Yeast Ras Regulates the Complex that Catalyzes the First Step in GPI-Anchor Biosynthesis at the ER

Sobering, Andrew K.; Watanabe, Reika; Romeo, Martin J.; Yan, Benjamin C.; Specht, Charles A.; Orlean, Peter; Riezman, Howard; Levin, David E.

doi:10.1016/j.cell.2004.05.003

Cited by 64 publications

(94 citation statements)

References 41 publications

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“…In agreement with these results, other genes in GPI anchor biosynthesis, including GPI1 (33), and components of the saline response (34) earlier have been linked to the activity of the PKA pathway.…”

Section: Identification Of Membrane Proteins Conferring Stress Resistsupporting

confidence: 75%

Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae

Österberg

Kim

Warringer

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Large-scale protein overexpression phenotype screens provide an important complement to the more common gene knockout screens. Here, we have targeted the so far poorly understood Saccharomyces cerevisiae membrane proteome and report growth phenotypes for a strain collection overexpressing Ϸ600 C-terminally tagged integral membrane proteins grown both under normal and three different stress conditions. Although overexpression of most membrane proteins reduce the growth rate in synthetic defined medium, we identify a large number of proteins that, when overexpressed, confer specific resistance to various stress conditions. Our data suggest that regulation of glycosylphosphatidylinositol anchor biosynthesis and the Na ؉ ͞K ؉ homeostasis system constitute major downstream targets of the yeast PKA͞RAS pathway and point to a possible connection between the early secretory pathway and the cells' response to oxidative stress. We also have quantified the expression levels for >550 membrane proteins, facilitating the choice of well expressing proteins for future functional and structural studies.caffeine ͉ paraquat ͉ salt tolerance ͉ yeast

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Section: Identification Of Membrane Proteins Conferring Stress Resistsupporting

confidence: 75%

Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae

Österberg

Kim

Warringer

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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“…In yeast, interaction of Ras2p with the GPI-GnT complex leads to a mutual negative regulation of GPI biosynthesis and Ras signalling (Sobering et al, 2003(Sobering et al, , 2004. Mutants in GPI biosynthesis often display phenotypes similar to those of hyperactive Ras.…”

Section: Discussionmentioning

confidence: 99%

“…until OD 600 of 2.0. Microsomes were prepared and GPI-GnT activity was assayed for each of them as described by Sobering et al (2004) and references therein.…”

Section: Methodsmentioning

confidence: 99%

The Candida albicans homologue of PIG-P, CaGpi19p: gene dosage and role in growth and filamentation

2010

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Glycosylphosphatidyl inositol (GPI)-anchored proteins in Candida albicans are responsible for a vast range of functions, and deletions in certain GPI-anchored proteins severely reduce adhesion and virulence of this organism. In addition, completely modified GPIs are necessary for virulence. GPI anchor biosynthesis is essential for viability and starts with the transfer of N-acetylglucosamine to phosphatidylinositol. This step is catalysed by a multi-subunit complex, GPI–N-acetylglucosaminyltransferase (GPI–GnT). In this, the first report to our knowledge on a subunit of the Candida GPI–GnT complex, we show that CaGpi19p is the functional equivalent of the Saccharomyces cerevisiae Gpi19p. An N-terminal truncation mutant of CaGpi19p functionally complements a conditionally lethal S. cerevisiae gpi19 mutant. Further, we constructed a conditional null mutant of CaGPI19 by disrupting one allele and placing the remaining copy under the control of the MET3 promoter. Repression leads to growth defects, cell wall biogenesis aberrations, azole sensitivity and hyperfilamention. In addition, there is a noticeable gene dosage effect, with the heterozygote also displaying intermediate degrees of most phenotypes. The mutants also displayed a reduced susceptibility to the antifungal agent amphotericin B. Collectively, the results suggest that CaGPI19 is required for normal morphology and cell wall architecture.

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“…GlcNAc transfer occurs at the cytoplasmic face of the ER membrane (Vidugiriene and Menon 1993;Watanabe et al 1996;Tiede et al 2000). Essential Gpi2, Gpi15, and Gpi19 (Leidich et al 1995;Yan et al 2001;Newman et al 2005), and nonessential Gpi1 and Eri1 (Leidich and Orlean 1996;Sobering et al 2004), are also required for GlcNAc-PI synthesis. ERI1 and GPI1 null mutants are temperature-sensitive.…”

Section: Gpi Anchoringmentioning

confidence: 99%

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

2012

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The wall gives a Saccharomyces cerevisiae cell its osmotic integrity; defines cell shape during budding growth, mating, sporulation, and pseudohypha formation; and presents adhesive glycoproteins to other yeast cells. The wall consists of b1,3-and b1,6-glucans, a small amount of chitin, and many different proteins that may bear N-and O-linked glycans and a glycolipid anchor. These components become cross-linked in various ways to form higher-order complexes. Wall composition and degree of cross-linking vary during growth and development and change in response to cell wall stress. This article reviews wall biogenesis in vegetative cells, covering the structure of wall components and how they are cross-linked; the biosynthesis of N-and O-linked glycans, glycosylphosphatidylinositol membrane anchors, b1,3-and b1,6-linked glucans, and chitin; the reactions that cross-link wall components; and the possible functions of enzymatic and nonenzymatic cell wall proteins. TABLE OF CONTENTS Abstract 775Introduction 777

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Yeast Ras Regulates the Complex that Catalyzes the First Step in GPI-Anchor Biosynthesis at the ER

Cited by 64 publications

References 41 publications

Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae

Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae

The Candida albicans homologue of PIG-P, CaGpi19p: gene dosage and role in growth and filamentation

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

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