X‐ray Microanalysis in Cell Biology and Cell Pathology

Roomans, G. M.; Euler, Anne von

doi:10.1006/cbir.1996.0014

Cited by 27 publications

(20 citation statements)

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“…Electron probe X-ray microanalysis has been used for more than 30 years to obtain information regarding the distribution of elements in cells and tissues. This technique can simultaneously detect any elements heavier than C (or Na depending on the detector used) with a resolution limit of about 40 nm and sensitivity of 10 ¡18 g (Roomans and Von Euler, 1996). However, electrons are strongly scattered within thick specimens, resulting in interaction volumes of up to 10 m and thus much lower resolution (Roomans, 1988).…”

Section: Introductionmentioning

confidence: 99%

Correlative microXRF and optical immunofluorescence microscopy of adherent cells labeled with ultrasmall gold particles

McRae

Lai

Vogt

et al. 2006

Journal of Structural Biology

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Section: Introductionmentioning

confidence: 99%

Correlative microXRF and optical immunofluorescence microscopy of adherent cells labeled with ultrasmall gold particles

McRae

Lai

Vogt

et al. 2006

Journal of Structural Biology

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“…Electron microprobe (EM) uses an energy dispersive detector with a transmission electron microscopy (Roomans 1988;Roomans and Von Euler 1996). In this method, an electron beam is used to excite outer shell electron of atoms which upon re-arrangement emits X-rays.…”

Section: Methods To Image Trace Metals In Biological Materials With Spmentioning

confidence: 99%

Quantitative imaging of metals in tissues

Ralle

Lutsenko

2009

Biometals

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Metals and other trace elements play an important role in many physiological processes in all biological systems. Characterization of precise metal concentrations, their spatial distribution, and chemical speciation in individual cells and cell compartments will provide much needed information to explore the metallome in health and disease. Synchrotron-based X-ray fluorescent microscopy (SXRF) is the ideal tool to quantitatively measure trace elements with high sensitivity at high resolution. SXRF is based on the intrinsic fluorescent properties of each element and is therefore element specific. Recent advances in synchrotron technology and optimization of sample preparation have made it possible to image metals in mammalian tissue with submicron resolution. In combination with correlative methods, SXRF can now, for example, determine the amount and oxidation state of trace elements in intra-cellular compartments and identify cell-specific changes in the metal ion content during development or disease progression.

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“…DNA oligonucleotides with four runs of An alternative view, which is proposed here, is that altered K + /Na + ratios in cancer cells may result in altered G-quadruplex DNA structure and function. A number of reports have documented altered K + /Na + ratios in tumor cells [77], although the magnitude and trend of change appear to be tumor type specific [78]. These cancer cell-specific K + /Na + ratios may effect conformational changes in G-quadruplex structures, resulting in altered transcription of particular genes and altered telomere function and maintenance.…”

Section: G-quadruplex Dna Metal Ion Bindingmentioning

confidence: 99%

G-Quadruplex DNA as a Target for Drug Design

Kerwin¹

2000

CPD

225

110

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Telomeres are structures on the ends of chromosomes that are required for chromosomal stability. Telomeric DNA contains a single-stranded G-rich DNA overhang, which may adopt a G-quadruplex structure. Telomere shortening has been implicated in cellular senescence. Telomerase is an enzyme which synthesizes the G-rich strand of telomere DNA. Telomerase activity is highly correlated with cancer and may allow cancer cells to escape senescence. Based on these observations, telomerase has been proposed as a potential target for anticancer drug design. The targeting of telomerase is associated with potential problems, including the existence in some cancer cells of telomerase-independent mechanisms for telomere maintenance, and the long delay time between telomerase inhibition and effects on proliferation. One promising approach for inhibiting telomerase involves targeting the G-quadruplex DNA structures thought to be involved in telomere and telomerase function. Compounds that specifically bind G-quadruplex DNA may interact directly with telomeres, in addition to inhibiting telomerase, and produce more immediate antiproliferative effects. The diamidoanthraquinones, porphyrins, and perylene diimides have all been shown to bind G-quadruplex DNA and inhibit telomerase. Most of these compounds also bind double-stranded DNA and are cytotoxic at the concentrations required to inhibit telomerase; however, certain perylene diimides appear to be non-cytotoxic, G-quadruplex selective telomerase inhibitors. Biological characterization of such compounds may provide validation for the concept of the G-quadruplex as a target in drug design.

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X‐ray Microanalysis in Cell Biology and Cell Pathology

Cited by 27 publications

References 0 publications

Correlative microXRF and optical immunofluorescence microscopy of adherent cells labeled with ultrasmall gold particles

Correlative microXRF and optical immunofluorescence microscopy of adherent cells labeled with ultrasmall gold particles

Quantitative imaging of metals in tissues

G-Quadruplex DNA as a Target for Drug Design

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