WT1 regulates HOXB9 gene expression in a bidirectional way

Schmidt, Viola; Sieckmann, Tobias; Kirschner, Karin M.; Scholz, Holger

doi:10.1016/j.bbagrm.2021.194764

Cited by 2 publications

(2 citation statements)

References 77 publications

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“…In this study, we studied the relationship between STC2 expression level and prognosis of HNSCC patients based on the TCGA database and found that high STC2 expression was associated with poor prognosis of HNSCC. HOXB9 is a HOX gene that, in addition to its key role in embryonic development, is also involved in the regulation of several human cancers [44]. Our nding that HOXB9 is highly expressed in HNSCC tissues with poor prognosis is consistent with the study by Darda et al, who demonstrated that HOXB9 expression is increased both in vitro and in HNSCC tissues [45].…”

Section: Discussionsupporting

confidence: 91%

A Novel Hypoxia-associated Gene Signature for Prognosis Prediction in Head and Neck Squamous Cell Carcinoma

Luo

Huang

et al. 2023

Preprint

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Background Head and neck squamous cell carcinoma (HNSCC) is the most common malignant tumor of head and neck, which seriously threatens human life and health. However, the mechanism of hypoxia-associated genes(HAGs)in HNSCC remains unelucidated. This study aims to establish a hypoxia-associated gene signature and the nomogram for predicting the prognosis of patients with HNSCC. Methods Previous literature reports provided a list of HAGs. The TCGA database provided genetic and clinical information on HNSCC patients. First, a hypoxia-associated gene risk model was constructed for predicting overall survival (OS) in HNSCC patients and externally validated in four GEO datasets (GSE27020, GSE41613, GSE42743, and GSE117973). Then, immune status and metabolic pathways were analyzed. A nomogram was constructed and assessed the predictive value. Finally, experimental validation of the core genes was performed by qRT-PCR and immunohistochemistry. Results A HNSCC prognostic model was constructed based on 8 HAGs. This risk model was validated in four external datasets and exhibited high predictive value in various clinical subgroups. Significant differences in immune cell infiltration levels and metabolic pathways were found between high and low risk subgroups. The nomogram was highly accurate for predicting OS in HNSCC patients. Conclusions The 8 hypoxia-associated gene signature can serve as novel independent prognostic indicators in HNSCC patients. The nomogram combining the risk score and clinical stage enhanced predictive performance in predicting OS compared to the risk model and clinical characteristics alone.

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Section: Discussionsupporting

confidence: 91%

A Novel Hypoxia-associated Gene Signature for Prognosis Prediction in Head and Neck Squamous Cell Carcinoma

Luo

Huang

et al. 2023

Preprint

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“…[ 38 ] In mNC subpopulations, we found that the Tcf21 + subpopulation preferentially expressed Wt1 and Wnt5a (Figure S7B , Supporting Information), which are required for anteroposterior axis specification. [ 39 ] GO enrichment analysis showed that the characteristic genes of the Tcf21 + subpopulation were associated with “anterior/posterior pattern specification,” “regulation of Wnt signaling pathway,” “segmentation,” “neural tube development,” and “neural tube closure” (Figure S7C , Supporting Information). Furthermore, gene set variation analysis (GSVA) showed that the Tcf21 + subpopulation was closely associated with “notochord morphogenesis” (Figure S7D , Supporting Information), the process in which the anatomical structures of the notochord are generated and organized (GO:0048570).…”

Section: Resultsmentioning

confidence: 99%

Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single‐Cell Resolution

et al. 2023

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The intervertebral disc (IVD) acts as a fibrocartilaginous joint to anchor adjacent vertebrae. Although several studies have demonstrated the cellular heterogeneity of adult mature IVDs, a single‐cell transcriptomic atlas mapping early IVD formation is still lacking. Here, the authors generate a spatiotemporal and single cell‐based transcriptomic atlas of human IVD formation at the embryonic stage and a comparative mouse transcript landscape. They identify two novel human notochord (NC)/nucleus pulposus (NP) clusters, SRY‐box transcription factor 10 (SOX10)+ and cathepsin K (CTSK)+, that are distributed in the early and late stages of IVD formation and they are validated by lineage tracing experiments in mice. Matrisome NC/NP clusters, T‐box transcription factor T (TBXT)+ and CTSK+, are responsible for the extracellular matrix homeostasis. The IVD atlas suggests that a subcluster of the vertebral chondrocyte subcluster might give rise to an inner annulus fibrosus of chondrogenic origin, while the fibroblastic outer annulus fibrosus preferentially expresseds transgelin and fibromodulin . Through analyzing intercellular crosstalk, the authors further find that notochordal secreted phosphoprotein 1 (SPP1) is a novel cue in the IVD microenvironment, and it is associated with IVD development and degeneration. In conclusion, the single‐cell transcriptomic atlas will be leveraged to develop preventative and regenerative strategies for IVD degeneration.

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WT1 regulates HOXB9 gene expression in a bidirectional way

Cited by 2 publications

References 77 publications

A Novel Hypoxia-associated Gene Signature for Prognosis Prediction in Head and Neck Squamous Cell Carcinoma

A Novel Hypoxia-associated Gene Signature for Prognosis Prediction in Head and Neck Squamous Cell Carcinoma

Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single‐Cell Resolution

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