X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood

Abstract

Using quantitative real-time polymerase chain reaction (PCR) array, we explored and compared the expression changes of inflammation-related genes in human peripheral blood irradiated with 0.5, 3, and 10 Gy doses of X-rays 24 h after exposure. Results indicated that the expression of 62 out of 84 genes was significantly altered after X-ray radiation. Among these 62 genes, 35 (such as TNFSF4) are known to be associated with radiation response, but others are novel. At a low radiation dose (0.5 Gy), 9 genes were up-regulated and 19 were down-regulated. With further increased dose to 3 Gy, 8 unique genes were up-regulated and 19 genes were down-regulated. We also identified 48 different genes that were differentially expressed significantly after 10 Gy of irradiation, and among these transcripts, up-regulated genes accounted for only one-third (16 genes) of the total. Of the 62 genes, 31 were significantly altered only at a specific dose, and a total of 10 genes were significantly expressed at all 3 doses. The dose- and time-dependent expression of CCL2 was confirmed by quantitative real-time reverse-transcription PCR. A number of candidate genes reported herein may be useful molecular biomarkers of radiation exposure in human peripheral blood.

Aloe vera modulates X-ray induced hematological and splenic tissue damage in mice

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Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells

“…As a highly sensitive and reliable method of gene expression profiling, qPCR array combines qRT-PCR and gene chips which can screen differentially expressed genes based on mRNA. qPCR array is a useful tool for studies on signaling pathway [ 27 , 28 ]. In agreement with these findings, in the current study, the results demonstrated that 44 genes (including MyD88-dependent and nondependent TLR4, NLRS, MARK, and NF- κ B) were upregulated after LPS stimulation.…”

How Macrophages Become Transcriptionally Dysregulated: A Hidden Impact of Antitumor Therapy

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