Yellow Chaste Weed and Its Components, Apigenin and Galangin, Affect Proliferation and Oxidative Stress in Blue Light-Irradiated HaCaT Cells

Abstract

While harmful effects of blue light on skin cells have been recently reported, there are few studies regarding natural products that alleviate its negative effects. Therefore, we investigated ameliorating effects of yellow chaste weed (YCW) (Helichrysum arenarium) extract and its components, apigenin and galangin, on blue light-irradiated HaCaT cells. In this study, we found that YCW extract improved the reduced proliferation of HaCaT cells induced by blue light-irradiation and reduced blue light-induced production of reactive oxygen species (ROS) levels. We also found that apigenin and galangin, the main components of YCW extract, showed the same activities as YCW extract. In experiments examining molecular mechanisms of YCW extract and its components such as apigenin and galangin, they all reduced expression of transient receptor potential vanilloid member 1 (TRPV1), its phosphorylation, and calcium ion (Ca2+) influx induced by blue light irradiation. In addition, apigenin and galangin regulated phosphorylation of mitogen-activated protein kinases (MAPKs). They also reduced phosphorylation of mammalian sterile 20-like kinase-1/2 (MST-1/2), inducing phosphorylation of Akt (protein kinase B), one downstream molecule of MST-1/2. Moreover, apigenin and galangin promoted translocation of Forkhead box O3 (FoxO3a) from the nucleus to the cytosol by phosphorylating FoxO3a. Besides, apigenin and galangin interrupted blue light influences on expression of nuclear and secretory clusterin. Namely, they attenuated both upregulation of nuclear clusterin and downregulation of secretory clusterin induced by blue light irradiation. We also found that they downregulated apoptotic protein Bcl-2 associated X protein (Bax) and conversely upregulated anti-apoptotic protein B-cell lymphoma 2 (Bcl-2). Collectively, these findings indicate that YCW extract and its components, apigenin and galangin, antagonize the blue light-induced damage to the keratinocytes by regulating TRPV1/clusterin/FoxO3a and MAPK signaling.

Naringin acts as a TRPV1 antagonist to attenuate UVB‐induced senescence and damage in HaCaT cells

“…Hence, Nar could effectively inhibit UVB‐induced apoptosis of HaCaT cells and promote cell proliferation. Cap, an irritating natural compound, can activate TRPV1, increase intracellular calcium concentration, and activate various apoptosis signaling pathways, thereby inducing apoptosis and cell cycle arrest of HaCaT cells (Park et al., 2022). We showed that treatment with the TRPV1 activator Cap inhibited HaCaT cell proliferation and promoted apoptosis.…”

The effect on the organism of rats of adding Helichrysum arenarium inflorescences to a hypercaloric diet, high in sugar and fat

“…The presence of antitumour activity of H. arenarium against transplanted sarcoma 45 while using immortelle extract was also indicated (Grinev et al, 2016). The extract of H. arenarium and its active components apigenin and galangin showed a significant protective effect on skin cells exposed to blue light (Park et al, 2022). Pospelov et al (2019) studied the biological activity of the native immortelle extract and its fraction with and without lectins in inhibiting the germination of teliospores Ustilago nuda.…”

Functional Properties of Natural Products and Human Health

“…Park et al [4] demonstrated the ability of phenolic compounds to prevent the negative effects on the skin of blue light, also known as high-energy visible (HEV) light. Particularly, they studied the protective effects of yellow chaste weed (YCW) (Helichrysum arenarium) extract and its main components, apigenin and galangin, on HEV light-irradiated HaCaT keratinocytes from human skin.…”