Xylem water cryogenic vacuum extraction: Testing correction methods with CaviTron-based apple twig sampling

Dong, Haiqing; Wen, Mingyi; Li, Han; Du, Guangyuan; Zhang, Congcong; He, Hailong; Jin, Jingjing; Li, Min; Si, Bingcheng

doi:10.1016/j.jhydrol.2023.129572

Cited by 11 publications

(18 citation statements)

References 42 publications

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“…Finally, tests of CVE enrichment have been robust for variations in RWC (Wen et al, 2022), water volumes (Diao et al, 2022), or plant species (Chen et al, 2020), but not for all variables at once. New experiments that co‐vary RWC, water volumes, and species may support new findings (Zhao, 2021) that either refute or support universal water isotopic bias corrections (Chen et al, 2020; He et al, 2023).…”

Section: Introductionmentioning

confidence: 83%

“…Recent studies have advanced towards a goal of developing a CVE isotopic δ 2 H bias correction (Zhao, 2021; He et al, 2023) by using experiments that quantify the degree of isotopic change during plant stem rehydration with a water of known isotopic composition. However, the degree of isotopic alteration during rehydration experiments will likely be affected by the isotopic composition of exchangeable hydrogen within cellulose, the time to hydrogen equilibration, and the relative abundance of exchangeable H and water.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for variations in cryogenic extraction deuterium biases of plant xylem water across foundational northeastern US trees

Sobota,

Li,

Hren

et al. 2024

Hydrological Processes

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Measurements of oxygen and hydrogen isotopes in plant xylem water (2H, 18O) have helped to redefine conceptual and numerical models of the hydrological cycle and understand how plants compete for subsurface water. Recent experiments have shown that Cryogenic Vacuum Extraction (CVE) of plant xylem water can result in a δ2H bias. We tested if CVE δ2H‐biases varied significantly across seven foundational northeastern US forest trees with a series of tree core rehydration experiments. Our analysis demonstrated that CVE δ2H‐biases were well predicted by sample gravimetric water content and varied significantly with tree species identity. We show that species‐level δ2H‐bias corrections can result in substantially different understandings of plant water uptake and transpiration versus uncorrected data or generic bias corrections. This research demonstrates an urgent need for the critical evaluation of CVE for plant water extraction. In the absence of a stronger understanding of CVE δ2H‐biases, we recommend that xylem water δ2H observations should not be used in plant water uptake studies.

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Evidence for variations in cryogenic extraction deuterium biases of plant xylem water across foundational northeastern US trees

Sobota,

Li,

Hren

et al. 2024

Hydrological Processes

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“…The rationale for this design was the more roots and water present in a given soil region, the greater the likelihood of water uptake (Mahindawansha et al, 2018). Furthermore, EWC should be a better indicator of water uptake potential than actual water content, as EWC reflects only water that is available for plants to uptake (He et al, 2023). By contrast, actual water content reflects all water, some of which is bound too tightly for plants to withdraw (Oerter et al, 2019).…”

Section: Discrete Probabilistic Mixing Model-mixsiarmentioning

confidence: 99%

A process‐based water stable isotope mixing model for plant water sourcing

Neil,

Fu,

2024

Ecohydrology

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Stable isotopes of hydrogen and oxygen in water are common tools for investigating water uptake apportionment, but many of the existing methods rely on simple linear mixing approaches that do not mechanistically incorporate additional information about site physical properties and conditions. Here, we develop a ‘physically based root water uptake isotope mixing estimation’ model (PRIME) that combines a continuous and parametric probability density function for root water uptake with site physical data in a process‐based linear mixing framework. To demonstrate the application of PRIME, water uptake patterns of boreal forest Pinus banksiana trees were estimated on four dates in 2019. To aid in validation, estimates were compared with that of the Bayesian linear mixing model framework, MixSIAR. The two approaches provided similar results, but due to its continuous and parametric nature, PRIME provided estimates of superior resolution, certainty, and model parsimony. Although both models incorporate additional physical information into their mixing frameworks, PRIME does so in a mechanistic manner, thereby reflecting the relevant hydrological processes more effectively than the purely empirical approach taken by MixSIAR. Furthermore, because PRIME uses a continuous function to describe the predicted uptake pattern, it allows users to quantify water uptake with essentially infinite resolution, through integration over the desired depth ranges. These findings demonstrate the advantages of utilizing a continuous, parametric, and process‐based mixing model to estimate root water uptake apportionment, thus providing a relatively simple yet powerful tool with which to approach plant water sourcing.

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“…Conversely, field studies often lack source water isotopic data, making bias inferences uncertain (Bowers & Williams, 2022; Zuecco et al, 2022). To our knowledge, Barbeta et al (2022) and He et al (2023) are the only authors who have investigated the differences between xylem water, bulk stem water and source water under natural field conditions. However, both studies focused on a limited number of tree species, warranting further investigation into how CVD‐induced biases may vary across different species.…”

Section: Introductionmentioning

confidence: 99%

“…As an alternative to CVD approaches, the Cavitron, a custom‐made rotor fitted to a centrifuge (Cochard, 2002; Cochard et al, 2005), is now emerging as a powerful tool for non‐destructive extraction of xylem water and subsequent evaluation of CVD‐induced isotopic biases (Barbeta et al, 2022; He et al, 2023; Wen et al, 2022). In this study, we use Cavitron‐extracted xylem water as a reference to assess the extent and magnitude of CVD‐induced biases across several tree species under natural field conditions in the Australian tropics.…”

Section: Introductionmentioning

confidence: 99%

Cavitron extraction of xylem water suggests cryogenic extraction biases vary across species but are independent of tree water stress

Duvert,

Barbeta,

Hutley

et al. 2024

Hydrological Processes

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Cryogenic vacuum distillation (CVD) is a widely used technique for extracting plant water from stems for isotopic analysis, but concerns about potential isotopic biases have emerged. Here, we leverage the Cavitron centrifugation technique to extract xylem water and compare its isotopic signature to that of CVD‐extracted bulk stem water as well as source water. Conducted under field conditions in tropical northern Australia, our study spans seven tree species naturally experiencing a range of water stress levels. Our findings reveal a significant deuterium bias in CVD‐extracted bulk stem water when compared to xylem water (median bias −14.9‰), whereas xylem water closely aligned with source water (median offset −1.9‰). We find substantial variations in deuterium bias among the seven tree species (bias ranging from −19.3‰ to −9.1‰), but intriguingly, CVD‐induced biases were unrelated to environmental factors such as relative stem water content and predawn leaf water potential. These results imply that inter‐specific differences may be driven by anatomical traits rather than tree hydraulic functioning. Additionally, our data highlight the potential to use a site‐specific deuterium offset, based on the isotopic signature of local source water, for correcting CVD‐induced biases.

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Xylem water cryogenic vacuum extraction: Testing correction methods with CaviTron-based apple twig sampling

Cited by 11 publications

References 42 publications

Evidence for variations in cryogenic extraction deuterium biases of plant xylem water across foundational northeastern US trees

Evidence for variations in cryogenic extraction deuterium biases of plant xylem water across foundational northeastern US trees

A process‐based water stable isotope mixing model for plant water sourcing

Cavitron extraction of xylem water suggests cryogenic extraction biases vary across species but are independent of tree water stress

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