YdfH Identified as a Repressor of<i>rspA</i>by the Use of Reduced Genome<i>Escherichia coli</i>MGF-01

Sakihama, Yuri; Mizoguchi, Hiroshi; Oshima, Taku; Ogasawara, Nagahisa

doi:10.1271/bbb.120273

Cited by 5 publications

(1 citation statement)

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“…For example, leukotriene A4 hydrolase is typically involved in alleviating inflammation ( 40 ); thus, downregulation of this gene could lead to the promotion of an inflammatory state typical of periodontal disease. Downregulation of starvation-sensing protein RspA leads to an increase in the production of metabolites that signal starvation ( 41 ) and thus can lead to increased survival during nutrient limitation.…”

Section: Discussionmentioning

confidence: 99%

Microbiota and Metatranscriptome Changes Accompanying the Onset of Gingivitis

Nowicki

Shroff

Singleton

et al. 2018

mBio

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Over half of adults experience gingivitis, a mild yet treatable form of periodontal disease caused by the overgrowth of oral microbes. Left untreated, gingivitis can progress to a more severe and irreversible disease, most commonly chronic periodontitis. While periodontal diseases are associated with a shift in the oral microbiota composition, it remains unclear how this shift impacts microbiota function early in disease progression. Here, we analyzed the transition from health to gingivitis through both 16S v4-v5 rRNA amplicon and metatranscriptome sequencing of subgingival plaque samples from individuals undergoing an experimental gingivitis treatment. Beta-diversity analysis of 16S rRNA reveals that samples cluster based on disease severity and patient but not by oral hygiene status. Significant shifts in the abundance of several genera occurred during disease transition, suggesting a dysbiosis due to development of gingivitis. Comparing taxonomic abundance with transcriptomic activity revealed concordance of bacterial diversity composition between the two quantification assays in samples originating from both healthy and diseased teeth. Metatranscriptome sequencing analysis indicates that during the early stages of transition to gingivitis, a number of virulence-related transcripts were significantly differentially expressed in individual and across pooled patient samples. Upregulated genes include those involved in proteolytic and nucleolytic processes, while expression levels of those involved in surface structure assembly and other general virulence functions leading to colonization or adaptation within the host are more dynamic. These findings help characterize the transition from health to periodontal disease and identify genes associated with early disease.

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Section: Discussionmentioning

confidence: 99%