X-Ray Microscopy for Detection of Metals in the Brain

Collingwood, Joanna F.; Adams, F.

doi:10.1007/978-1-4939-6918-0_2

Metals in the Brain

2017

DOI: 10.1007/978-1-4939-6918-0_2

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X-Ray Microscopy for Detection of Metals in the Brain

Joanna F. Collingwood

F. Adams

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2020

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Cited by 2 publications

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“…Among the analytical approaches developed to investigate physiological heterogeneities of trace elements in brains 24,25 , synchrotron radiation-induced Xray fluorescence (XRF hereafter) is part of the few imaging approaches 26,27 , the only non-destructive multi-elemental method of high sensitivity. XRF has been used to analyze Fe traces in brains affected by Parkinson's disease 28 , showing that this element is heterogeneously distributed in brain tissues and accumulates in both the neuronal cells and the extra-neuronal substantia nigra tissue of Parkinson's patients. It reaches levels three times higher than in non-affected subjects [29][30][31] .…”

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Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease

et al. 2020

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Sub-cellular trace element quantifications of nano-heterogeneities in brain tissues offer unprecedented ways to explore at elemental level the interplay between cellular compartments in neurodegenerative pathologies. We designed a quasi-correlative method for analytical nanoimaging of the substantia nigra, based on transmission electron microscopy and synchrotron X-ray fluorescence. It combines ultrastructural identifications of cellular compartments and trace element nanoimaging near detection limits, for increased signal-to-noise ratios. Elemental composition of different organelles is compared to cytoplasmic and nuclear compartments in dopaminergic neurons of rat substantia nigra. They exhibit 150-460 ppm of Fe, with P/Zn/Fe-rich nucleoli in a P/S-depleted nuclear matrix and Ca-rich rough endoplasmic reticula. Cytoplasm analysis displays sub-micron Fe/S-rich granules, including lipofuscin. Following AAV-mediated overexpression of α-synuclein protein associated with Parkinson's disease, these granules shift towards higher Fe concentrations. This effect advocates for metal (Fe) dyshomeostasis in discrete cytoplasmic regions, illustrating the use of this method to explore neuronal dysfunction in brain diseases.

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Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease

et al. 2020

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Hybrid organic-inorganic Fe₃O(TFBDC)₃(H₂O)₃·(DMF)₃ compound synthesized by slow evaporation method: Characterization and comparison of magnetic properties

Laurikėnas¹,

Mažeika²,

Baltrūnas³

et al. 2020

physics

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In this study for the synthesis of a hybrid organic-inorganic Fe3O(TFBDC)3(H2O)3·(DMF)3 compound a slow evaporation method has been suggested. THe synthesis product was characterized using X-ray powder diffraction (XRD) analysis, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) coupled with SEM and electron paramagnetic resonance (EPR) spectroscopy. THe antiferromagnetic/weakly ferromagnetic behaviour of the synthesized sample was confirmed by magnetization measurements and Mössbauer spectroscopy. THe synthesized magnetic material could be itself tested for different medical applications and could be used as precursor material for the preparation of nanostructured iron oxides with a variety of useful properties for biomedicine.

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X-Ray Microscopy for Detection of Metals in the Brain

Cited by 2 publications

References 54 publications

Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease

Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease

Hybrid organic-inorganic Fe₃O(TFBDC)₃(H₂O)₃·(DMF)₃ compound synthesized by slow evaporation method: Characterization and comparison of magnetic properties

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X-Ray Microscopy for Detection of Metals in the Brain

Cited by 2 publications

References 54 publications

Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease

Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease

Hybrid organic-inorganic Fe3O(TFBDC)3(H2O)3·(DMF)3 compound synthesized by slow evaporation method: Characterization and comparison of magnetic properties

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Hybrid organic-inorganic Fe₃O(TFBDC)₃(H₂O)₃·(DMF)₃ compound synthesized by slow evaporation method: Characterization and comparison of magnetic properties