YieJ (CbrC) Mediates CreBC-Dependent Colicin E2 Tolerance in
            <i>Escherichia coli</i>

Cariss, Sjl; Constantinidou, Chrystala; Patel, Mala D.; Takebayashi, Yuiko; Hobman, Jon L.; Penn, Charles W.; Avison, Matthew B.

doi:10.1128/jb.01352-09

Cited by 19 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, pcbrA-lacZ transcription was upregulated in LB and M9 media by creB, as revealed by the creB mutant with low expression (Table 4), and was correlated with the degree of Cma sensitivity (Table 3). CreBC-dependent upregulation of cbrA expression has been observed in reverse transcription-PCR (RT-PCR) and DNA microarray analyses, and our data supplement these earlier observations (2,7).…”

Section: Discussionsupporting

confidence: 80%

“…Recently, another CreBC-regulated protein, CbrC (YieJ), that increases resistance to colicin E2 has been identified (7). A 95-fold overexpression of cbrC in a mutant carrying a point mutation in the CreC histidine kinase domain leads to survival at a colicin E2 concentration 16-fold higher than the concentration survived by the CreC parent strain.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

CbrA Is a Flavin Adenine Dinucleotide Protein That Modifies the Escherichia coli Outer Membrane and Confers Specific Resistance to Colicin M

et al. 2012

View full text Add to dashboard Cite

ABSTRACTColicin M (Cma) is a protein toxin produced byEscherichia colithat kills sensitiveE. colicells by inhibiting murein biosynthesis in the periplasm. Recombinant plasmids carryingcbrA(formerlyyidS) strongly increased resistance of cells to Cma, whereas deletion ofcbrAincreased Cma sensitivity. Transcription ofcbrAis positively controlled by the two-component CreBC system. A ΔcreBmutant was highly Cma sensitive because little CbrA was synthesized. Treatment of CbrA-overproducing cells by osmotic shock failed to render cells Cma sensitive because the cells were resistant to osmotic shock. In a natural environment with a growth-limiting nutrient supply, cells producing CbrA defend themselves against colicin M synthesized by competing cells. Isolated CbrA is a protein with noncovalently bound flavin adenine dinucleotide. Sequence comparison and structure prediction assign the closest relative of CbrA with a known crystal structure as digeranylgeranyl-glycerophospholipid reductase ofThermoplasma acidophilum. CbrA is found inEscherichia coli,Citrobacter, andSalmonella bongoribut not in other enterobacteria. The next homologs with the highest identity (over 50%) are found in the anaerobicClostridium botulinumgroup 1 and a few otherFirmicutes.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

CbrA Is a Flavin Adenine Dinucleotide Protein That Modifies the Escherichia coli Outer Membrane and Confers Specific Resistance to Colicin M

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In addition to the perspective on metabolism, a recent report indicated that the activation of the CreBC system is related to the tolerance of E2 colicin (47). Colicins are protein antibiotics produced by specific strains of E. coli and are toxic for some bacterial strains.…”

Section: Fig 5 the Kinase Assaysmentioning

confidence: 99%

Lactoferricin B Inhibits the Phosphorylation of the Two-Component System Response Regulators BasR and CreB

Sung

Chen

2012

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Natural antimicrobial peptides provide fundamental protection for multicellular organisms from microbes, such as Lactoferricin B (Lfcin B). Many studies have shown that Lfcin B penetrates the cell membrane and has intracellular activities. To elucidate the intracellular behavior of Lfcin B, we first used Escherichia coli K12 proteome chips to identify the intracellular targets of Lfcin B. The results showed that Lfcin B binds to two response regulators, BasR and CreB, of the two-component system. For further analysis, we conducted several in vitro and in vivo experiments and utilized bioinformatics methods. The electrophoretic mobility shift assays and kinase assays indicate that Lfcin B inhibits the phosphorylation of the response regulators (BasR and CreB) and their cognate sensor kinases (BasS and CreC). Antibacterial assays showed that Lfcin B reduced E. coli's tolerance to environmental stimuli, such as excessive ferric ions and minimal medium conditions. This is the first study to show that an antimicrobial peptide inhibits the growth of bacteria by influencing

show abstract

“…A direct-repeat consensus DNA sequence, termed the cre tag (5=-TCACnnnnnnTTCAC-3=, where n represents any nucleotide), was defined based on analysis of the region upstream from the genes known to form the cre regulon and was observed to be required for the control of gene expression in vivo (7). Genome-wide expression profiling with DNA microarrays has revealed that CreBC also affects the expression of the following genes: cbrC, responsible for colicine E2 resistance; mokB, encoding an overlapping regulatory peptide that enables hokB expression; and the uncharacterized genes mppA, ynaI, yafU, and yafE (12).…”

mentioning

confidence: 99%

The CreC Regulator of Escherichia coli, a New Target for Metabolic Manipulations

Godoy

Nikel

Gomez

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism. The impacts of different creC mutations (a ⌬creC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology were analyzed in glucose cultures under three oxygen availability conditions. Differences in the amounts of extracellular metabolites produced were observed in the null mutant compared to the wild-type strain and the mutant carrying creC510 and shown to be affected by oxygen availability. The ⌬creC strain secreted more formate, succinate, and acetate but less lactate under low aeration. These metabolic changes were associated with differences in AckA and LdhA activities, both of which were affected by CreC. Measurement of the NAD(P)H/NAD(P) ؉ ratios showed that the creC510 strain had a more reduced intracellular redox state, while the opposite was observed for the ⌬creC mutant, particularly under intermediate oxygen availability conditions, indicating that CreC affects redox balance. The null mutant formed more succinate than the wild-type strain under both low aeration and no aeration. Overexpression of the genes encoding phosphoenolpyruvate carboxylase from E. coli and a NADH-forming formate dehydrogenase from Candida boidinii in the ⌬creC mutant further increased the yield of succinate on glucose. Interestingly, the elimination of ackA and adhE did not significantly improve the production of succinate. The diverse metabolic effects of this regulator on the central biochemical network of E. coli make it a good candidate for metabolic-engineering manipulations to enhance the formation of bioproducts, such as succinate.T he survival of an organism depends, at least in part, on its ability to sense and respond to changes in the environment. In bacteria, global regulators control the transcription of genes in response to specific external stimuli and metabolic signals, finely tuning different aspects of their physiology to overcome environmental challenges. In Escherichia coli, seven global regulators (ArcA, Crp, Fis, Fnr, Ihf, Lrp, and NarL) directly modulate the expression of about one-half of all genes (1). This facultative aerobe is able to adapt its metabolism to different oxygen availability conditions through the concerted actions of a network of regulators, including the global regulators ArcAB and Fnr (2-4). These regulators affect many metabolic pathways, allowing the cells to reach an adequate redox balance under any given conditions. There is a very close association between carbon and electron flows, and even small differences in oxygen availability have been observed to elicit profound effects on the distribution of carbon fluxes (5).CreBC (for carbon source responsive) is a global sensing and regulation system affecting genes involved in a variety of functions, including enzymes of intermediary catabolism (6). Previous studies have shown that the creABCD operon is activated (i) during growth in minimal mediu...

show abstract

YieJ (CbrC) Mediates CreBC-Dependent Colicin E2 Tolerance in Escherichia coli

Cited by 19 publications

References 30 publications

CbrA Is a Flavin Adenine Dinucleotide Protein That Modifies the Escherichia coli Outer Membrane and Confers Specific Resistance to Colicin M

CbrA Is a Flavin Adenine Dinucleotide Protein That Modifies the Escherichia coli Outer Membrane and Confers Specific Resistance to Colicin M

Lactoferricin B Inhibits the Phosphorylation of the Two-Component System Response Regulators BasR and CreB

The CreC Regulator of Escherichia coli, a New Target for Metabolic Manipulations

Contact Info

Product

Resources

About