X-STR typing for an identification casework

Barbaro, A.; Cormaci, P.

doi:10.1016/j.ics.2005.09.035

Cited by 11 publications

(9 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first commercialized X-STRs kit was Mentype® Argus X-UL PCR amplification kit (BioType AG) that co-amplified four X-STRs markers namely DXS8378, HPRTB, DXS7423 and DXS7132 plus Amelogenin in a single dye channel [40,41]. Subsequently, other four X-STRs (DXS10134, DXS10074, DXS10101, and DXS10135) were included into the improved Mentype® Argus X-8 kit [42].…”

Section: Development Of X-strs Analysismentioning

confidence: 99%

Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review

Mahat

Alwi²,

Salleh³

et al. 2023

J.Trop.Life.Science

View full text Add to dashboard Cite

The forensic DNA profiling technique has tremendously contributed to forensic human identification, an important aspect in forensic investigations. In instances whereby comparison samples are unavailable, utilization of short tandem repeats of X chromosome (X-STRs) may prove useful to resolve complex kinship investigations involving missing persons and mass disasters. Despite such evidential values, the use of X-STRs during investigations remains scarce in many Southeast Asian countries including Malaysia, requiring concerted efforts for establishing forensic statistical support for its diverse populations (especially the admixture populations), standardizing core loci and procedure, improving the knowledge among practitioners as well as developing suitable standard operating procedure for incorporating X-STRs analysis in the overall DNA profiling framework. Hence, this review paper aims to highlight the developments, applications and population data of X-STRs, as well as its challenges and future insights for forensic casework.

show abstract

Section: Development Of X-strs Analysismentioning

confidence: 99%

Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review

Mahat

Alwi²,

Salleh³

et al. 2023

J.Trop.Life.Science

View full text Add to dashboard Cite

show abstract

“…They have shown great forensic ability and are, therefore, recognized as efficient tools in forensic investigations of kinship cases [2][3][4]. A variety of X-STR systems have been developed and added to the list of conventional markers for better results in complex kinship analysis and cases involving mother-son and father/daughter relationship [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and haplotypic structure of a Saudi population sample using Investigator Argus X-12 amplification kit

Messaoudi

Babu

Alsaleh

et al. 2019

Preprint

View full text Add to dashboard Cite

Background:X-chromosome short tandem repeat (X-STR) markers have shown a great capability in forensic identity investigations and paternity testing involving kinship analysis. Material and methods:In the current study, the distribution of 12 X-STR loci (DXS10148, and HPRTB) located in four linkage groups (LG)s was evaluated using Investigator ® Argus X-12 Amplification Kit in 200 unrelated healthy individuals (105 males, and 95 females) from the central region of the Saudi Arabia in order to create a DNA database. Results:No significant difference was recorded in the allele frequencies of males and females.Our results indicated that DXS10146 locus was the most informative with 21 alleles while DXS8378 locus was the least with 5 alleles. Hardy-Weinberg equilibrium (HWE) was applied and confirmed for all loci in the female samples, except for DXS10074, DXS10101, DXS10135 and DXS10148 (p > 0.05/12 = 0.0042). Forensic parameters showed that all X-STRs loci either as individual markers or as linkage groups provide genetic information with high discrimination that is appropriate for forensic purposes with Paternity Informed Consent (PIC), Power of exclusion (PE), and Paternity index (PI) varied from 0.61211 to 0.917979, 0.38722 to 0.842949, and 0.038416 to 0.16367, respectively. A significant Linkage disequilibrium (LD) with p-value after Bonferroni correction p ≤ 0.05/66= 0.0008 was observed for 17 pairs of loci in male samples and 4 pairs of loci in female. In the male group, LG3 showed relatively high values of Haplotype diversity (HD). This indicated that these LGs were quite informative in the studied Saudi group and would have high application value in forensic sciences. The pairwise genetic distance fixation index (Fst) results showed that the Saudi population is genetically close to the Egyptian and Emirati populations and distant to the Turkish population. Genetic distances were represented in a non-metric MDS plot representing that Saudis cluster with Middle East populations and are clearly separated from European and East Asian populations. Conclusion:The current study revealed that Investigator® Argus 12 X-STR kit would support forensic application, kinship testing involving female offspring, and human identification in Saudi populations.

show abstract

“…X-STRs have also advantage over autosomal STRs for paternity cases involving close blood relatives as alternative putative fathers and in deficiency paternity cases, i.e. when the DNA sample from putative father is not available and DNA from paternal relative has to be analyzed instead [17]. Further, X-linked STRs can be used to solve sibship status, without using father's DNA, of two females having the same biological father [18,19].…”

Section: Introductionmentioning

confidence: 99%

Scope of X-Chromosomal MiniSTRs: Current Developments

Israr¹

2014

J Forensic

View full text Add to dashboard Cite

The experience of using autosomal short-length amplicon STRs or miniSTRs in profiling of degraded DNA and mass disaster victims is extended into the realm of X-chromosomal (ChrX) STR miniaturization. About half of the total X-STRs are now short-length amplicons and the focus is shifting to using the mini versions of all of them. Joint multiplexing of these loci can be used for solving complex paternity cases and association of mass disaster victims with their families. This technology may herald a new dimension for research in population genetics and evolution. We present an overview of the progress made thus far and the future scope and prospects for X-miniSTRs.

show abstract

X-STR typing for an identification casework

Cited by 11 publications

References 4 publications

Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review

Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review

Genetic diversity and haplotypic structure of a Saudi population sample using Investigator Argus X-12 amplification kit

Scope of X-Chromosomal MiniSTRs: Current Developments

Contact Info

Product

Resources

About