ZBP-89 reduces the cell death threshold in hepatocellular carcinoma cells by increasing caspase-6 and S phase cell cycle arrest

Chen, George G.; Chan, Ursula P. F.; Bai, Long; Fung, King Y.; Tessier, Art; To, Ann K Y; Merchant, Juanita L.; Lai, Paul B.S.

doi:10.1016/j.canlet.2009.03.024

Cited by 19 publications

(17 citation statements)

References 36 publications

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“…10 Moreover, overexpression of ZBP-89 is reported to significantly promote cell death in five HCC cell lines. 23 In line with the previous reports, our data showed that overexpression of ZBP-89 in HCC facilitated cell death induced by TSA via enhanced activation of Bid and PARP. However, overexpression of ZBP-89-Δ6-180 protein did not potentiate TSA-induced apoptosis, suggesting that the N terminus of ZBP-89 is essential for apoptosis induction.…”

Section: Discussionsupporting

confidence: 82%

“…We have previously reported that ZBP-89 overexpression resulted in apoptosis. 10,23 Using in situ cell death detection and TUNEL assays, we found that ectopic expression of ZBP-89 increased apoptosis ( Fig. 6A and B).…”

Section: Hdaci Induces Cell Cycle Arrest and Apoptosis In Hcc Cellsmentioning

confidence: 99%

“…In our early study, we demonstrated that ZBP-89 enhanced the lethal effectiveness of 5-fluorouracil or staurosporine in HCC cells. 23 Thus, we intended to test whether overexpression of ZBP-89 influenced p53 G245D -mediated resistance to TSA-induced apoptosis. Results showed that ZBP-89 overexpression in Hep3B-G245D cells enhanced sensitivity to TSA treatment ( Fig.…”

Section: Hdaci Induces Cell Cycle Arrest and Apoptosis In Hcc Cellsmentioning

confidence: 99%

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Interaction between ZBP-89 and p53 mutants and its contribution to effects of HDACi on hepatocellular carcinoma

et al. 2012

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Section: Discussionsupporting

confidence: 82%

Section: Hdaci Induces Cell Cycle Arrest and Apoptosis In Hcc Cellsmentioning

confidence: 99%

Section: Hdaci Induces Cell Cycle Arrest and Apoptosis In Hcc Cellsmentioning

confidence: 99%

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Interaction between ZBP-89 and p53 mutants and its contribution to effects of HDACi on hepatocellular carcinoma

et al. 2012

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“…This cell line has a mutation in the p53 gene that confers overexpression of p53 protein [16]. Overexpression of the tumor suppressor protein might be responsible for the greater cell damage induced by the chemotherapy and, consequently, the increased cell death [20,21]. …”

Section: Discussionmentioning

confidence: 99%

Hepatocellular Carcinoma and Chemotherapy: The Role of p53

Brito¹,

Abrantes²,

Pinto-Costa³

et al. 2012

Chemotherapy

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Background: Hepatocellular carcinoma (HCC) is the most common primary neoplasm of the liver. A major proportion of HCCs also present mutation of the gene that encodes p53, which confers chemoresistance. The main goal of this work is to investigate the effect of cisplatin, doxorubicin and 5-fluoruracil (5-FU) in three human HCC cell lines which differ in p53 expression. Methods: HepG2 (expressing normal p53), HuH7 (expressing mutated p53) and Hep3B2.1-7 (not expressing p53) cell lines were cultivated in the presence of cisplatin, doxorubicin and 5-FU. Cell proliferation was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The type of cell death and Bax and Bcl2 activation were assessed by flow cytometry. Results: It was found that for all of the cell lines studied, the agent that gave the most satisfactory results was doxorubicin. 5-FU demonstrated no activity in these cell lines. Conclusions: For all the cell lines studied, doxorubicin was the most satisfactory agent. In HepG2 and HuH7 cell lines, it can activate Bax with statistical significance.

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“…[3][4][5][6][7] ZNF148 is universally expressed at low levels in most tissues, 8 including those in the gastrointestinal tract. 9 ZNF148 is a multifunctional transcription factor associated with signaling pathways upstream of cellular proliferation, embryogenesis, differentiation, growth arrest, and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Expression of ZNF148 in different developing stages of colorectal cancer and its prognostic value

Xian

Liu

Chang

et al. 2013

Cancer

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BACKGROUND: It has been speculated that zinc finger protein 148 (ZNF148) is a tumor suppressor. However, to the authors' knowledge, little is known about the clinical significance of ZNF148 expression in patients with colorectal cancer (CRC). The objective of the current study was to clarify the association between ZNF148 expression and the postoperative prognosis of patients with CRC. METHODS: Tissue microarrays containing 56 normal mucosa, 51 adenoma, 742 CRC (TNM stage I-IV), 16 familial adenomatous polyposis, and 21 metastatic CRC specimens were examined immunohistochemically for ZNF148 expression. RESULTS: Expression of ZNF148 was found to increase consecutively from normal mucosa to stage I CRC, and then decreased consecutively from stage I to stage IV CRC. Lower expression of ZNF148 in tumors was found to be significantly associated with lymph node metastases, advanced TNM disease stage, poor differentiation, higher rate of disease recurrence, worse overall survival (OS), and shorter disease-free survival. High expression of ZNF148 was also associated with improved OS (P 5 .025) and disease-free survival (P 5.042) in patients with stages II to III CRC. On multivariate Cox analysis, lower ZNF148 expression in tumors, advanced TNM stage, colon cancer, and elevated serum carbohydrate antigen 19-9 (CA19-9) were found to be significant factors for a worse OS. In 16 patients with familial adenomatous polyposis, ZNF148 expression was upregulated at steps toward carcinogenesis. In 21 patients with metastatic CRC, although ZNF148 expression was higher in primary tumors compared with adjacent mucosa, its expression in metastatic tumors was significantly lower than that in primary tumors. CONCLUSIONS: Although ZNF148 expression is related to colorectal carcinogenesis, high ZNF148 expression in patients with CRC

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ZBP-89 reduces the cell death threshold in hepatocellular carcinoma cells by increasing caspase-6 and S phase cell cycle arrest

Cited by 19 publications

References 36 publications

Interaction between ZBP-89 and p53 mutants and its contribution to effects of HDACi on hepatocellular carcinoma

Interaction between ZBP-89 and p53 mutants and its contribution to effects of HDACi on hepatocellular carcinoma

Hepatocellular Carcinoma and Chemotherapy: The Role of p53

Expression of ZNF148 in different developing stages of colorectal cancer and its prognostic value

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