Y-Box Binding Protein 1 Regulates Angiogenesis in Bladder Cancer via miR-29b-3p-VEGFA Pathway

Abstract

Angiogenesis plays a vital role in the development of bladder cancer (BC). The Y-box-binding protein 1 (YB-1) is a well-known oncoprotein which is closely related to angiogenesis of tumors, but the relationship and mechanism of YB-1 and angiogenesis in BC remain unclear. Based on 56 clinical BC specimens, this study found that high expression of YB-1 samples demonstrated a higher expression of vascular endothelial growth factor A (VEGFA) than those of YB-1 low expression. Subsequently, the expression of YB-1 and miR-29b-3p was regulated in the BC cell lines where we noted that YB-1 promoted VEGFA expression by downregulating the expression of miR- 29b-3p. The ability of BC cells to induce angiogenesis decreased after YB-1 was knocked down. Moreover, the in vivo study further confirmed that YB-1 promotes angiogenesis in BC. Our findings enhance the understanding of how YB-1 promotes angiogenesis in BC and provide evidence for YB-1 as a therapeutic target of BC. Moreover, this may provide new inspiration for miRNAs replacement therapies.

ERK/RSK-mediated phosphorylation of Y-box binding protein-1 aggravates diabetic cardiomyopathy by suppressing its interaction with deubiquitinase OTUB1

“…Besides, it has been shown to play an important role in cardiac pathogenesis by regulating the cardiac apoptosis (11), cardiac fibrosis (12,13), cardiac fetal gene expression, and pathological remodeling of the heart (14). Furthermore, YB-1 has been reported to regulate angiogenesis in various tumors (15,16). Considering the involvement of YB-1 in DCM-related pathogenesis, we speculate that it could be a potential intracellular target in the occurrence and progression of DCM.…”

Gastrointestinal cancers, ACE-2/TMPRSS2 expression and susceptibility to COVID-19

“…The expression of ACE-2 in cancerous cells has been subject to speculations, although ACE-2 upregulation seems to have a protective role against tumour progression being associated with favorable prognosis [ 37 , 38 ]. Whilst AT1 receptor promotes the angiogenesis by inducing VEGF production and uncontrolled cell proliferation of tumoral cells [ 44 ], the interaction of Angiotensin [ 1 – 7 ] with MasR inhibits genesis, abnormal proliferation and progression of tumors [ 45 , 46 ].…”

“…Similar to ACE-2, TMPRSS2 is over-expressed in prostate adenocarcinoma, lung [ 53 ] and colorectal cancer, becoming an established tumour biomarker [ 37 , 39 , 53 ].…”

An Overview of Angiogenesis in Bladder Cancer

“…The panel, including 145 genes involved in angiogenesis, was retrieved from the Uniport database and the published work Single gene expressions of ECM1, FN1, FGF1, FAP, JAM3, THBS1, MFGE8, and COL8A2 involved in angiogenesis and associated with prognosis Dong et al [ 68 ] NRP1 The human bladder immortalised epithelium cell line SVHUC1 and BC cell lines including T24, 5637, J82, UMUC3, and RT4 High expression of NRP1 was observed in BC tissues and cells. NRP1 knockdown promotes apoptosis and suppresses proliferation, angiogenesis, migration, and invasion of BC cells Gao et al [ 69 ] YB-1 Radical cystectomy BC tissue samples Human BC cell lines EJ, UMUC3, SW780, RT4, and the human endothelium cell line EA.hy926 YB-1 promotes angiogenesis in BC. High expression of YB-1 is associated with a higher expression of VEGFA Li et al [ 70 ] CTSB Blood samples and tissue samples from BC patients CTSB is upregulated in tumour tissues and serum extracellular vesicles.…”