Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp.

Abstract

In the present study, it was sought to compare yeast microbiota of wild and captive Macrobrachium amazonicum and evaluate the antifungal susceptibility and production of virulence factors by the recovered isolates of Candida spp. Additionally, cultivation water was monitored for the presence of fungi. Overall, 26 yeast isolates belonging to three genera and seven species were obtained, out of which 24 were Candida spp., with Candida famata as the most prevalent species for both wild and captive prawns. From cultivation water, 28 isolates of filamentous fungi were obtained, with Penicillium spp., Cladosporium spp. and Aspergillus spp. as the most frequent genera. Eight out of 24 Candida spp. isolates were resistant to azole derivatives, out of which four were recovered from wild-harvested prawns. As for production of virulence factors, three (12.5%) and eight (33.3%) isolates presented phospholipase and protease activity, respectively. This is the first comparative study between wild and captive prawns and the first report on yeast microbiota of M. amazonicum. The most relevant finding was the high percentage of resistant Candida spp., including from wild individuals, which suggests the occurrence of an environmental imbalance in the area where these prawns were captured.

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

“…The genera Cryptococcus and Candida are known to contain true marine yeasts (Kutty and Philip 2008) and the species C. sake is present in various aquatic environments including lakes, lagoons, and Antarctic seawater (Boguslawska-Was and Dabrowski 2001;Brandão et al 2011;Buzzini et al 2012). Furthermore, members of this species are pathogens of freshwater prawn, fish, and marine invertebrates (Hatai and Egusa 1975;de Araujo et al 1995;Brilhante et al 2011). …”

Macrobrachium amazonicum: an alternative for microbiological monitoring of aquatic environments in Brazil

“…degradation (MEDEIROS et al, 2008;BRILHANTE et al, 2011BRILHANTE et al, , 2012CASTELO-BRANCO et al, 2013), particularly in samples obtained from aquatic sources (BUTINAR et al, 2005;MEDEIROS et al, 2008). In these studies, the isolation of this genus was greater than that of other microorganisms, including bacteria, demonstrating the potential use of this yeast for environmental monitoring.…”

“…It is delimited by the UTM coordinates 0567000E, 9561273N and 0575000E, 9569000N. It is a rich freshwater body with mangrove areas that have been degraded by uncontrolled occupation of the surrounding area and pollution with residues from industrial, commercial and farming activities (BRILHANTE et al, 2011).…”

“…Afterwards, the digestive tracts of 10 individuals were removed, placed in sterile slants containing sterile saline and treated as one single sample (BRILHANTE et al, 2011). Overall, 12 collections were performed.…”

“…Hence, it is important to seek alternatives to facilitate monitoring of aquatic ecosystems. In this context, the use of aquatic crustaceans has been reported as a reliable alternative for that purpose, especially because of their feeding habits (fi lter feeding) and benthic behavior, as described by VIRGA et al (2007) andBRILHANTE et al (2011).…”