Xanthotoxin modulates oxidative stress, inflammation, and MAPK signaling in a rotenone-induced Parkinson's disease model

Sayed, Aya Shoukry; Sayed, Nesrine S El; Budzyńska, Barbara; Skalicka‐Woźniak, Krystyna; Ahmed, Mariam K; Kandil, Esraa A.

doi:10.1016/j.lfs.2022.121129

Cited by 12 publications

(6 citation statements)

References 66 publications

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“…Rotenone can induce dopaminergic neuron loss, dopamine level reduction, and alpha-synuclein accumulation, resulting in behavioral defects [ 35 ]. This study obtained rotenone from Shanghai Jingdu Biotechnology Co., Ltd, China.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, training and test were both performed once on the second day. The latency to fall from the rotarod (the performance time) was measured for 5 min and recorded in seconds [ 35 ]. The test was performed with rotarod instrumentation and analyzed by software (Rota Rod Rotamex 5, Columbus Instruments).…”

Section: Methodsmentioning

confidence: 99%

“…In the assessment, each mouse was placed in the middle of the box and allowed to explore freely. To eliminate any possible bias caused by scents left by previous mice, the floor and walls were rinsed with 70% ethyl alcohol after each test [ 35 ]. The total distance traveled was recorded.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Curcumin protects against rotenone-induced Parkinson's disease in mice by inhibiting microglial NLRP3 inflammasome activation and alleviating mitochondrial dysfunction

Hao

et al. 2023

Heliyon

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Curcumin protects against rotenone-induced Parkinson's disease in mice by inhibiting microglial NLRP3 inflammasome activation and alleviating mitochondrial dysfunction

Hao

et al. 2023

Heliyon

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“…Mounting evidence suggests critical involvement of SN neurons in mediating motor functions with optimal production, release and transmission of DA [ 40 ]. However, exposure to environmental toxin may alter the DA circuitry through imparting neurodegeneration and it results in the motor functional deficits [ 41 ]. Medicinal plants contain a variety of biologically active ingredients, as previous studies report hexadecanoic acid (Palmitic acid), choloro benzene, catechol and flavonoids from MP extracts and in vivo studies reveal their neuroprotective, antioxidant, anti-inflammatory and anti-cancerous properties.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective potential of Mentha piperita extract prevents motor dysfunctions in mouse model of Parkinson’s disease through anti-oxidant capacities

Anjum,

Raza,

Faheem

et al. 2024

PLoS ONE

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Parkinson’s disease (PD) is the second most common neurodegenerative disease in the world. Neurodegeneration of the substantia nigra (SN) and diminished release of dopamine are prominent causes of this progressive disease. The current study aims to evaluate the protective potential of ethanolic extract of Mentha piperita (EthMP) against rotenone-mediated PD features, dopaminergic neuronal degeneration, oxidative stress and neuronal survival in a mouse model. Swiss albino male mice were assigned to five groups: control (2.5% DMSO vehicle), PD (rotenone 2.5 mg/kg), EthMP and rotenone (200mg/kg and 2.5mg/kg, respectively), EthMP (200 mg/kg), and Sinemet, reference treatment containing levodopa and carbidopa (20 mg/kg and rotenone 2.5mg/kg). Behavioral tests for motor functional deficit analysis were performed. Anti-oxidant capacity was estimated using standard antioxidant markers. Histopathology of the mid-brain for neurodegeneration estimation was performed. HPLC based dopamine level analysis and modulation of gene expression using quantitative real-time polymerase chain reaction was performed for the selected genes. EthMP administration significantly prevented the rotenone-mediated motor dysfunctions compared to PD group as assessed through open field, beam walk, pole climb down, stepping, tail suspension, and stride length tests. EthMP administration modulated the lipid peroxidation (LPO), reduced glutathione (GSH), and superoxide dismutase (SOD) levels, as well as glutathione-s-transferase (GST) and catalase (CAT) activities in mouse brain. EthMP extract prevented neurodegeneration in the SN of mice and partially maintained dopamine levels. The expression of genes related to dopamine, anti-oxidant potential and synapses were modulated in M. piperita (MP) extract treated mice brains. Current data suggest therapeutic capacities of MP extract and neuroprotective capacities, possibly through antioxidant capacities. Therefore, it may have potential clinical applications for PD management.

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“…Various extrinsic and intrinsic stressors can activate these cascades, leading to a series of phosphorylation events that regulate cellular activities 52 . Emerging evidence suggests that the regulation of the MAPK signaling pathway is critical in PD 53 . The dysregulation of MAPK pathways, particularly JNK and p38, has been associated with neurodegeneration and disease progression in response to neurotoxic insults 54 .…”

Section: Discussionmentioning

confidence: 99%

Carpesii fructus extract exhibits neuroprotective effects in cellular and Caenorhabditis elegans models of Parkinson's disease

Zhu,

Wang,

Qin

et al. 2023

CNS Neurosci Ther

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ObjectiveParkinson's disease (PD) is a debilitating neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the substantia nigra. Despite extensive research, no definitive cure or effective disease‐modifying treatment for PD exists to date. Therefore, the identification of novel therapeutic agents with neuroprotective properties is of utmost importance. Here, we aimed to investigate the potential neuroprotective effects of Carpesii fructus extract (CFE) in both cellular and Caenorhabditis elegans (C. elegans) models of PD.MethodsThe neuroprotective effect of CFE in H2O2‐ or 6‐OHDA‐induced PC‐12 cells and α‐synuclein‐overexpressing PC‐12 cells were investigated by determining the cell viability, mitochondrial damage, reactive oxygen species (ROS) production, apoptosis, and α‐synuclein expression. In NL5901, BZ555, and N2 worms, the expression of α‐synuclein, motive ability, the viability of dopaminergic neurons, lifespan, and aging‐related phenotypes were investigated. The signaling pathway was detected by Western blotting and validated by employing small inhibitors and RNAi bacteria.ResultsIn cellular models of PD, CFE significantly attenuated H2O2‐ or 6‐OHDA‐induced toxicity, as evidenced by increased cell viability and reduced apoptosis rate. In addition, CFE treatment suppressed ROS generation and restored mitochondrial membrane potential, highlighting its potential as a mitochondrial protective agent. Furthermore, CFE reduced the expression of α‐synuclein in wide type (WT)‐, A53T‐, A30P‐, or E46K‐α‐synuclein‐overexpressing PC‐12 cells. Our further findings reveal that CFE administration reduced α‐synuclein expression and improved its induced locomotor deficits in NL5901 worms, protected dopaminergic neurons against 6‐OHDA‐induced degeneration in BZ555 worms, extended lifespan, delayed aging‐related phenotypes, and enhanced the ability of stress resistance in N2 worms. Mechanistic studies suggest that the neuroprotective effects of CFE may involve the modulation of the MAPK signaling pathway, including ERK, JNK, and p38, whereas the interference of these pathways attenuated the neuroprotective effect of CFE in vitro and in vivo.ConclusionOverall, our study highlights the potential therapeutic value of CFE as a neuroprotective agent in the context of PD. Furthermore, elucidation of the active compounds of CFE will provide valuable insights for the development of novel therapeutic strategies for PD.

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Xanthotoxin modulates oxidative stress, inflammation, and MAPK signaling in a rotenone-induced Parkinson's disease model

Cited by 12 publications

References 66 publications

Curcumin protects against rotenone-induced Parkinson's disease in mice by inhibiting microglial NLRP3 inflammasome activation and alleviating mitochondrial dysfunction

Curcumin protects against rotenone-induced Parkinson's disease in mice by inhibiting microglial NLRP3 inflammasome activation and alleviating mitochondrial dysfunction

Neuroprotective potential of Mentha piperita extract prevents motor dysfunctions in mouse model of Parkinson’s disease through anti-oxidant capacities

Carpesii fructus extract exhibits neuroprotective effects in cellular and Caenorhabditis elegans models of Parkinson's disease

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