Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules

Rojas, Marta; Farr, George W.; Fernández, César Fernández; Lauden, Laura; McCormack, John C.; Wolin, Sandra L.

doi:10.1371/journal.pone.0052824

Cited by 30 publications

(29 citation statements)

References 72 publications

(113 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In any case, the fast decrease of c-myc levels is considered a crucial step causing apoptosis in cancer [69], Alzheimer disease [70] and cellular stress [71]. Our results demonstrate that the H 2 O 2 oxidative stress treatment induces a downregulation in CNBP gene expression in hESC and Hs27 fibroblasts.…”

Section: Discussionmentioning

confidence: 68%

Global gene expression profiling and senescence biomarker analysis of hESC exposed to H2O2 induced non-cytotoxic oxidative stress

et al. 2017

View full text Add to dashboard Cite

BackgroundHuman embryonic stem cells (hESCs) potentially offer new routes to study, on the basis of the Developmental Origins of Health and Disease (DOHaD) concept, how the maternal environment during pregnancy influences the offspring’s health and can predispose to chronic disease in later life. Reactive oxygen species (ROS), antioxidant defences and cellular redox status play a key function in gene expression regulation and are involved in diabetes and metabolic syndromes as in ageing.MethodsWe have, therefore, designed an in vitro cell model of oxidative stress by exposing hESCs to hydrogen peroxide (H2O2) during 72 h, in order to resemble the period of preimplantation embryonic development.ResultsWe have analysed the global gene expression profiles of hESCs (HUES3) exposed to non-cytotoxic H2O2 concentrations, using Illumina microarray HT-12 v4, and we found the differential expression of 569 upregulated and 485 downregulated genes. The most affected gene ontology categories were those related with RNA processing and splicing, oxidation reduction and sterol metabolic processes. We compared our findings with a published RNA-seq profiling dataset of human embryos developed in vitro, thereupon exposed to oxidative stress, and we observed that one of the common downregulated genes between this publication and our data, NEDD1, is involved in centrosome structure and function.ConclusionsTherefore, we assessed the presence of supernumerary centrosomes and showed that the percentage of cells with more than two centrosomes increased acutely with H2O2 treatment in hESCs (HUES3 and 7) and in a control somatic cell line (Hs27), inducing a premature entry into senescence.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0602-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 68%

Global gene expression profiling and senescence biomarker analysis of hESC exposed to H2O2 induced non-cytotoxic oxidative stress

et al. 2017

View full text Add to dashboard Cite

show abstract

“…DDX5 has been shown to participate in RNA replication during HCV infection (66). hnRNP L, XRCC6, CNBP, and RSLID1 have either been shown to enhance other IRESs or be involved in translation (67)(68)(69)(70). NONO, which we found had a role in viral replication, has two RNA recognition motifs and a coiled-coiled protein interaction domain (71,72).…”

Section: Discussionmentioning

confidence: 71%

Thiouracil Cross-Linking Mass Spectrometry: a Cell-Based Method To Identify Host Factors Involved in Viral Amplification

Lenarcic

Landry

Greco

et al. 2013

J Virol

View full text Add to dashboard Cite

bEukaryotic RNA viruses are known to utilize host factors; however, the identity of these factors and their role in the virus life cycle remain largely undefined. Here, we report a method to identify proteins bound to the viral RNA during amplification in cell culture: thiouracil cross-linking mass spectrometry (TUX-MS). TUX-MS relies on incorporation of a zero-distance cross-linker into the viral RNA during infection. Proteins bound to viral RNA are cross-linked prior to cell lysis, purified, and identified using mass spectrometry. Using the TUX-MS method, an unbiased screen for poliovirus (PV) host factors was conducted. All host and viral proteins that are known to interact with the poliovirus RNA were identified. In addition, TUX-MS identified an additional 66 host proteins that have not been previously described in poliovirus amplification. From these candidates, eight were selected and validated. Furthermore, we demonstrate that small interfering RNA (siRNA)-mediated knockdown of two of these uncharacterized host factors results in either a decrease in copy number of positive-stranded RNA or a decrease in PV translation. These data demonstrate that TUX-MS is a robust, unbiased method to identify previously unknown host cell factors that influence virus growth. This method is broadly applicable to a range of RNA viruses, such as flaviviruses, alphaviruses, picornaviruses, bunyaviruses, and coronaviruses.

show abstract

“…CNBP was also found to enhance the cap-independent translation of reporter constructs containing ornithine decarboxylase (ODC) internal ribosome entry site (IRES), suggesting a function as a IRES trans -acting factor (ITAF) (Gerbasi and Link 2007). The yeast homolog of CNBP, Gis2, associates with PABPC1 and the translational machinery and localizes to stress-induced cytoplasmic granules, which further supports a potential function for CNBP in translational regulation (Rojas et al 2012). …”

Section: Introductionmentioning

confidence: 60%

The Human CCHC-type Zinc Finger Nucleic Acid-Binding Protein Binds G-Rich Elements in Target mRNA Coding Sequences and Promotes Translation

et al. 2017

View full text Add to dashboard Cite

Summary The CCHC-type Zinc Finger Nucleic Acid Binding Protein (CNBP/ZNF9) is conserved in eukaryotes and essential for embryonic development in mammals. It has been implicated in transcriptional as well as post-transcriptional gene regulation; however, its nucleic acid ligands and molecular function remain elusive. Here, we use multiple systems-wide approaches to identify CNBP targets and function. We used Photoactivatable Ribonucleoside Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) to identify 8420 CNBP binding sites on 4178 mRNAs. CNBP preferentially bound G-rich elements in the target mRNA coding sequences, most of which were previously found to form G-quadruplex and other stable structures in vitro. Functional analyses, including RNA sequencing, ribosome profiling, and quantitative mass spectrometry, revealed that CNBP binding did not influence target mRNA abundance but rather increased their translational efficiency. Considering that CNBP binding prevented G quadruplex structure formation in vitro, we hypothesize that CNBP is supporting translation by resolving stable structures on mRNAs.

show abstract

Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules

Cited by 30 publications

References 72 publications

Global gene expression profiling and senescence biomarker analysis of hESC exposed to H2O2 induced non-cytotoxic oxidative stress

Global gene expression profiling and senescence biomarker analysis of hESC exposed to H2O2 induced non-cytotoxic oxidative stress

Thiouracil Cross-Linking Mass Spectrometry: a Cell-Based Method To Identify Host Factors Involved in Viral Amplification

The Human CCHC-type Zinc Finger Nucleic Acid-Binding Protein Binds G-Rich Elements in Target mRNA Coding Sequences and Promotes Translation

Contact Info

Product

Resources

About