Xanthone compounds in shoot cultures of Gentianella bulgarica

Abstract

Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l -1 ? NAA 0.1 mg l -1 . Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC 50 = 0.26 mg ml -1 ), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC 50 from 1.6 to 4.4 mg ml -1 ).

Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula

“…Huo and Zheng (2002) reported shoot regeneration in tissue cultures of G. albifora, while shoot cultures of European G. austriaca and G. bulgarica have been established recently (Vinterhalter et al 2008;Janković et al 2011). In contrast, in vitro propagation of species from the genera Gentiana, Swertia, Centaurium, and Blackstonia has been well documented (Skrzypczak et al 1993;Hosokawa et al 1996;Momčilović et al 1997;Vinterhalter and Vinterhalter et al 1998;Chaudhuri et al 2007;Subotic et al 2006;Bijelović et al 2004).…”

The Gentianaceae - Volume 2: Biotechnology and Applications

“…This chapter describes regeneration systems employed for the multiplication of a particular species or cultivar. formosana (Chueh et al 2001), and Gentianella bulgarica (Janković et al 2011). The experimental targets in publication have been to increase gentiopicrosides and xanthones and to produce a new spectrum of their analogs.…”

“…The experimental targets in publication have been to increase gentiopicrosides and xanthones and to produce a new spectrum of their analogs. Conversely, the concentrations of bellidifolin-O-glucoside (GBL) and demethylbellidifolin (DMB) were lower in the shoot culture than in Gentianella bulgarica plants growing naturally outdoors (Janković et al 2011). In order to describe the effect of culture on cultured explants, the schema of experiments employed: culture initiation, material multiplication (shoot regeneration or somatic embryo production), plantlet regeneration, and the assessment of secondary metabolites.…”

“…Huo and Zheng (2002) reported shoot regeneration in tissue cultures of G. albifora, while shoot cultures of European G. austriaca and G. bulgarica have been established recently (Vinterhalter et al 2008;Janković et al 2011). Huo and Zheng (2002) reported shoot regeneration in tissue cultures of G. albifora, while shoot cultures of European G. austriaca and G. bulgarica have been established recently (Vinterhalter et al 2008;Janković et al 2011).…”

Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production

“…and Swertia spp. However, these studies were performed in only a few species of the genus Gentianella , and included G. austriaca [ 18 ], G. bulgarica [ 19 , 20 ], Gentianella albifpra [ 21 ], and G. bicolor [ 22 ]. Considering the rarity and medicinal importance of G. lutescens, tissue culture would be a suitable tool, not only for conservation purposes, but also for overcoming the deficit of plant material in order to perform phytochemical investigations and in the development of technology for the extraction of important metabolites [ 23 ].…”