nucleotide polymorphism (T>C) is the causal mutation of wsl5 .…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous and precise generation of Zebra3 and Wsl5 mutations in rice using CRISPR/Cas9-mediated adenine base editors

Molla

Shih

Yang

2019

Preprint

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“…The chloroplast-localized OsSLA4 is mainly involved in the intron splicing of atpF, ndhA, petB, rpl2, rpl16, rps12 and trnG in chloroplasts (Wang et al 2018). The chloroplast-localized OsWSL5 is predominantly involved in the intron splicing of rpl2 and rps12 in chloroplasts (Liu et al 2018). The chloroplast-localized OsPGL12 is mainly involved in the intron splicing of ndhA in chloroplasts (Chen et al 2019).…”

Section: Introductionmentioning

confidence: 99%

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Shang

Chen

et al. 2020

Rice

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Background: The large family of pentatricopeptide repeat (PPR) proteins is widely distributed among land plants. Such proteins play vital roles in intron splicing, RNA editing, RNA processing, RNA stability and RNA translation. However, only a small number of PPR genes have been identified in rice. Results: In this study, we raised a mutant from tissue-culture-derived plants of Oryza sativa subsp. japonica 'Zhonghua 11', which exhibited a lethal chlorosis phenotype from germination to the third-leaf stage. The mutant was designated seedling-lethal chlorosis 1 (slc1). The slc1 mutant leaves showed extremely low contents of photosynthetic pigments and abnormal chloroplast development, and were severely defective in photosynthesis. Map-based cloning of OsSLC1 revealed that a single base (G) deletion was detected in the first exon of Os06g0710800 in the slc1 mutant, which caused a premature stop codon. Knockout and complementation experiments further confirmed that OsSLC1 is responsible for the seedling-lethal chlorosis phenotype in the slc1 mutant. OsSLC1 was preferentially expressed in green leaves, and encoded a chloroplast-localized PPR protein harboring 12 PPR motifs. Loss-of-function of OsSLC1 affected the intron splicing of multiple group II introns, and especially precluded the intron splicing of rps16, and resulted in significant increase in the transcript levels of 3 chloroplast ribosomal RNAs and 16 chloroplast development-related and photosynthesis-related genes, and in significant reduction in the transcript levels of 1 chloroplast ribosomal RNAs and 2 chloroplast development-related and photosynthesis-related genes. Conclusion: We characterized a novel chloroplast-localized PPR protein, OsSLC1, which plays a vital role in the intron splicing of multiple group II introns, especially the rps16 intron, and is essential for early chloroplast development and seedling survival in rice.

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Chloroplast Translation Machinery

Lokdarshi

2024

Nucleic Acids and Molecular Biology

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WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

Abstract: WSL5 is crucial for the splicing of the chloroplast genes rpl2 and rps12 under cold stress, and affects the retrograde signaling from plastids to the nucleus.

Cited by 79 publications

References 41 publications

Simultaneous and precise generation of Zebra3 and Wsl5 mutations in rice using CRISPR/Cas9-mediated adenine base editors

Simultaneous and precise generation of Zebra3 and Wsl5 mutations in rice using CRISPR/Cas9-mediated adenine base editors

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Chloroplast Translation Machinery

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