XH1 – a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression

Han, Xin; Lyle, Robert; Eustace, D.; Jewers, Richard J.; Parrington, Jennifer M.; Das, Asha; Chana, T; Dagg, Belinda; Money, Sarah; Bates, Thelma D.

doi:10.1038/bjc.1991.376

Cited by 13 publications

(1 citation statement)

References 50 publications

(46 reference statements)

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“…HPV-16 E-mRNA was detected in K1/16 cells, which are known to express early-region transcripts (28) as well as the E5 protein (22). In addition, E-mRNA was detected in CaSki, XH1, and SiHa cells, all of which contain integrated HPV-16 DNA (2,19,42). In CaSki and SiHa cells, most transcripts use a cellular polyadenylation signal flanking the integrated HPV DNA, but a minority of full-length transcripts in CaSki cells use the defined HPV-16 early polyadenylation signal (2,37,38).…”

Section: Discussionmentioning

confidence: 99%

Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology

Biswas¹,

Kell²,

Mant³

et al. 1997

J Clin Microbiol

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Human papillomavirus type 16 (HPV-16) is associated with abnormal Papanicolou smears, indicative of cervical intraepithelial neoplasia. HPV-16 is the most common genital HPV and is found in up to 40% of young women with normal cervical cytology. In order to investigate whether transcriptionally active HPV-16 infection is associated with abnormal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading frame was developed to detect all HPV-16 early-region mRNA (E-mRNA) transcripts. It was used to study HPV-16-infected women with normal and abnormal cervical cytologies to obtain evidence of active infection. Among HPV-16 DNA-positive women, HPV-16 E-mRNA was detected in 15 of 37 (40.5%) women with abnormal cervical cytology but in only 4 of 35 (11.4%) women with normal cytology (P ‫؍‬ 0.007). Thus, HPV-16 E-mRNA transcription is associated with abnormal cervical smears and may have value as a prognostic marker of progressive disease.

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Section: Discussionmentioning

confidence: 99%

Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology

Biswas¹,

Kell²,

Mant³

et al. 1997

J Clin Microbiol

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The human squamous cervical carcinoma cell line, HOG‐1, is responsive to steroid hormones

White

Jones

Croxtall

et al. 1992

Intl Journal of Cancer

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Growth of the human squamous cervical carcinoma cell line, HOG-I, was stimulated in response to oestradiol in serum-containing and chemically defined medium. The oestradiol-stimulated growth could be inhibited by 4-OH tamoxifen, progesterone and medroxyprogesterone acetate; the last 2 compounds also inhibited basal cell growth in serum-containing and chemically defined media. The data are consistent with the sensitivity of human squamous cervical cancer to sex-steroid hormones and suggest that endocrine therapies may be of benefit in this disease.

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Viral load as a determinant for transmission of human papillomavirus type 16 from mother to child

Kaye¹,

Cason²,

Pakarian³

et al. 1994

Journal of Medical Virology

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Whilst genital papillomaviruses are commonly believed to be sexually transmitted, transmission of human papillomavirus type 16 (HPV-16) from mother to child at delivery has been described previously [Pakarian et al. (in press) British Journal of Obstetrics and Gynaecology]. In order to determine whether viral load in cervical/vaginal cells was an important determinant of transmission 15 pregnant women with HPV-16 infections were studied. Eight of these women had infants who were positive for HPV-16 DNA at genital and/or buccal sites. Viral load was estimated by laser densitometry of polymerase chain reaction (PCR) products. The eight mothers--four with a previous history of abnormal smears and two with previous genital warts--who transmitted infection to their infants had significantly higher viral loads (P < 0.05) than those who did not. It is concluded that viral load is an important, but not the sole, determinant for the transmission of HPV-16 from mother to infant.

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XH1 – a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression

Cited by 13 publications

References 50 publications

Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology

Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology

The human squamous cervical carcinoma cell line, HOG‐1, is responsive to steroid hormones

Viral load as a determinant for transmission of human papillomavirus type 16 from mother to child

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