Direct wafer bonding was performed under medium vacuum conditions. The effects of the sequence of wafer contact and vacuum application, annealing temperature, and annealing time on the bonding quality and bonding speed were investigated. From the comparison of the bonding efficiency, contacting two wafers in air rather than in vacuum produces much less bubbles. For vacuum bonding, good bonding, such as high bonding strength and high bonding efficiency, can be achieved at temperatures as low as 300 • C. From the comparison between air wafer bonding and medium vacuum wafer bonding, it is obvious that medium vacuum annealing can improve the bonding strength and accelerate the bonding process. Based on the results observed, a qualitative mechanism of medium vacuum wafer bonding at low temperatures was proposed.