Xylanase, Glucanase, and Phytase in the Diet of Light Laying Hens

Lima, Matheus Ramalho de; Costa, Fernando Guilherme Perazzo; Vieira, Danilo Vargas Gonçalves; Cardoso, Anilma Sampaio; Lima, G. S.; Cavalcante, Danilo Teixeira; Pinheiro, Sarah Gomes; Ceccantini, Márcio; Yavuz, Baris; Bezerra, Roseane Madeira; Kaneko, Isabelle Naemi

doi:10.3382/japr/pfz081

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…Interestingly, the change in yolk color was slight but significant. Several researchers have previously reported the absence of xylanase effects on yolk color [ 32 – 34 ]. The improvement in yolk color obtained in the last experimental phase can be explained by the increase in apparent crude fat digestibility in these treatments.…”

Section: Discussionmentioning

confidence: 99%

Xylanase impact beyond performance: A microbiome approach in laying hens

et al. 2021

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Anti-nutritional compounds such as non-starch polysaccharides (NSP) are present in viscous cereals used in feed for poultry. Therefore, exogenous carbohydrases are commonly added to monogastric feed to degrade these NSP. Our hypothesis is that xylanase not only improves laying hen performance and digestibility, but also induces a significant shift in microbial composition within the intestinal tract and thereby might exert a prebiotic effect. In this context, a better understanding on whether and how the chicken gut microbial population can be modulated by xylanase is required. To do so, the effects of dietary supplementation of xylanase on performance, apparent total tract digestibility (ATTD) and cecal microbiome in laying hens were evaluated in the present study. A total of 96 HiSex laying hens were used in this experiment (3 diets and 16 replicates of 2 hens). Xylanase was added to the diets at concentrations of 0, 45,000 (15 g/t XygestTM HT) and 90,000 U/kg (30 g/t Xygest HT). The diets were based on wheat (~55%), soybean and sunflower meal. The lowest dosage, 45,000 U/kg, significantly increased average egg weight and improved feed efficiency compared to the control treatment (P<0.05). Egg quality parameters were significantly improved in the experiment in response to the xylanase addition. For example, during the last 28 days of the trial, birds receiving the 45,000 U/kg and the 90,000 U/kg treatments exhibited an increase in Haugh units and albumin heights (P<0.05). Compared with the control, the ATTD of organic matter and crude protein were drastically improved in the 45,000 U/kg treatment group (P<0.05). Furthermore, gross energy and the ATTD of crude fat were improved significantly for birds fed 90,000 U/kg group compared to the control. Importantly, 16S rRNA gene analysis revealed that xylanase at 45,000 U/kg dosage can exert a change in the cecal microbiome. A significant increase in beneficial bacteria (Bacilli class; Enterococcaceae and Lactobacillales orders; Merdibacter, Enterococcus and Nocardiopsis genera; Enterococcus casseliflavus species) was documented when adding 45,000 U/kg xylanase to the diet of laying hens. In conclusion, dietary supplementation of xylanase 45,000 U/kg significantly improved laying hen performance and digestibility. Furthermore, microbiome data suggest that xylanase modulates the laying hen bacterial population beneficially, thus potentially exerting a prebiotic effect.

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Section: Discussionmentioning

confidence: 99%

Xylanase impact beyond performance: A microbiome approach in laying hens

et al. 2021

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“…Soluble fibers are indigestible by nonruminants at the intestinal level, which could explain the reduction in food energy use, and consequently, impair the absorption of minerals and affect the metabolism of lipids. However, the efficient digestion of soluble fibers may be directly reflected in the results of other metabolizability coefficients (Lima et al, 2019).…”

Section: Test1mentioning

confidence: 99%

“…The increased viscosity of the intestinal digest caused by the amount of NSPs is considered the most important mechanism for the low utilization of wheat bran (Yaghobfar & Kalantar, 2017). Wheat-based diets have more than twice the concentration of NSPs than corn-based diets and soybean meal, implying a higher viscosity of the digestive tract in poultry (Lima et al, 2019). Evaluating the effects of xylanase, glucanase, and phytase in the diet of light laying hens, Lima et al (2019), observed that enzymatic supplementation is efficient for light laying, similar to the present study.…”

Section: Testmentioning

confidence: 99%

“…Additionally, the lack of endogenous enzymes could compromise the energy content of foods by maintaining energygenerating nutrients, such as carbohydrates, lipids, and proteins, within their encapsulated structures by the anti-nutritional factor (Lima et al, 2019). Diets with enzyme complex supplementation demonstrated that MCDM and AMEn were superior in diets with enzyme supplementation compared to diets without supplementation.…”

Section: Testmentioning

confidence: 99%

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Xylanases in diets with alternative feeds and energy reduction in commercial layers

Andrade

Garcia

Kiefer

et al. 2021

Semina: Ciênc. Agrár.

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The use of alternative ingredients has been increasing in a continuing attempt to reduce production costs, along with the use of additives, such as carbohydrases, for their possible positive effects on nutrient metabolization by layers. Thus, this study aimed to evaluate the effects of different xylanases in diets with reduced metabolizable energy (ME) and the inclusion of alternative ingredients on the metabolizability of nutrients in diets for commercial laying hens, by conducting two metabolism tests. In the first trial,100 layers hens were distributed in a fully randomized 2 × 2 + 1 factorial design (two metabolizable energy reductions × two xylanases, plus one control diet). In the second assay,140 layer hens were distributed in a fully randomized 2 × 3 + 2 factor design (two xylanases× three ingredients, plus two control diets, positive and negative). It was concluded that supplementation with pantanal xylanase improved the metabolizability of nutrients in corn-based diets and soybean meal for light commercial layers, allowing for a reduction of up to 200 kcal/kg of ME in the diets. Xylanases are more effective on wheat bran enabling a decrease of 150 kcal/kg of ME in commercial laying diets without affecting nutrient metabolizability. The action of pantanal xylanase in diets containing fibrous ingredients was similar to that of commercial xylanase.

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“…Xylanases, a group of microbially produced hydrolytic enzymes are capable to break down xylan into xylose [ 3 ], resulting in reduction of gut viscosity, release of encapsulated nutrients and increase in dietary energy available [ 4 , 5 ]. So far, studies with dietary supplementation of xylanase in laying hens have shown improved productivity parameters and nutrient utilization [ 2 , 6 , 7 , 8 ]. More specifically, a study conducted by Bobeck et al [ 7 ] showed that the beneficial effects of xylanase supplementation in layers’ diets include increased egg production, egg mass and feed intake.…”

Section: Introductionmentioning

confidence: 99%

Xylanase Supplementation in Wheat-Based Diets of Laying Hens Affects the Egg Yolk Color, Carotenoid and Fatty Acid Profiles

Papadopoulos

Lioliopoulou

Ordoudi

et al. 2022

Foods

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Wheat is rich in non-starch polysaccharides (NSP) and their degradation in poultry diets is promoted by exogenous carbohydrases. The objective here was to evaluate the effect of adding an intrinsically thermostable xylanase on wheat-based diets for laying hens in yolk color, carotenoid and fatty acid profiles of eggs. A total of 128 laying hens were used for 12 weeks. They were randomly allocated to four dietary treatments with different levels of xylanase: T1: control (no xylanase), T2: 30,000 U/g, T3: 45,000 U/g and T4: 90,000 U/g, with 32 birds, 16 replicates per treatment (2 birds/replicate). At the end of the experimental period, egg yolk color index, redness (a*) and yellowness (b*) of egg yolks were found significantly higher in all the enzyme supplemented diet groups (T2, T3, T4) compared with the control (T1). Canthaxanthin levels were significantly higher in T3 than T1 (p < 0.05). Total n-3, n-6 and total polyunsaturated fatty acids (FAs) were significantly higher in T4 compared with the control (p < 0.01), while the reverse trend was evidenced for monounsaturated FAs. Additionally, total n-3 FAs were higher in the T2 than T1 (p < 0.005). Overall, the results showed that exogenous xylanase enzyme supplementation in wheat-based diets for laying hens contribute to maintaining egg yolk color. Overall, exogenous xylanase enzyme supplemented at all levels in wheat-based laying hens’ diets improved egg yolk color compared to the control diet. The enzyme supplemented at the higher level (90,000 U/g) improved polyunsaturated and reduced monounsaturated egg yolk fatty acid content.

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Xylanase, Glucanase, and Phytase in the Diet of Light Laying Hens

Cited by 7 publications

References 14 publications

Xylanase impact beyond performance: A microbiome approach in laying hens

Xylanase impact beyond performance: A microbiome approach in laying hens

Xylanases in diets with alternative feeds and energy reduction in commercial layers

Xylanase Supplementation in Wheat-Based Diets of Laying Hens Affects the Egg Yolk Color, Carotenoid and Fatty Acid Profiles

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