Xylan catabolism is improved by blending bioprospecting and metabolic pathway engineering in <i>Saccharomyces cerevisiae</i>

Lee, Sun Mi; Jellison, Taylor; Alper, Hal S.

doi:10.1002/biot.201400622

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…In a more recent study, a blended bioprospecting approach was applied ( Lee et al, 2015 ) along with rational and evolutionary engineering to improve xylan assimilation in an engineered xylan-catabolizing S. cerevisiae strain. The extracellular expression of xylan active enzymes (xylanase 1—XNA1, xylosidase 2—XD2, and arabinofuranosidase—AFB) from Ustilago bevomyces were cloned into 2-µm plasmids, p423 and p424 under the control of the GPD promoter.…”

Section: Ethanol Production From Xos By Extracellular Expression Of X...mentioning

confidence: 99%

Xylo-Oligosaccharide Utilization by Engineered Saccharomyces cerevisiae to Produce Ethanol

Procópio

Kendrick

Goldbeck

et al. 2022

Front. Bioeng. Biotechnol.

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The engineering of xylo-oligosaccharide-consuming Saccharomyces cerevisiae strains is a promising approach for more effective utilization of lignocellulosic biomass and the development of economic industrial fermentation processes. Extending the sugar consumption range without catabolite repression by including the metabolism of oligomers instead of only monomers would significantly improve second-generation ethanol production This review focuses on different aspects of the action mechanisms of xylan-degrading enzymes from bacteria and fungi, and their insertion in S. cerevisiae strains to obtain microbial cell factories able of consume these complex sugars and convert them to ethanol. Emphasis is given to different strategies for ethanol production from both extracellular and intracellular xylo-oligosaccharide utilization by S. cerevisiae strains. The suitability of S. cerevisiae for ethanol production combined with its genetic tractability indicates that it can play an important role in xylan bioconversion through the heterologous expression of xylanases from other microorganisms.

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Section: Ethanol Production From Xos By Extracellular Expression Of X...mentioning

confidence: 99%

Xylo-Oligosaccharide Utilization by Engineered Saccharomyces cerevisiae to Produce Ethanol

Procópio

Kendrick

Goldbeck

et al. 2022

Front. Bioeng. Biotechnol.

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“…Oxidoreductase xylose consumption pathway. XylA, Xyn, ABF ( Ustilago bevomyces ) 20 g/L of xylan 0.32 N.D. [ 134 ] Hemicellulosome. Scaffoldin C. thermocellum XylA ( A. niger ); Xyn ( T. reesei ) 10 g/L birchwood xylan 0.95 N.D. [ 149 ] Cell-surface display.…”

Section: Consolidated Bioprocessingmentioning

confidence: 99%

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

et al. 2020

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The biorefinery concept, consisting in using renewable biomass with economical and energy goals, appeared in response to the ongoing exhaustion of fossil reserves. Bioethanol is the most prominent biofuel and has been considered one of the top chemicals to be obtained from biomass. Saccharomyces cerevisiae, the preferred microorganism for ethanol production, has been the target of extensive genetic modifications to improve the production of this alcohol from renewable biomasses. Additionally, S. cerevisiae strains from harsh industrial environments have been exploited due to their robust traits and improved fermentative capacity. Nevertheless, there is still not an optimized strain capable of turning second generation bioprocesses economically viable. Considering this, and aiming to facilitate and guide the future development of effective S. cerevisiae strains, this work reviews genetic engineering strategies envisioning improvements in 2 nd generation bioethanol production, with special focus in process-related traits, xylose consumption, and consolidated bioprocessing. Altogether, the genetic toolbox described proves S. cerevisiae to be a key microorganism for the establishment of a bioeconomy, not only for the production of lignocellulosic bioethanol, but also having potential as a cell factory platform for overall valorization of renewable biomasses.

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“…Thus, the direct conversion of hemicellulose into ethanol requires the simultaneous expression of xylan-degrading enzymes and xylose-assimilating enzymes in the recombinant S. cerevisiae strain. Despite promising advantages of CBP process over other alternatives, few attempts have been reported for the direct conversion of hemicellulose into ethanol, achieving ethanol concentrations in the range from 0.32 to 8.2 g/L [6,[8][9][10][11][12]. The most remarkable results with cell-surface display in a hemicellulosic CBP were obtained with liquors derived from hydrothermal treatment of rice straw, in which 4.04 and 8.2 g/L of ethanol were produced by engineered industrial S. cerevisiae Sun049 strain and engineered laboratorial S. cerevisiae NBRC1440/X strain, respectively [6,8].…”

mentioning

confidence: 99%

Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

Cunha

Romaní

Inokuma

et al. 2020

Biotechnol Biofuels

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Background: Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which contains xylose as a major component in concentrations that can reach up to 40% of the total biomass in hardwoods and agricultural residues. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae-the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of corn cobderived hemicellulose. Results: These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of corn cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 g/g of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated corn cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. Conclusions: These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.

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Xylan catabolism is improved by blending bioprospecting and metabolic pathway engineering in Saccharomyces cerevisiae

Cited by 9 publications

References 39 publications

Xylo-Oligosaccharide Utilization by Engineered Saccharomyces cerevisiae to Produce Ethanol

Xylo-Oligosaccharide Utilization by Engineered Saccharomyces cerevisiae to Produce Ethanol

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

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