Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis

Liu, Ming‐Wei; Wang, Yunhui; Qian, Chuanyun; Li, Hui

doi:10.3892/ijmm.2014.1943

Cited by 41 publications

(44 citation statements)

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“…Endotoxin causes different types of tissue and organ damages, and XBJ injection exhibited antagonistic effects against endotoxin toxicity in different animal models [1819]. Of particular interest to our current study, XBJ inhibited the expression of TREM-1 by antagonizing endotoxin [38], through which it decreased the level of Toll-like receptor 4, hence reducing the production of inflammatory cytokines to protect organ functions [39]. …”

Section: Discussionmentioning

confidence: 99%

The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

Liu

Wang

et al. 2016

Korean J Physiol Pharmacol

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Paeoniflorin (PAE) is the most abundant compound in Xuebijing injection widely used to treat sepsis. We aimed to investigate effect of PAE on expression of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in a rat model of sepsis. Wistar rats were divided into Normal, Model, and PAE groups (n=20 each). Endotoxin was administrated at 5 mg/ml/kg in Model and PAE rats to establish rat sepsis model. 1 h after endotoxin administration, PAE was administrated at 4 ml/kg in PAE group once per day for 3 days. Routine blood tests and biochemical indexes were assessed, including aspartate aminotransferase (AST) and creatine kinase-MB (CK-MB). The plasma sTREM-1 level was measured using quantitative ELISA. At the end of experiment, the small intestine, liver, kidney and lung were subjected to pathological examinations. A rat model of sepsis-induced multiple organ dysfunction syndrome (MODS) was established successfully with endotoxin administration (5 mg/ml/kg), evidenced by histo-pathological examinations, routine blood tests and biochemical indexes: platelet count decreased and white blood cell count increased (p<0.05), CK-MB and AST increased (p<0.05). PAE treatment significantly reduced the plasma levels of AST, CK-MB, and sTREM-1, compared to Model group (p<0.05). Meanwhile, sepsis-induced damages in the liver, lung, stomach and intestinal mucosa were also markedly ameliorated by PAE treatment. PAE demonstrated a significantly protective effect in a rat model of sepsis by decreasing plasma sTREM-1 level, reducing inflammation, preventing MODS and protecting organ functions.

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Section: Discussionmentioning

confidence: 99%

The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

Liu

Wang

et al. 2016

Korean J Physiol Pharmacol

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“…As a feature of sepsis, capillary leakage represents an endothelial barrier dysfunction, which can be induced by the secretion of inflammatory cytokines that increase endothelial permeability (26). The dangers of capillary leakage include increased tissue edema, cell hypoxia and the excessive local accumulation of inflammatory cytokines, which directly lead to tissue and cell damage and can induce MODS or MOF (27).…”

Section: Discussionmentioning

confidence: 99%

Expression of HMGB1 in septic serum induces vascular endothelial hyperpermeability

Zheng

Ding

et al. 2015

Molecular Medicine Reports

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The aim of the present study was to investigate the effects of high mobility group protein B1 (HMGB1), which is expressed in the serum of patients with sepsis, on vascular endothelial permeability. Sera from patients with sepsis were used to treat endothelial cells (ECs), and the effect on endothelial permeability was evaluated using immunofluorescence. The morphologies of endothelial cytoskeletal actin and vascular endothelial (VE)‑cadherin were assessed using laser scanning confocal microscopy. The protein expression levels of HMGB1, B‑cell lymphoma 2 (BCL‑2) and BCL‑2‑associated X protein (BAX) were detected using western blotting. EC apoptosis was measured using flow cytometry. The results demonstrated that HMGB1 was significantly expressed in the serum 24 h following the onset of sepsis, and the expression levels peaked at 48 h, which were sustained until 96 h post‑onset. Compared with the control group, treatment of the ECs with 20% septic serum in vitro significantly increased endothelial monolayer permeability (P<0.01), markedly induced transcellular filamentous (F)‑actin rearrangement with stress fiber formation, and resulted in the localization of VE‑cadherin fragmentations at the cell borders with increased gaps between ECs. Furthermore, flow cytometry showed that the apoptotic rate of ECs was significantly increased following treatment with septic serum. In addition, the expression levels of BAX were significantly increased, whereas the expression levels of BCL‑2 were significantly decreased. Pretreatment with an HMGBI inhibitor (ethyl pyruvate; 5 µM) 24 h prior to treatment with the septic serum attenuated the effects of septic serum treatment. Together, these findings suggested that treatment of ECs with sera from patients with sepsis may induce the loss of vascular endothelial monolayer integrity, elicit the formation of endothelial F‑actin stress fibers and initiate VE‑cadherin redistribution, which may be attributed to high levels of HMGB1 in the serum. This mechanism also appears to involve changes in the activation of BAX and BCL‑2, resulting in EC apoptosis.

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“…[89] Xuebijing, a traditional Chinese medicine preparation, has functions of anti-infl ammation, neutralizing endotoxin and regulating immune cells, etc. Animal experiments [90,91] showed that Xuebijing reduced the mortality of septic mice. A single center clinical study found that Xuebijing could promote the stabilization of hemodynamics and improve the outcome of patients with sepsis complicated with DIC.…”

Section: Subcutaneous Injection Of Thymosin and Intravenousmentioning

confidence: 99%

Expert consensus on the perioperative management of patients with sepsis

Anesthesiologists¹

2015

World Journal of Emergency Medicine

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Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis

Cited by 41 publications

References 50 publications

The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

Expression of HMGB1 in septic serum induces vascular endothelial hyperpermeability

Expert consensus on the perioperative management of patients with sepsis

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