Xylan-cellulose thin film platform for assessing xylanase activity

Schaubeder, Jana B.; Ravn, Jonas Laukkonen; Orzan, Eliott Jean Quentin; Manfrão-Netto, João H.C.; Geijer, Cecilia; Nypelö, Tiina; Spirk, Stefan

doi:10.1016/j.carbpol.2022.119737

Cited by 12 publications

(5 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cellulose films (Figure a–c) show a fibrillar surface structure with a preferred orientation. At lower resolutions and when measured in a dry state, these thin films appear to have spherical features, ,− but at a higher resolution, the fibrillar structures of the cellulose thin films become apparent, which are more pronounced in liquid-state measurements. The diameter of these fibrils can be determined by measuring the height profile of at least three tightly packed, parallel fibrils using the width of the central fibril according to Figure S1.…”

Section: Resultsmentioning

confidence: 99%

“…For the MP-SPR Navi 210A VASA used in this study, the k·dp value is about 1.90 × 10 –7 cm·° –1 for the 785 nm laser in aqueous systems. The refractive index increment (d n /d c ) of 0.158 cm 3 ·g –1 was used as reported for xylan normalΓ = normalΔ normalΘ · k · d p normald n / normald c …”

Section: Methodsmentioning

confidence: 99%

“…Silicon wafers were cut into 1 × 1 cm 2 pieces and cleaned prior to use by immersion in the acid piranha solution containing H 2 O 2 (30 wt %)/H 2 SO 4 (1:2 v/v) for 16 min, followed by rinsing with distilled water for several minutes and subsequently dried in a nitrogen stream. Xylan was dissolved in DMSO at different concentrations of 10 and 20 mg·mL –1 aided by activation with deionized water according to Schaubeder et al 19 Lignin was dissolved directly in DMSO at a concentration of 20 mg·mL –1 . For the production of the blend films, lignin powder was added in the corresponding amounts to the predissolved xylan solution.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

How Resilient is Wood Xylan to Enzymatic Degradation in a Matrix with Kraft Lignin?

Schaubeder,

Ganser,

Nypelö

et al. 2024

Biomacromolecules

Self Cite

View full text Add to dashboard Cite

Despite the potential of lignocellulose in manufacturing value-added chemicals and biofuels, its efficient biotechnological conversion by enzymatic hydrolysis still poses major challenges. The complex interplay between xylan, cellulose, and lignin in fibrous materials makes it difficult to assess underlying physico-and biochemical mechanisms. Here, we reduce the complexity of the system by creating matrices of cellulose, xylan, and lignin, which consists of a cellulose base layer and xylan/lignin domains. We follow enzymatic degradation using an endoxylanase by high-speed atomic force microscopy and surface plasmon resonance spectroscopy to obtain morphological and kinetic data. Fastest reaction kinetics were observed at low lignin contents, which were related to the different swelling capacities of xylan. We demonstrate that the complex processes taking place at the interfaces of lignin and xylan in the presence of enzymes can be monitored in real time, providing a future platform for observing phenomena relevant to fiber-based systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

How Resilient is Wood Xylan to Enzymatic Degradation in a Matrix with Kraft Lignin?

Schaubeder,

Ganser,

Nypelö

et al. 2024

Biomacromolecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…This means that the structure and chemistry of the adsorbate along with the surface morphology and adsorption conditions (e.g., adsorbate concentration, pH value, and salt concentration) govern the overall adsorption. Surface-sensitive techniques with nanoscale resolution such as quartz crystal microbalance with dissipation monitoring and surface plasmon resonance are typically employed for investigating solid–liquid adsorption, and both techniques have been successfully implemented in solid–liquid adsorption studies involving hemicelluloses. − …”

Section: Key Physicochemical Properties Of Wood Hemicelluloses and Th...mentioning

confidence: 99%

Potential of Wood Hemicelluloses and Their Derivates as Food Ingredients

Abik

Palasingh

Bhattarai

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

A holistic utilization of all lignocellulosic wood biomass, instead of the current approach of using only the cellulose fraction, is crucial for the efficient, ecological, and economical use of the forest resources. Use of wood constituents in the food and feed sector is a potential way of promoting the global economy. However, industrially established food products utilizing such components are still scarce, with the exception of cellulose derivatives. Hemicelluloses that include xylans and mannans are major constituents of wood. The wood hemicelluloses are structurally similar to hemicelluloses from crops, which are included in our diet, for example, as a part of dietary fibers. Hence, structurally similar wood hemicelluloses have the potential for similar uses. We review the current status and future potential of wood hemicelluloses as food ingredients. We include an inventory of the extraction routes of wood hemicelluloses, their physicochemical properties, and some of their gastrointestinal characteristics, and we also consider the regulatory route that research findings need to follow to be approved for food solutions, as well as the current status of the wood hemicellulose applications on that route.

show abstract

“…[20,30] Besides, biomass CDs usually has many groups on its surface, good water solubility and easy functionalization, which provides in nite possibilities for further modi cation of materials. [25,31] Therefore, with the in-depth study of biomass CDs, it will broaden the eld of uorescence sensing.…”

Section: Introductionmentioning

confidence: 99%

Xylan derived carbon dots composite with cotton cellulose paper as fluorescence sensor for real time detection Cu2+

Zhang

Feng

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

Cotton cellulose paper composited with fluorescence carbon dots (CDs) has shown potential for Cu2+ detection in environmental monitoring. In this study, a solid-state detection platform was developed using biomass-based fluorescent sensors (CPU-CDs) composed of xylan-derived CDs (U-CDs) and cotton cellulose paper. The fluorescence platform was nanoengineered to monitor Cu2+ changes via spectral and colorimetric dual-modal methods. CPU-CDs exhibits reusability, non-toxicity, excellent fluorescence characteristics and biocompatibility. Besides, CPU-CDs has a complex network structure and a large number of hydroxyl and amino groups, which can realize a high loading rate of U-CDs and provide more binding sites for the detection and response of Cu2+. CPU-CDs displays high effectiveness and sensitivity for Cu2+. Additionally, the detection limit of CPU-CDs for Cu2+ as low as 0.14 μM that was well below U.S. EPA safety levels (20 μM). Practical application indicated that CPU-CDs could achieve precision response of Cu2+ change in water environment with recovery range of 90%-119%. This strategy demonstrated a promising biomass solid-state fluorescence sensor for Cu2+ detection for water treatment research.

show abstract

Xylan-cellulose thin film platform for assessing xylanase activity

Cited by 12 publications

References 71 publications

How Resilient is Wood Xylan to Enzymatic Degradation in a Matrix with Kraft Lignin?

How Resilient is Wood Xylan to Enzymatic Degradation in a Matrix with Kraft Lignin?

Potential of Wood Hemicelluloses and Their Derivates as Food Ingredients

Xylan derived carbon dots composite with cotton cellulose paper as fluorescence sensor for real time detection Cu2+

Contact Info

Product

Resources

About