X-irradiation of Balb/3T3: Sarcoma-forming Ability and Virus Induction

Pollock, Edward J.; Aaronson, Stuart A.; Todaro, George J.

doi:10.1080/09553007014550111

Cited by 15 publications

(2 citation statements)

References 10 publications

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“…One of these lines gives rise to revertants which no longer are morphologically altered and also no longer release MSV. The radiationtransformed A-31 subclone (R-4) has been described in detail elsewhere (Pollock et al, 1969). The two " spontaneously " transformed clones developed in one experiment where A-31 was co-cultivated with fibroblasts derived from a human sarcoma patient; however, the cells contain only mouse chromosomes and since this has not been reproduced we are uncertain as to the contribution, if any, of the human cells.…”

Section: Cell Linesmentioning

confidence: 99%

Altered glucose transport kinetics in murine sarcoma virus‐transformed BALB/3T3 clones

Hatanaka¹,

Todaro²,

Gilden³

1970

Intl Journal of Cancer

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The kinetics of glucose transport were determined for a number of spontaneous and virus‐transformed cell lines derived from a single clone of BALB/3T3 cells. SV40‐transformed and mouse leukemia virus‐infected cells were characterized by a Km very similar to that of the parental cell line. In contrast, murine sarcoma virus‐transformed cells had a much lower Km. These findings are in agreement with previous results obtained with mass cultures of NIH Swiss mouse embryo cells. One X‐irradiation and two spontaneous transformants also showed low Km values and no virions or virion antigens. These latter cell lines should provide an opportunity for a crucial test of the relationship between glucose transport kinetics and a functional sarcoma virus genome.

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Section: Cell Linesmentioning

confidence: 99%

Altered glucose transport kinetics in murine sarcoma virus‐transformed BALB/3T3 clones

Hatanaka¹,

Todaro²,

Gilden³

1970

Intl Journal of Cancer

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“…is a subclone of A31 transformed in vitro by methylcholanthrene (personal communication). R4 is a survivor of 1,200 rads of X irradiation which has the transformed phenotype (30). SVT2 is A31 transformed by SV40 virus (2).…”

Section: Methodsmentioning

confidence: 99%

Partial Transcription of Murine Type C Viral Genomes in BALB/c Cell Lines

Benveniste

Todaro

Scolnick

et al. 1973

J Virol

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The mouse cell line, BALB/c 3T3, and its derivatives transformed either spontaneously or by treatment with a variety of external agents, were analyzed for cytoplasmic RNA complementary to DNA products prepared from the Kirsten strain of murine sarcoma-leukemia virus, and from an endogenous type C virus of BALB/c 3T3. Although none of these cell lines spontaneously releases complete type C virions, they all contain RNA which is partially homologous to a portion of the 35S RNA isolated from these viruses. The parental cell line, BALB/c 3T3, contains a low level of viral-related RNA, and there is an increased amount of this RNA in some of the transformed cells. The RNA detected represents only a fraction of the viral RNA found in virus-producing cells. The formation of RNA: DNA hybrids was detected by equilibrium centrifugation in Cs2SO, density gradients and by analysis with a single-strand-specific nuclease from Aspergillus oryzae. Viral DNA products prepared either from an endogenous reaction with whole virus in the presence of actinomycin D or from purified 70S viral RNA as template using avian myeloblastosis virus DNA polymerase yield comparable data. In addition, all of the BALB/c lines examined produce detectable levels of murine type C virus group-specific antigen.

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