X-ray studies on changes in embryo and endosperm morphology during priming and imbibition of tomato seeds

Liu, Y.; Burg, W.J. van der; Aartse, J.W.; Zwol, R. A. van; Jalink, H.; Bino, R.J.

doi:10.1017/s0960258500001756

Cited by 40 publications

(32 citation statements)

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“…In studying internal seed morphology, one parameter which may be evaluated is embryo size, which can be defined by the degree of development and the free space in the internal seed cavity ; this free space was determined for the first time for tomato seeds and includes the free spaces between the embryo and the endosperm or between the seed contents and the tegument, which can occur in different parts of the seed, but normally around the embryo (Liu et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Relationship between eggplant seed morphology and germination

Silva¹,

Cícero²,

Bennett

2012

Rev. bras. sementes

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-Structural differences such as abnormalities, damage and free spaces in seeds may affect germination. The aim of this study was to study the relationship between eggplant seed morphology and seed germination. Ten seed lots of the eggplant cultivar Embu were evaluated by X-ray image analysis and the germination test. Seed image analysis was performed by Image Pro Plus ® software and the whole seed area and free space between the embryo and endosperm were measured. The internal seed area filled by the embryo and endosperm was calculated from the difference between the whole seed and free space areas. Based on these results and visual seed analysis, seeds were classified into three categories and information on germination was obtained for each one. X-ray image analysis provides a perfect view of the internal seed parts and for seed morphology studies. An increase in seed area filled by the endosperm and embryo does not improve seed germination. Mechanical seed damage and deteriorated tissues can adversely affect seed germination.Index terms: X-ray, Solanum melongena L., embryo.Relação entre a morfologia interna e a germinação de sementes de berinjela RESUMO -Diferenças estruturais como anormalidades, danos e áreas livres no interior da semente podem afetar diretamente a germinação. O objetivo deste trabalho foi verificar a relação existente entre a morfologia interna de sementes de berinjela e a germinação. Para tanto, sementes de berinjela, representadas por 10 lotes (cultivar Embu), foram avaliadas por meio do teste de raios X, sendo posteriormente submetidas ao teste de germinação. As imagens das sementes foram analisadas com o software Image Pro Plus ® e o espaço livre entre o embrião e o endosperma de cada semente foi mensurado calculando-se o espaço da cavidade interna das sementes ocupado pelo embrião e endosperma, por diferença; com base nestes dados e na análise visual das sementes, foi realizada a classificação das sementes em três categorias e os resultados foram confrontados com os dados de germinação. Conclui-se que a análise de raios X permite a visualização das partes componentes das sementes de berinjela e, assim, possibilita o estudo da relação entre a morfologia interna e a germinação. A presença de maior área ocupada pelo embrião e endosperma não favoreceu a germinação. Danos mecânicos ou presença de tecidos deteriorados nas sementes podem afetar negativamente a germinação.Termos para indexação: Solanum melongena L., raios X, embrião.

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Section: Introductionmentioning

confidence: 99%

Relationship between eggplant seed morphology and germination

Silva¹,

Cícero²,

Bennett

2012

Rev. bras. sementes

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“…Reid et al (1991), desenvolvendo sistema de visão computadorizado para detecção de danos mecânicos em grãos de milho, concluíram que o método eletrônico foi superior ao convencional. Por outro lado, a imagem digital tem sido utilizada para avaliar características externas de sementes, de bulbos e de tubérculos (Van Der Vooren et al, 1992;Van Der Vooren & Van Der Heijden, 1993), enquanto a imagem digital de raios-X foi introduzida para avaliar as morfologias do embrião e do endosperma de sementes de Arabidopsis thaliana e de tomate (Liu et al, 1993;Bino et al, 1993;Van Der Burg et al, 1994).…”

Section: Introductionunclassified

Avaliação do relacionamento entre danos mecânicos e vigor, em sementes de milho, por meio da análise de imagens

Cícero

Banzatto

2003

Rev. bras. sementes

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RESUMO -A possibilidade de utilização da técnica de análise de imagens, para a determinação de danos mecânicos em sementes, é promissora; é um método de precisão, onde as sementes podem ser examinadas individualmente em imagens ampliadas e capazes de indicar, com detalhes, a área danificada, a localização e a extensão do dano. Por se tratar de método não destrutivo, as sementes em análise podem ser submetidas a testes fisiológicos e, desta forma, permitir o estabelecimento de relações entre os danos mecânicos e os prejuízos causados à qualidade. Assim, o presente trabalho teve o objetivo de estudar os efeitos dos danos mecânicos sobre o vigor de sementes de milho, utilizando a referida técnica. Cinqüenta sementes de cada um dos cultivares estudados (CO 32, AG 6690 e Attack) foram selecionadas visualmente, de tal maneira que a amostra fosse constituída por sementes íntegras e com danos mecânicos de intensidades variáveis. Em seguida, as sementes foram radiografadas, fotografadas (faces ventral e dorsal) e destinadas ao teste de frio (método do rolo de papel com terra). As plântulas normais, anormais e sementes mortas provenientes do teste de frio foram, também, fotografadas. As imagens obtidas foram disponibilizadas em computador de tal maneira que, examinadas simultaneamente na tela do monitor, pudessem ser buscadas relações de causa e efeito nas imagens anteriores e posteriores ao teste de frio. Os resultados obtidos indicam que o método estudado permite relacionar o dano mecânico com eventuais prejuízos ocasionados ao vigor das sementes de milho.Termos para indexação: Zea mays, imagem digital, raios-X. EVALUATION OF IMAGE ANALYSIS IN DETERMINING THE RELATIONSHIP BETWEEN MECHANICAL DAMAGE AND SEED VIGOR IN MAIZEABSTRACT -Image analysis is a very promising technique to determine mechanical damage in seeds. This precise method examines seeds individually using enlarged images in which damaged areas as well as their exact location and extension can be found and examined in detail. Since this is a non-destructive method, analyzed seeds can be submitted to physiological tests to establish the relationship between mechanical damage and quality loss. The objective of the present investigation was to study the effects of mechanical damage on corn/maize seed vigor using image analysis. Fifty seeds from CO 32, AG 6690 and Attack cultivars were visually selected to form a sample of whole seeds with varying degrees of mechanical damage. The seeds were x-rayed, photographed (ventral and dorsal sides) and submitted to a cold test. Photographs were repeated after the cold test. Images were transferred to a computer so that pre and post cold test images could be examined simultaneously on the monitor to determine the possible relationship between cause and effect. Results indicated that the method under study permits association of mechanical damage with eventual losses caused to corn/maize seed vigor.Index terms: Zea mays L., digital image, X-rays. INTRODUÇÃOA importância de danos mecânicos em sementes de milho, sobre a emerg...

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“…This test has been used to study seed internal morphology, such as in the pumpkin (Cucurbita moschata) (Carvalho et al, 2009), pepper (Capsicum annuum cv. Sigaretta biondo) (Dell'Aquila, 2007) and tomato (Solanum lycopersicum) (Liu et al, 1993). The objective of the present study was to establish a relationship between mericarps color and internal morphology of parsley with the germination, using sorting by coloration and the X-ray test.…”

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confidence: 97%

Germination of parsley seeds influenced by mericarps color and internal morphology

et al. 2013

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The objective of the present study was to establish a relationship between mericarps color and internal morphology of parsley with the germination, using sorting by coloration and the X-ray test. The completely randomized design was used, with four replications per treatment. The treatments were constituted of four lots (A, B, C and D) and three mericarps colors (green, yellow and black) in 4×3 factorial experiment. The mericarps were exposed to X-ray test (60s; 20kV), being used the digital equipment Faxitron X-ray, model MX-20 DC12 and then, the irradiated mericarps were submitted to the germination test. The yellow mericarps showed higher germination and speed germination index. The green mericarps showed deformations and impaired formation of embryo, while black and yellow mericarps showed up without deformation and with a well-formed embryo. We concluded that there is a relationship between parsley seed germination and mericarps color. Poorly formed embryos and misshapened seed coats are related to green mericarps.

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X-ray studies on changes in embryo and endosperm morphology during priming and imbibition of tomato seeds

Cited by 40 publications

References 14 publications

Relationship between eggplant seed morphology and germination

Relationship between eggplant seed morphology and germination

Avaliação do relacionamento entre danos mecânicos e vigor, em sementes de milho, por meio da análise de imagens

Germination of parsley seeds influenced by mericarps color and internal morphology

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