Xanthine oxidase activity regulates human embryonic brain cells growth

Danielyan, Kristine Edgar; Kevorkian, G. A.

doi:10.7124/bc.000121

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Our previous results [2] indicated that the early inhibition of the XO in the human brain derived cell culture utilizing allopurinol were initiating the increase in the number of the cells in comparison with the later stage of inhibition.…”

Section: Assesment Of Xo Activity By the Estimation Of Uric Acid Formmentioning

confidence: 99%

“…Their addition into the primary cultured neurons of various brain regions from fetal rat brain significantly promoted the neuronal survival in high cell density culture (10(5) cells/cm (2), but showed no positive effects in low density cell culture (5 x 10(3) cells/cm2). This neurotrophic effect of PLP was remarkably suppressed by picrotoxin and Ifenprodil.…”

Section: Introductionmentioning

confidence: 99%

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Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

Danielyan¹

2013

AJBR

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The work is focused on the role of single components of Vitamin B complex: pyridoxine, riboflavin, thiamine, and nicotinamide in the processes of growth and development of the human brain derived cells. We, also, have assayed the activity of Xanthine Oxidase in the presence of above mentioned subcomponents to delineate the possible mechanism of their action. Results indicate that the all components of Vitamin B complex might be responsible for cells' growth, maturation, proliferation. During early period of the cells' growth the most important components were thiamine and Pyridoxine, initiating cells' proliferation (number of the cells in one field: 2556, 17±355, 68, 2179,0±223,55, resp) vs control (1562,94±146,45), whereas during the late stages of maturation the most important components responsible for differentiation, were riboflavin and nicotinamide (3774.77± 188.41, 3558.82±152.90 resp. vs control 2905±263.75; p<0.035). In comparison with the all other subcomponents of Vitamin B complex only in pyridoxine containing samples, XO activity was specifically inhibited by allopurinol (percentile of inhibition 160,00±60,00 vs control 31,03±6,92, p<0,05). We have concluded that Pyridoxine might interact with XO and regulate its activity. All components of Vitamin B complex are able to initiate cells development and growth; however, they are supposed to be selectively utilized in time dependent manner to guarantee the highest efficiency of the cells development, maturation and proliferation

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Section: Assesment Of Xo Activity By the Estimation Of Uric Acid Formmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

Danielyan¹

2013

AJBR

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“…Our previous experiments were proving that the low concentration of allopurinol utilized from day first to 12 increased the cells number in the cell culture. The experiments were designed for the estimation of the endogenous XO activity [4]. Our current data demonstrate that even exogenous addition of xanthine, NAD + and allopurinol does promote increase of the cells' number.…”

Section: Discussionmentioning

confidence: 61%

Xanthine Dehydrogenase Inhibition Stimulates Growth and Development of Human Brain Derived Cells

Danielyan¹,

Chailyan²

2013

AJMBR

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Background: Reactive Oxygen Species (ROS) play a critical role in development of number pathologies. Xanthine Oxidase (XO) as well as the Xanthine Dehydrogenase (XDH) are two enzymes responsible for the last steps of purine catabolism, hydroxylation of a wide variety pyrimidines, and the initiation of ROS synthesis. In our current experiments we have analyzed whether exogenously added allopurinol, not only the inhibitor of XO but also XDH, is capable for in vitro human brain derived cells growth, development and proliferation. Methods: We have used described by Mark Mattson's neuronal cell culturing technique to seed and keep cells in vitro over 12 days. The death of the cells was visualized by the staining with Trypan Blue. Pixcavator and Image Tool programs served for the calculation of the cells' number and size. Results: In comparison with the control group exogenously added xanthine as well as NAD + or addition of NAD + along with xanthine didn't play any critical role. Only the group, treated with NAD + , xanthine and allopurinol promoted elevation of the cells' number in the statistically significant way on the day 12 th. Number of the death cells in comparison with the control groups in the cells groups treated with the xanthine, NAD + , as well as allopurinol was less, whereas in the NAD + and xanthine treated group this number was higher than in the control group. Conclusion: We have concluded, that treatment with the low concentration of allopurinol, will guarantee the survival of the cells, decrease the number of the death cells and promote the proliferative processes. These conclusions are similar for allopurinol treatment in condition of exogenously stimulated activity of XO/XDH as well as endogenously stimulated activity of these enzymes.

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“…In our previous experiments, we have proved experimentally, by the inhibition of the Xanthine Oxidoreductase (EC 1.17.3.2), the one of the last and the key regulative enzymes in the chain of the purines catabolism-responsible enzymes, by its classical compound-Allopurinol, it is possible to decrease the speed of purines decay [1] [2]. In addition, we have proved, by impacting the XOR, it is possible to accelerate the cells proliferation or have an influence on the cells' deaths [2] [3] [4].…”

Section: Introductionmentioning

confidence: 99%

Purine and Pyrimidine-Linked Enzymes and Genes are Strongly Responsible for the Development of Tumors, Particularly Glioblastoma Multiforme

Danielyan¹,

Yeghiazaryan²,

Chailyan³

et al. 2020

CMB

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We were aiming to delineate, by the utility of the biological data results, in our investigations the link between the purine and pyrimidine metabolism and development of the glioblastoma. We analyzed the sets of the genes, belonging to the purine and pyrimidine metabolism by the utility of GSEA software as well as MSIgnDB application of the GSEA. The GEO database, GEOR2 tools were serving for the visualization of the genes expression profiles of the disease. The Cancer Proteome Atlas as well as the tools of the data sets were also used to collect and analyze the results. We concluded and came to the following consequential results. 1) Neurogenesis and Glioblastoma are sharing some common genes. 2) Purine and pyrimidine metabolism-linked enzymes and genes are responsible for the upregulation of DNA and mRNA synthesis in the settings of the tumor development. 3) EGFR expression responsible genes, mRNA as well as protein is upregulated during the development of the glioblastoma. 4) GMPS genes are more strongly upregulated in the settings of the glioblastoma than ADSL. 5) PRPS1 is strongly synthetized in neurospheres in contrast to the mature tissue during glioblastoma development.

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Xanthine oxidase activity regulates human embryonic brain cells growth

Cited by 5 publications

References 12 publications

Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

Xanthine Dehydrogenase Inhibition Stimulates Growth and Development of Human Brain Derived Cells

Purine and Pyrimidine-Linked Enzymes and Genes are Strongly Responsible for the Development of Tumors, Particularly Glioblastoma Multiforme

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