Xenon Does Not Affect Human Platelet Function In Vitro

Abstract

We sought to determine whether xenon affects platelet glycoprotein expression and platelet-related hemostasis in vitro at a clinically relevant concentration. Human whole blood was stimulated with either adenosine diphosphate or the thrombin receptor agonist peptide (TRAP)-6 after incubation with 65% xenon. Halothane at 2 minimum alveolar anesthetic concentration was used as a positive control. Platelet function and activation were evaluated with two-color flow cytometry. The expression of the platelet glycoproteins GPIIb/IIIa, GPIb, and P selectin were detected with fluorochrome-conjugated monoclonal antibodies. In vitro measurement of platelet-related hemostasis under conditions of high shear stress was performed in citrated whole blood with a platelet function analyzer (PFA-100((R))) by using collagen/epinephrine and collagen/adenosine diphosphate cartridges. Xenon did not affect basal or agonist-induced expression of platelet membrane glycoproteins, activation-dependent conformational changes of the GPIIb/IIIa receptor, expression of P selectin, or PFA closure times. In contrast, halothane reduced TRAP-6-induced activation of the GPIIb/IIIa complex. Furthermore, collagen/epinephrine-induced PFA closure time was significantly prolonged. These results demonstrate that xenon does not affect the unstimulated or agonist-induced platelet glycoprotein expression, activation of GPIIb/IIIa, or platelet-related hemostasis.

Combined Blockade of ADP Receptors and PI3-Kinase p110β Fully Prevents Platelet and Leukocyte Activation during Hypothermic Extracorporeal Circulation

“…Regarding the expression of the GPIbα receptor on platelets as well as vWF binding to platelets in our experiments, no effect of either hypothermic ECC or P 2 Y and PI3K p110β inhibition was observed (Figure 2C and D). It has previously been described that the platelet agonist ADP decreases GPIbα surface expression on platelets [20], [23], [24]. This finding was confirmed in our experiments and indicated intact platelet reactivity before and after hypothermic ECC in the PBS group (Figure 2C).…”

Xenon: from stranger to guardian

“…In addition to a lack of neurotoxic profile [30], prolonged xenon exposure in the critical care setting was not associated with any deterioration in haematological and biochemical variables [3]. These data are consistent with previous reports suggesting that xenon exerts no effect on coagulation [51] or platelet function in vitro [52], nor any effect on the immune system [53]. Xenon does not impair hepatic or renal function [3,54].…”

Xenônio: farmacologia e uso clínico

“…Cortisol concentrations remain unchanged as opposed to nitrous oxide 36 . Platelet function is not changed by xenon 37 .…”

“…As concentrações de cortisol permanecem inalteradas, ao contrário do que ocorre com o óxido nitroso 36 . A função plaquetária não é alterada pelo xenônio 37 .…”