X-ray diffraction and Fourier transform infrared spectroscopic analyses of wood blocks decayed by wood rotting fungi classified into Polyporales

Abstract

Comparative genomics on wood rotting fungi suggested that the evolution from ancestral white-rot fungi to brown-rot fungi, which are at least phylogenetically classified into the four orders of Boletales, Dacrymycetes, Gloeophyllales, and Polyporales, occurred independently multiple times. In the present study, three brown-rot fungi (Fomitopsis palustris, F. pinicola, and Wolfiporia cocos) and a white-rot fungus (Trametes versicolor) were used to investigate wood decay manner in Polyporales. Wood (Cryptomeria japonica) blocks decayed by the fungi above were analyzed by X-ray diffraction and Fourier transform infrared spectroscopy analyses. As a result, cellulose crystallinities and infrared spectra were significantly different between white-rot and brown-rot fungi. Multivariate analysis of the infrared spectroscopy data showed the somewhat different plot patterns between brown-rot species.